Investigation of Fe3O4 nanoparticles synthesized via co-precipitation coated with chitosan, PEG, and folate for targeted paclitaxel delivery in fibrosarcoma treatment

Magnetite (Fe3O4) nanoparticles (NPs) were synthesized using a straightforward co-precipitation method from Fe2+/Fe3+ salt solutions as starting materials. This method was optimized to control particle size and morphology, resulting in highly magnetic NPs with crystallite sizes ranging from 20 to 70...

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Main Authors: Adawiya Haider, Sharafaldin Al-Musawi, Rusul Al-Obaidy, Azhar Haleemd, Mohammed Al-Hindawi, Bakr Taha
Format: Article
Language:English
Published: Unviversity of Technology- Iraq 2025-04-01
Series:Engineering and Technology Journal
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Online Access:https://etj.uotechnology.edu.iq/article_186487_195a277f4aa7f240e3e3303600255e80.pdf
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author Adawiya Haider
Sharafaldin Al-Musawi
Rusul Al-Obaidy
Azhar Haleemd
Mohammed Al-Hindawi
Bakr Taha
author_facet Adawiya Haider
Sharafaldin Al-Musawi
Rusul Al-Obaidy
Azhar Haleemd
Mohammed Al-Hindawi
Bakr Taha
author_sort Adawiya Haider
collection DOAJ
description Magnetite (Fe3O4) nanoparticles (NPs) were synthesized using a straightforward co-precipitation method from Fe2+/Fe3+ salt solutions as starting materials. This method was optimized to control particle size and morphology, resulting in highly magnetic NPs with crystallite sizes ranging from 20 to 70 nm. The synthesis process involved magnetic stirring at 700 rpm at room temperature, yielding approximately 5 mg of Fe3O4 powder. Structural characterization via X-ray diffraction (XRD) confirmed the stoichiometric composition and particle dimensions. To enhance functionality, the Fe3O4 NPs were coated with chitosan (Cs), polyethylene glycol (PEG), and folic acid (FA), and loaded with the anticancer drug paclitaxel (PTX), forming nanoencapsulated Fe3O4@Cs-PEG-PTX-FA particles with sizes ranging from 100 to 130 nm. Magnetic properties were assessed using a vibrating sample magnetometer (VSM), revealing saturation magnetization (Ms) values of 59.6 emu/mg for Fe3O4 and 19.9 emu/mg for Fe3O4@Cs-PEG-PTX-FA. Encapsulation efficiency of 82.4% and drug-loading content of 14.4% were achieved. These results demonstrate the potential of Fe3O4 NPs as multifunctional agents in the biomedical field, particularly for targeted drug delivery systems, where magnetic properties can facilitate precise localization and release of therapeutic agents. In addition to size uniformity, biocompatibility, and responsiveness to external magnetic fields make, these properties make them promising candidates for cancer treatment, particularly in targeting fibrosarcoma cells.
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spelling doaj-art-e4a8f62383c747d8939ead5edf0448bf2025-08-20T01:47:32ZengUnviversity of Technology- IraqEngineering and Technology Journal1681-69002412-07582025-04-0143424425210.30684/etj.2025.155892.1867186487Investigation of Fe3O4 nanoparticles synthesized via co-precipitation coated with chitosan, PEG, and folate for targeted paclitaxel delivery in fibrosarcoma treatmentAdawiya Haider0Sharafaldin Al-Musawi1Rusul Al-Obaidy2Azhar Haleemd3Mohammed Al-Hindawi4Bakr Taha5Applied Sciences Dept., University of Technology- Iraq, Alsina’a street, 10066 Baghdad, Iraq.College of Food Sciences, Al-Qasim Green University, Babylon, Iraq.Minsitry of Industry and Minerals Industrial Development and Regulatory Directorate, Baghdad-Iraq.Environmental Research Center, University of Technology, Alsina’a street, 10066 Baghdad-IraqApplied Sciences Dept., University of Technology- Iraq, Alsina’a street, 10066 Baghdad, Iraq.Photonics Technology Laboratory, Dept., of Electrical, Electronic and Systems Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia UKM, 43600 Bangi, Malaysia.Magnetite (Fe3O4) nanoparticles (NPs) were synthesized using a straightforward co-precipitation method from Fe2+/Fe3+ salt solutions as starting materials. This method was optimized to control particle size and morphology, resulting in highly magnetic NPs with crystallite sizes ranging from 20 to 70 nm. The synthesis process involved magnetic stirring at 700 rpm at room temperature, yielding approximately 5 mg of Fe3O4 powder. Structural characterization via X-ray diffraction (XRD) confirmed the stoichiometric composition and particle dimensions. To enhance functionality, the Fe3O4 NPs were coated with chitosan (Cs), polyethylene glycol (PEG), and folic acid (FA), and loaded with the anticancer drug paclitaxel (PTX), forming nanoencapsulated Fe3O4@Cs-PEG-PTX-FA particles with sizes ranging from 100 to 130 nm. Magnetic properties were assessed using a vibrating sample magnetometer (VSM), revealing saturation magnetization (Ms) values of 59.6 emu/mg for Fe3O4 and 19.9 emu/mg for Fe3O4@Cs-PEG-PTX-FA. Encapsulation efficiency of 82.4% and drug-loading content of 14.4% were achieved. These results demonstrate the potential of Fe3O4 NPs as multifunctional agents in the biomedical field, particularly for targeted drug delivery systems, where magnetic properties can facilitate precise localization and release of therapeutic agents. In addition to size uniformity, biocompatibility, and responsiveness to external magnetic fields make, these properties make them promising candidates for cancer treatment, particularly in targeting fibrosarcoma cells.https://etj.uotechnology.edu.iq/article_186487_195a277f4aa7f240e3e3303600255e80.pdffe3o4 nps fibrosarcoma chitosan ptx coprecipitation
spellingShingle Adawiya Haider
Sharafaldin Al-Musawi
Rusul Al-Obaidy
Azhar Haleemd
Mohammed Al-Hindawi
Bakr Taha
Investigation of Fe3O4 nanoparticles synthesized via co-precipitation coated with chitosan, PEG, and folate for targeted paclitaxel delivery in fibrosarcoma treatment
Engineering and Technology Journal
fe3o4 nps fibrosarcoma chitosan ptx co
precipitation
title Investigation of Fe3O4 nanoparticles synthesized via co-precipitation coated with chitosan, PEG, and folate for targeted paclitaxel delivery in fibrosarcoma treatment
title_full Investigation of Fe3O4 nanoparticles synthesized via co-precipitation coated with chitosan, PEG, and folate for targeted paclitaxel delivery in fibrosarcoma treatment
title_fullStr Investigation of Fe3O4 nanoparticles synthesized via co-precipitation coated with chitosan, PEG, and folate for targeted paclitaxel delivery in fibrosarcoma treatment
title_full_unstemmed Investigation of Fe3O4 nanoparticles synthesized via co-precipitation coated with chitosan, PEG, and folate for targeted paclitaxel delivery in fibrosarcoma treatment
title_short Investigation of Fe3O4 nanoparticles synthesized via co-precipitation coated with chitosan, PEG, and folate for targeted paclitaxel delivery in fibrosarcoma treatment
title_sort investigation of fe3o4 nanoparticles synthesized via co precipitation coated with chitosan peg and folate for targeted paclitaxel delivery in fibrosarcoma treatment
topic fe3o4 nps fibrosarcoma chitosan ptx co
precipitation
url https://etj.uotechnology.edu.iq/article_186487_195a277f4aa7f240e3e3303600255e80.pdf
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