Proteomic analysis of premature umbilical cord blood and its relationship with bronchopulmonary dysplasia
Abstract Background Bronchopulmonary dysplasia (BPD) frequently occurs in preterm infants, causing significantly impaired lung function and increased mortality rates. Studies on plasma protein levels can facilitate early detection of BPD, enabling prompt intervention and a decrease in mortality. Met...
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BMC
2025-03-01
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| Series: | Italian Journal of Pediatrics |
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| Online Access: | https://doi.org/10.1186/s13052-025-01926-8 |
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| author | Jia Chen Yuanye He Ying Liu Zhiwei Guo Longli Yan Xiaotao Jiang Weiwei Gao |
| author_facet | Jia Chen Yuanye He Ying Liu Zhiwei Guo Longli Yan Xiaotao Jiang Weiwei Gao |
| author_sort | Jia Chen |
| collection | DOAJ |
| description | Abstract Background Bronchopulmonary dysplasia (BPD) frequently occurs in preterm infants, causing significantly impaired lung function and increased mortality rates. Studies on plasma protein levels can facilitate early detection of BPD, enabling prompt intervention and a decrease in mortality. Methods We conducted a prospective observational study involving proteomic sequencing of plasma samples from 19 preterm infants. Our analysis included principal component analysis, volcano plots, heatmap analysis, enrichment analysis, and receiver operating characteristic (ROC) analysis. Results Infants with BPD were characterized by increased levels of lipopolysaccharide (LPS)-binding protein (LBP), X-ray repair cross-complementing protein 6 (XRCC6), GLI pathogenesis-related 1 (GLIPR1), Golgi membrane Protein 1(GOLM1), immunoglobulin kappa variable (IGKV1-5), and immunoglobulin kappa variable 1–33 (IGKV1-33) in cord blood. Additionally, gene pathway analysis revealed a significant correlation between the pathways associated with these genes and BPD, particularly pathways involved in the immune system, innate immune system, neutrophil degranulation, prion diseases, regulation of the actin cytoskeleton, and the MAPK signaling. The proteins amine oxidase copper containing 3 (AOC3) and H4 clustered histone 6 (H4C6) were diagnostically significant. Additionally, H4C6 was negatively correlated with intraventricular haemorrhage and patent ductus arteriosus, and positively correlated with antenatal steroid administration. AOC3 was also positively correlated with antenatal steroid use. Conclusions Our findings suggest that the development of BPD is associated with changes in the plasma proteome of preterm infants. Specifically, the levels of AOC3 and H4C6 in the bloodstream could serve as biomarkers for the early detection of BPD in preterm infants. Furthermore, we found that GOLM1, lipopolysaccharide (LPS)-binding protein, XRCC6, and the contribution of neutrophil degranulation may play a crucial role in the development of therapies for BPD. |
| format | Article |
| id | doaj-art-e49d44ff9caf40a4a3f64300a24c421a |
| institution | DOAJ |
| issn | 1824-7288 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Italian Journal of Pediatrics |
| spelling | doaj-art-e49d44ff9caf40a4a3f64300a24c421a2025-08-20T02:41:34ZengBMCItalian Journal of Pediatrics1824-72882025-03-0151111210.1186/s13052-025-01926-8Proteomic analysis of premature umbilical cord blood and its relationship with bronchopulmonary dysplasiaJia Chen0Yuanye He1Ying Liu2Zhiwei Guo3Longli Yan4Xiaotao Jiang5Weiwei Gao6National Key Clinical Specialty Construction Project/Neonatology Department, Guangdong Women and Children HospitalDepartment of Laboratory Medicine, Guangdong Second Provincial General HospitalNational Key Clinical Specialty Construction Project/Neonatology Department, Guangdong Women and Children HospitalCenter for Medical Research On Innovation and Translation, Institute of Clinical Medicine, School of Medicine, Guangzhou First People’s Hospital, South China University of TechnologyNational Key Clinical Specialty Construction Project/Neonatology Department, Guangdong Women and Children HospitalFirst College of Clinic Medicine, Guangzhou University of Chinese MedicineNational Key Clinical Specialty Construction Project/Neonatology Department, Guangdong Women and Children HospitalAbstract Background Bronchopulmonary dysplasia (BPD) frequently occurs in preterm infants, causing significantly impaired lung function and increased mortality rates. Studies on plasma protein levels can facilitate early detection of BPD, enabling prompt intervention and a decrease in mortality. Methods We conducted a prospective observational study involving proteomic sequencing of plasma samples from 19 preterm infants. Our analysis included principal component analysis, volcano plots, heatmap analysis, enrichment analysis, and receiver operating characteristic (ROC) analysis. Results Infants with BPD were characterized by increased levels of lipopolysaccharide (LPS)-binding protein (LBP), X-ray repair cross-complementing protein 6 (XRCC6), GLI pathogenesis-related 1 (GLIPR1), Golgi membrane Protein 1(GOLM1), immunoglobulin kappa variable (IGKV1-5), and immunoglobulin kappa variable 1–33 (IGKV1-33) in cord blood. Additionally, gene pathway analysis revealed a significant correlation between the pathways associated with these genes and BPD, particularly pathways involved in the immune system, innate immune system, neutrophil degranulation, prion diseases, regulation of the actin cytoskeleton, and the MAPK signaling. The proteins amine oxidase copper containing 3 (AOC3) and H4 clustered histone 6 (H4C6) were diagnostically significant. Additionally, H4C6 was negatively correlated with intraventricular haemorrhage and patent ductus arteriosus, and positively correlated with antenatal steroid administration. AOC3 was also positively correlated with antenatal steroid use. Conclusions Our findings suggest that the development of BPD is associated with changes in the plasma proteome of preterm infants. Specifically, the levels of AOC3 and H4C6 in the bloodstream could serve as biomarkers for the early detection of BPD in preterm infants. Furthermore, we found that GOLM1, lipopolysaccharide (LPS)-binding protein, XRCC6, and the contribution of neutrophil degranulation may play a crucial role in the development of therapies for BPD.https://doi.org/10.1186/s13052-025-01926-8Bronchopulmonary dysplasiaPlasma proteomicsPreterm infantsBiomarkers |
| spellingShingle | Jia Chen Yuanye He Ying Liu Zhiwei Guo Longli Yan Xiaotao Jiang Weiwei Gao Proteomic analysis of premature umbilical cord blood and its relationship with bronchopulmonary dysplasia Italian Journal of Pediatrics Bronchopulmonary dysplasia Plasma proteomics Preterm infants Biomarkers |
| title | Proteomic analysis of premature umbilical cord blood and its relationship with bronchopulmonary dysplasia |
| title_full | Proteomic analysis of premature umbilical cord blood and its relationship with bronchopulmonary dysplasia |
| title_fullStr | Proteomic analysis of premature umbilical cord blood and its relationship with bronchopulmonary dysplasia |
| title_full_unstemmed | Proteomic analysis of premature umbilical cord blood and its relationship with bronchopulmonary dysplasia |
| title_short | Proteomic analysis of premature umbilical cord blood and its relationship with bronchopulmonary dysplasia |
| title_sort | proteomic analysis of premature umbilical cord blood and its relationship with bronchopulmonary dysplasia |
| topic | Bronchopulmonary dysplasia Plasma proteomics Preterm infants Biomarkers |
| url | https://doi.org/10.1186/s13052-025-01926-8 |
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