Evaluation of a novel liquid-solid biphasic differential medium for screening of group B streptococcus in perinatal women
ABSTRACT This study explored the capabilities of the commonly used blood agar plate in Chinese clinical microbiology laboratories and a novel liquid-solid biphasic differential medium (L-S StrepB) for detecting group B streptococcus (GBS) in peripartum women in China. A total of 11.2% (65/582) of pr...
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| Language: | English |
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American Society for Microbiology
2025-06-01
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| Series: | Microbiology Spectrum |
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| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.00140-25 |
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| _version_ | 1849735048232697856 |
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| author | Shuo Chen Fangqu Li Xiangyang Li Hao Chen |
| author_facet | Shuo Chen Fangqu Li Xiangyang Li Hao Chen |
| author_sort | Shuo Chen |
| collection | DOAJ |
| description | ABSTRACT This study explored the capabilities of the commonly used blood agar plate in Chinese clinical microbiology laboratories and a novel liquid-solid biphasic differential medium (L-S StrepB) for detecting group B streptococcus (GBS) in peripartum women in China. A total of 11.2% (65/582) of pregnant women at 35–37 weeks of gestation were found to carry GBS. The sensitivity and specificity of L-S StrepB were 96.9% and 100%, respectively. Additionally, 87.3% (55/63) of GBS cases were detected within 15–24 h using L-S StrepB, demonstrating rapid processing time. This method is user-friendly, cost-effective, and requires no specialized equipment, making it suitable for use in primary healthcare settings.IMPORTANCEThe clinical application methods for group B streptococcus (GBS) detection among peripartum women in medical institutions in China are influenced by various factors, such as cost and personnel availability, leading to a lack of standardization. In this study, we evaluated a novel liquid-solid biphasic differential medium (L-S StrepB) that integrates liquid enrichment with solid agar for the detection of GBS. This is the first reported study in China to utilize biphasic medium for GBS detection. L-S StrepB demonstrates good sensitivity and specificity, with simple operations and low costs, while adhering to guidelines recommending initial enrichment followed by culturing. |
| format | Article |
| id | doaj-art-e49b124fec50475fbecd47a9d377550b |
| institution | DOAJ |
| issn | 2165-0497 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj-art-e49b124fec50475fbecd47a9d377550b2025-08-20T03:07:38ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972025-06-0113610.1128/spectrum.00140-25Evaluation of a novel liquid-solid biphasic differential medium for screening of group B streptococcus in perinatal womenShuo Chen0Fangqu Li1Xiangyang Li2Hao Chen3Department of Nosocomial Infection Prevention and Control, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, ChinaWenzhou Medical University, Wenzhou, Zhejiang Province, ChinaWenzhou Medical University, Wenzhou, Zhejiang Province, ChinaWenzhou Medical University, Wenzhou, Zhejiang Province, ChinaABSTRACT This study explored the capabilities of the commonly used blood agar plate in Chinese clinical microbiology laboratories and a novel liquid-solid biphasic differential medium (L-S StrepB) for detecting group B streptococcus (GBS) in peripartum women in China. A total of 11.2% (65/582) of pregnant women at 35–37 weeks of gestation were found to carry GBS. The sensitivity and specificity of L-S StrepB were 96.9% and 100%, respectively. Additionally, 87.3% (55/63) of GBS cases were detected within 15–24 h using L-S StrepB, demonstrating rapid processing time. This method is user-friendly, cost-effective, and requires no specialized equipment, making it suitable for use in primary healthcare settings.IMPORTANCEThe clinical application methods for group B streptococcus (GBS) detection among peripartum women in medical institutions in China are influenced by various factors, such as cost and personnel availability, leading to a lack of standardization. In this study, we evaluated a novel liquid-solid biphasic differential medium (L-S StrepB) that integrates liquid enrichment with solid agar for the detection of GBS. This is the first reported study in China to utilize biphasic medium for GBS detection. L-S StrepB demonstrates good sensitivity and specificity, with simple operations and low costs, while adhering to guidelines recommending initial enrichment followed by culturing.https://journals.asm.org/doi/10.1128/spectrum.00140-25group B streptococcusnovel liquid-solid biphasic differential mediumblood agar plate |
| spellingShingle | Shuo Chen Fangqu Li Xiangyang Li Hao Chen Evaluation of a novel liquid-solid biphasic differential medium for screening of group B streptococcus in perinatal women Microbiology Spectrum group B streptococcus novel liquid-solid biphasic differential medium blood agar plate |
| title | Evaluation of a novel liquid-solid biphasic differential medium for screening of group B streptococcus in perinatal women |
| title_full | Evaluation of a novel liquid-solid biphasic differential medium for screening of group B streptococcus in perinatal women |
| title_fullStr | Evaluation of a novel liquid-solid biphasic differential medium for screening of group B streptococcus in perinatal women |
| title_full_unstemmed | Evaluation of a novel liquid-solid biphasic differential medium for screening of group B streptococcus in perinatal women |
| title_short | Evaluation of a novel liquid-solid biphasic differential medium for screening of group B streptococcus in perinatal women |
| title_sort | evaluation of a novel liquid solid biphasic differential medium for screening of group b streptococcus in perinatal women |
| topic | group B streptococcus novel liquid-solid biphasic differential medium blood agar plate |
| url | https://journals.asm.org/doi/10.1128/spectrum.00140-25 |
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