Antibacterial and biocompatibility properties of bioactive glass and double antibiotic-loaded hydrogels in regenerative endodontic treatment
Abstract This study evaluated the antibacterial and biocompatibility effects of Gelatin Methacrylate Hydrogel (GelMA) loaded with a double antibiotic paste (DAP) and bioactive glass (BG). The cellular impact of 1 mg/mL (1DAP) and 5 mg/mL (5DAP) DAP, as well as 1% BG (1BG) and 10% BG (10BG) within th...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-07-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-09057-0 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849335111740293120 |
|---|---|
| author | Meltem Kucuk Grace Quevedo Mohini Ratakonda Hacer Aksel |
| author_facet | Meltem Kucuk Grace Quevedo Mohini Ratakonda Hacer Aksel |
| author_sort | Meltem Kucuk |
| collection | DOAJ |
| description | Abstract This study evaluated the antibacterial and biocompatibility effects of Gelatin Methacrylate Hydrogel (GelMA) loaded with a double antibiotic paste (DAP) and bioactive glass (BG). The cellular impact of 1 mg/mL (1DAP) and 5 mg/mL (5DAP) DAP, as well as 1% BG (1BG) and 10% BG (10BG) within the hydrogel on dental pulp stem cells (DPSCs), was assessed using ATP cell viability and alkaline phosphatase (ALP) assays over 21 days. Antibacterial efficacy against E. faecalis was measured with and without dentin disks after 24-hour incubation, followed by colony-forming unit (CFU) counting. The statistical analysis was performed using one- and two-way ANOVA and Kruskal-Wallis tests. Results indicated that 5DAP and 10BG significantly reduced the vitality of DPSCs compared to 1DAP, 1BG, GelMA alone, and the control group (p < 0.001). Conversely, 1DAP and 1BG demonstrated the highest cell viability, with 1BG exhibiting superior ALP activity (p < 0.001). In the absence of dentin disks, 10BG and 5DAP displayed the greatest antibacterial effects (p < 0.001). However, the presence of dentin significantly diminished the antibacterial efficacy of BG, with 5DAP showing the strongest effect on infected dentin disks (p = 0.008). GelMA with 1% BG enhanced cellular functions. Dentin significantly reduced the antibacterial efficacy of BG, regardless of concentration. DAP at 5 mg/mL provided the highest antibacterial activity on infected dentin disks. However, none of the hydrogels completely eliminated bacteria from the dentin disks. |
| format | Article |
| id | doaj-art-e490d75227b54a75b3aba58e0a7e88ec |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-e490d75227b54a75b3aba58e0a7e88ec2025-08-20T03:45:23ZengNature PortfolioScientific Reports2045-23222025-07-011511810.1038/s41598-025-09057-0Antibacterial and biocompatibility properties of bioactive glass and double antibiotic-loaded hydrogels in regenerative endodontic treatmentMeltem Kucuk0Grace Quevedo1Mohini Ratakonda2Hacer Aksel3Department of Endodontics, Faculty of Dentistry, Near East UniversityDepartment of Periodontics and Endodontics, School of Dental Medicine, University at BuffaloDepartment of Periodontics and Endodontics, School of Dental Medicine, University at BuffaloDepartment of Periodontics and Endodontics, School of Dental Medicine, University at BuffaloAbstract This study evaluated the antibacterial and biocompatibility effects of Gelatin Methacrylate Hydrogel (GelMA) loaded with a double antibiotic paste (DAP) and bioactive glass (BG). The cellular impact of 1 mg/mL (1DAP) and 5 mg/mL (5DAP) DAP, as well as 1% BG (1BG) and 10% BG (10BG) within the hydrogel on dental pulp stem cells (DPSCs), was assessed using ATP cell viability and alkaline phosphatase (ALP) assays over 21 days. Antibacterial efficacy against E. faecalis was measured with and without dentin disks after 24-hour incubation, followed by colony-forming unit (CFU) counting. The statistical analysis was performed using one- and two-way ANOVA and Kruskal-Wallis tests. Results indicated that 5DAP and 10BG significantly reduced the vitality of DPSCs compared to 1DAP, 1BG, GelMA alone, and the control group (p < 0.001). Conversely, 1DAP and 1BG demonstrated the highest cell viability, with 1BG exhibiting superior ALP activity (p < 0.001). In the absence of dentin disks, 10BG and 5DAP displayed the greatest antibacterial effects (p < 0.001). However, the presence of dentin significantly diminished the antibacterial efficacy of BG, with 5DAP showing the strongest effect on infected dentin disks (p = 0.008). GelMA with 1% BG enhanced cellular functions. Dentin significantly reduced the antibacterial efficacy of BG, regardless of concentration. DAP at 5 mg/mL provided the highest antibacterial activity on infected dentin disks. However, none of the hydrogels completely eliminated bacteria from the dentin disks.https://doi.org/10.1038/s41598-025-09057-0Bioactive glassDouble antibiotic pasteHydrogelRegenerative endodontics |
| spellingShingle | Meltem Kucuk Grace Quevedo Mohini Ratakonda Hacer Aksel Antibacterial and biocompatibility properties of bioactive glass and double antibiotic-loaded hydrogels in regenerative endodontic treatment Scientific Reports Bioactive glass Double antibiotic paste Hydrogel Regenerative endodontics |
| title | Antibacterial and biocompatibility properties of bioactive glass and double antibiotic-loaded hydrogels in regenerative endodontic treatment |
| title_full | Antibacterial and biocompatibility properties of bioactive glass and double antibiotic-loaded hydrogels in regenerative endodontic treatment |
| title_fullStr | Antibacterial and biocompatibility properties of bioactive glass and double antibiotic-loaded hydrogels in regenerative endodontic treatment |
| title_full_unstemmed | Antibacterial and biocompatibility properties of bioactive glass and double antibiotic-loaded hydrogels in regenerative endodontic treatment |
| title_short | Antibacterial and biocompatibility properties of bioactive glass and double antibiotic-loaded hydrogels in regenerative endodontic treatment |
| title_sort | antibacterial and biocompatibility properties of bioactive glass and double antibiotic loaded hydrogels in regenerative endodontic treatment |
| topic | Bioactive glass Double antibiotic paste Hydrogel Regenerative endodontics |
| url | https://doi.org/10.1038/s41598-025-09057-0 |
| work_keys_str_mv | AT meltemkucuk antibacterialandbiocompatibilitypropertiesofbioactiveglassanddoubleantibioticloadedhydrogelsinregenerativeendodontictreatment AT gracequevedo antibacterialandbiocompatibilitypropertiesofbioactiveglassanddoubleantibioticloadedhydrogelsinregenerativeendodontictreatment AT mohiniratakonda antibacterialandbiocompatibilitypropertiesofbioactiveglassanddoubleantibioticloadedhydrogelsinregenerativeendodontictreatment AT haceraksel antibacterialandbiocompatibilitypropertiesofbioactiveglassanddoubleantibioticloadedhydrogelsinregenerativeendodontictreatment |