Osteoblasts induce glucose-derived ATP perturbations in chondrocytes through noncontact communication
Cartilage and subchondral bone communicate with each other through material and signal exchanges. However, direct evidence provided by experimental studies on their interactions is insufficient. In the present study, we establish a noncontact co-culture model with a transwell chamber to explore the...
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| Format: | Article |
| Language: | English |
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China Science Publishing & Media Ltd.
2022-05-01
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| Series: | Acta Biochimica et Biophysica Sinica |
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| Online Access: | https://www.sciengine.com/doi/10.3724/abbs.2022042 |
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| author | Wei Jieya Yang Yueyi Guo Daimo Xu Siqun Huang Hongcan Zhang Demao Xie Jing Zhou Xuedong |
| author_facet | Wei Jieya Yang Yueyi Guo Daimo Xu Siqun Huang Hongcan Zhang Demao Xie Jing Zhou Xuedong |
| author_sort | Wei Jieya |
| collection | DOAJ |
| description | Cartilage and subchondral bone communicate with each other through material and signal exchanges. However, direct evidence provided by experimental studies on their interactions is insufficient. In the present study, we establish a noncontact co-culture model with a transwell chamber to explore the energetic perturbations in chondrocytes influenced by osteoblasts. Our results indicate that osteoblasts induce more ATP generation in chondrocytes through an energetic shift characterized by enhanced glycolysis and impaired mitochondrial tricarboxylic acid cycle. Enhanced glycolysis is shown by an increase of secreted lactate and the upregulation of glycolytic enzymes, including glucose-6-phosphate isomerase (Gpi), liver type ATP-dependent 6-phosphofructokinase (Pfkl), fructose-bisphosphate aldolase C (Aldoc), glyceraldehyde-3-phosphate dehydrogenase (Gapdh), triosephosphate isomerase (Tpi1), and phosphoglycerate kinase 1 (Pgk1). Impaired mitochondrial tricarboxylic acid cycle is characterized by the downregulation of cytoplasmic aspartate aminotransferase (Got1) and mitochondrial citrate synthase (Cs). Osteoblasts induce the activation of Akt and P38 signaling to mediate ATP perturbations in chondrocytes. This study may deepen our understanding of the maintenance of metabolic homeostasis in the bone-cartilage unit. |
| format | Article |
| id | doaj-art-e49302bc01a24b828c31a8e89279d36f |
| institution | OA Journals |
| issn | 1672-9145 |
| language | English |
| publishDate | 2022-05-01 |
| publisher | China Science Publishing & Media Ltd. |
| record_format | Article |
| series | Acta Biochimica et Biophysica Sinica |
| spelling | doaj-art-e49302bc01a24b828c31a8e89279d36f2025-08-20T01:52:10ZengChina Science Publishing & Media Ltd.Acta Biochimica et Biophysica Sinica1672-91452022-05-015462563610.3724/abbs.202204220d259ccOsteoblasts induce glucose-derived ATP perturbations in chondrocytes through noncontact communicationWei Jieya0Yang Yueyi1Guo Daimo2Xu Siqun3Huang Hongcan4Zhang Demao5Xie Jing6Zhou Xuedong7["State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China"]["State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China"]["State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China"]["State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China"]["State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China"]["State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China"]["State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China"]["State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China","Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China"]Cartilage and subchondral bone communicate with each other through material and signal exchanges. However, direct evidence provided by experimental studies on their interactions is insufficient. In the present study, we establish a noncontact co-culture model with a transwell chamber to explore the energetic perturbations in chondrocytes influenced by osteoblasts. Our results indicate that osteoblasts induce more ATP generation in chondrocytes through an energetic shift characterized by enhanced glycolysis and impaired mitochondrial tricarboxylic acid cycle. Enhanced glycolysis is shown by an increase of secreted lactate and the upregulation of glycolytic enzymes, including glucose-6-phosphate isomerase (Gpi), liver type ATP-dependent 6-phosphofructokinase (Pfkl), fructose-bisphosphate aldolase C (Aldoc), glyceraldehyde-3-phosphate dehydrogenase (Gapdh), triosephosphate isomerase (Tpi1), and phosphoglycerate kinase 1 (Pgk1). Impaired mitochondrial tricarboxylic acid cycle is characterized by the downregulation of cytoplasmic aspartate aminotransferase (Got1) and mitochondrial citrate synthase (Cs). Osteoblasts induce the activation of Akt and P38 signaling to mediate ATP perturbations in chondrocytes. This study may deepen our understanding of the maintenance of metabolic homeostasis in the bone-cartilage unit.https://www.sciengine.com/doi/10.3724/abbs.2022042adenosine triphosphatecarbon metabolismchondrocyteco-cultureosteoblast |
| spellingShingle | Wei Jieya Yang Yueyi Guo Daimo Xu Siqun Huang Hongcan Zhang Demao Xie Jing Zhou Xuedong Osteoblasts induce glucose-derived ATP perturbations in chondrocytes through noncontact communication Acta Biochimica et Biophysica Sinica adenosine triphosphate carbon metabolism chondrocyte co-culture osteoblast |
| title | Osteoblasts induce glucose-derived ATP perturbations in chondrocytes through noncontact communication |
| title_full | Osteoblasts induce glucose-derived ATP perturbations in chondrocytes through noncontact communication |
| title_fullStr | Osteoblasts induce glucose-derived ATP perturbations in chondrocytes through noncontact communication |
| title_full_unstemmed | Osteoblasts induce glucose-derived ATP perturbations in chondrocytes through noncontact communication |
| title_short | Osteoblasts induce glucose-derived ATP perturbations in chondrocytes through noncontact communication |
| title_sort | osteoblasts induce glucose derived atp perturbations in chondrocytes through noncontact communication |
| topic | adenosine triphosphate carbon metabolism chondrocyte co-culture osteoblast |
| url | https://www.sciengine.com/doi/10.3724/abbs.2022042 |
| work_keys_str_mv | AT weijieya osteoblastsinduceglucosederivedatpperturbationsinchondrocytesthroughnoncontactcommunication AT yangyueyi osteoblastsinduceglucosederivedatpperturbationsinchondrocytesthroughnoncontactcommunication AT guodaimo osteoblastsinduceglucosederivedatpperturbationsinchondrocytesthroughnoncontactcommunication AT xusiqun osteoblastsinduceglucosederivedatpperturbationsinchondrocytesthroughnoncontactcommunication AT huanghongcan osteoblastsinduceglucosederivedatpperturbationsinchondrocytesthroughnoncontactcommunication AT zhangdemao osteoblastsinduceglucosederivedatpperturbationsinchondrocytesthroughnoncontactcommunication AT xiejing osteoblastsinduceglucosederivedatpperturbationsinchondrocytesthroughnoncontactcommunication AT zhouxuedong osteoblastsinduceglucosederivedatpperturbationsinchondrocytesthroughnoncontactcommunication |