Bioinformatics Analysis and Functional Verification of Phytoene Synthase Gene <i>Pj</i>PSY1 of <i>Panax japonicus</i> C. A. Meyer
Phytoene synthase (PSY) is a multimeric enzyme that serves as the first enzyme in carotenoid synthesis within plant tissues and plays a crucial role in the production of carotenoids in plants. To understand the function of the PSY gene in <i>Panax japonicus</i> C. A. Meyer. fruit, the ge...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-07-01
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| Series: | Current Issues in Molecular Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1467-3045/47/7/551 |
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| Summary: | Phytoene synthase (PSY) is a multimeric enzyme that serves as the first enzyme in carotenoid synthesis within plant tissues and plays a crucial role in the production of carotenoids in plants. To understand the function of the PSY gene in <i>Panax japonicus</i> C. A. Meyer. fruit, the gene’s transcript was obtained by analyzing the transcriptome sequencing data of <i>Panax japonicus</i> fruit. The CDS sequence of the gene was cloned from <i>Panax japonicus</i> fruit using the RT-PCR cloning technique and named <i>Pj</i>PSY1, which was then subjected to biosynthetic analysis and functional verification. The results showed that the open reading frame of the gene was 1269 bp, encoding 423 amino acids, with a protein molecular mass of 47,654.67 KDa and an isoelectric point (pI) of 8.63; the protein encoded by these amino acids was hydrophilic and localized in chloroplasts, and its three-dimensional structure was predicted by combining the pymol software to annotate the N site of action and active centre of the protein. Phylogenetic analysis demonstrated that <i>Pj</i>PSY1 had the closest affinity to <i>Dc</i>PSY from <i>Daucus carota</i>. Overexpression of <i>Pj</i>PSY1 led to a significant increase in the content of carotenoid-related monomers in <i>Arabidopsis thaliana</i>, with Violaxanthin being synthesized in transgenic <i>Arabidopsis thaliana</i> but not in wild-type <i>Arabidopsis thaliana</i>. The <i>Pj</i>PSY1 clone obtained in this study was able to promote carotenoid synthesis in the fruits of <i>Panax japonicus</i>, revealing that the mode of action of <i>Pj</i>PSY1 in the carotenoid biosynthesis pathway of <i>Panax japonicus</i> fruits has a positive regulatory effect. |
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| ISSN: | 1467-3037 1467-3045 |