Antimicrobial Potential of Calotropis gigantea Leaf Against Klebsiella pneumoniae in Ventilator-Associated Pneumonia
Calotropis gigantea or biduri has traditional medicinal properties. However, the effect of C. gigantea leaves in assisting the function of antibiotic-resistant ventilator-associated pneumonia (VAP), particularly caused by Klebsiella pneumoniae, has not been evaluated. The purpose of this study was t...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Ital Publication
2024-09-01
|
| Series: | Journal of Human, Earth, and Future |
| Subjects: | |
| Online Access: | https://hefjournal.org/index.php/HEF/article/view/362 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850062598238633984 |
|---|---|
| author | Teuku Zulfikar Tongku N. Siregar Anna Rozaliyani Amalia Sutriana |
| author_facet | Teuku Zulfikar Tongku N. Siregar Anna Rozaliyani Amalia Sutriana |
| author_sort | Teuku Zulfikar |
| collection | DOAJ |
| description | Calotropis gigantea or biduri has traditional medicinal properties. However, the effect of C. gigantea leaves in assisting the function of antibiotic-resistant ventilator-associated pneumonia (VAP), particularly caused by Klebsiella pneumoniae, has not been evaluated. The purpose of this study was to identify the active compounds of C. gigantea leaf extracts growing in the coastal area of Alue Naga, Banda Aceh, Indonesia, and assess its antimicrobial potential in preventing microbial resistance. C. gigantea leaves were extracted using three different solvents: ethanol, ethyl acetate, and n-hexane. The three extracts of C. gigantea leaves were analyzed for antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, and the active compounds of C. gigantea were identified using gas chromatography-mass spectroscopy (GC-MS). The dominant bioactive compounds of the C. gigantea extract were enrolled for molecular docking analysis. The results of this study showed that the inhibitory concentration 50% (IC50) of the ethanol extract had a higher antioxidant activity (IC50 value of 3.3 ppm) than the ethyl acetate (IC50 value of 22.97 ppm) and n-hexane (IC50 value of 32.9 ppm). Bioactive compound identification using GC-MS from the three extracts showed similar dominant compounds, which were α-amyrin and lup-20(29)-en-3-ol, and these compounds belonged to the class of triterpenoid derivative compounds. Molecular docking analysis showed that α-amyrin (-9.6 Kcal/mol), β-amyrin (-9.6 Kcal/mol), and epilupeol (-9.2 Kcal/mol) in C. gigantea leaves had higher binding free energy values compared to cefixime (-8.7 Kcal/mol). Thus, it could be concluded that C. gigantea leaf extract is assumed to have great potential as an antimicrobial agent and in preventing microbial resistance, particularly in cases of VAP caused by Klebsiella pneumoniae.
Doi: 10.28991/HEF-2024-05-03-010
Full Text: PDF |
| format | Article |
| id | doaj-art-e47be659eb97425f8adb39bc484a4e1a |
| institution | DOAJ |
| issn | 2785-2997 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Ital Publication |
| record_format | Article |
| series | Journal of Human, Earth, and Future |
| spelling | doaj-art-e47be659eb97425f8adb39bc484a4e1a2025-08-20T02:49:52ZengItal PublicationJournal of Human, Earth, and Future2785-29972024-09-015345647010.28991/HEF-2024-05-03-010155Antimicrobial Potential of Calotropis gigantea Leaf Against Klebsiella pneumoniae in Ventilator-Associated PneumoniaTeuku Zulfikar0Tongku N. Siregar1Anna Rozaliyani2Amalia Sutriana3Graduate School of Mathematics and Applied Science, Universitas Syiah Kuala, Banda Aceh,Laboratory of Reproduction, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh,Department of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta,Laboratory of Pharmacology, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh,Calotropis gigantea or biduri has traditional medicinal properties. However, the effect of C. gigantea leaves in assisting the function of antibiotic-resistant ventilator-associated pneumonia (VAP), particularly caused by Klebsiella pneumoniae, has not been evaluated. The purpose of this study was to identify the active compounds of C. gigantea leaf extracts growing in the coastal area of Alue Naga, Banda Aceh, Indonesia, and assess its antimicrobial potential in preventing microbial resistance. C. gigantea leaves were extracted using three different solvents: ethanol, ethyl acetate, and n-hexane. The three extracts of C. gigantea leaves were analyzed for antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, and the active compounds of C. gigantea were identified using gas chromatography-mass spectroscopy (GC-MS). The dominant bioactive compounds of the C. gigantea extract were enrolled for molecular docking analysis. The results of this study showed that the inhibitory concentration 50% (IC50) of the ethanol extract had a higher antioxidant activity (IC50 value of 3.3 ppm) than the ethyl acetate (IC50 value of 22.97 ppm) and n-hexane (IC50 value of 32.9 ppm). Bioactive compound identification using GC-MS from the three extracts showed similar dominant compounds, which were α-amyrin and lup-20(29)-en-3-ol, and these compounds belonged to the class of triterpenoid derivative compounds. Molecular docking analysis showed that α-amyrin (-9.6 Kcal/mol), β-amyrin (-9.6 Kcal/mol), and epilupeol (-9.2 Kcal/mol) in C. gigantea leaves had higher binding free energy values compared to cefixime (-8.7 Kcal/mol). Thus, it could be concluded that C. gigantea leaf extract is assumed to have great potential as an antimicrobial agent and in preventing microbial resistance, particularly in cases of VAP caused by Klebsiella pneumoniae. Doi: 10.28991/HEF-2024-05-03-010 Full Text: PDFhttps://hefjournal.org/index.php/HEF/article/view/362calotropis giganteabiduriventilator-associated pneumonia (vap)α-amyrinalue naga. |
| spellingShingle | Teuku Zulfikar Tongku N. Siregar Anna Rozaliyani Amalia Sutriana Antimicrobial Potential of Calotropis gigantea Leaf Against Klebsiella pneumoniae in Ventilator-Associated Pneumonia Journal of Human, Earth, and Future calotropis gigantea biduri ventilator-associated pneumonia (vap) α-amyrin alue naga. |
| title | Antimicrobial Potential of Calotropis gigantea Leaf Against Klebsiella pneumoniae in Ventilator-Associated Pneumonia |
| title_full | Antimicrobial Potential of Calotropis gigantea Leaf Against Klebsiella pneumoniae in Ventilator-Associated Pneumonia |
| title_fullStr | Antimicrobial Potential of Calotropis gigantea Leaf Against Klebsiella pneumoniae in Ventilator-Associated Pneumonia |
| title_full_unstemmed | Antimicrobial Potential of Calotropis gigantea Leaf Against Klebsiella pneumoniae in Ventilator-Associated Pneumonia |
| title_short | Antimicrobial Potential of Calotropis gigantea Leaf Against Klebsiella pneumoniae in Ventilator-Associated Pneumonia |
| title_sort | antimicrobial potential of calotropis gigantea leaf against klebsiella pneumoniae in ventilator associated pneumonia |
| topic | calotropis gigantea biduri ventilator-associated pneumonia (vap) α-amyrin alue naga. |
| url | https://hefjournal.org/index.php/HEF/article/view/362 |
| work_keys_str_mv | AT teukuzulfikar antimicrobialpotentialofcalotropisgigantealeafagainstklebsiellapneumoniaeinventilatorassociatedpneumonia AT tongkunsiregar antimicrobialpotentialofcalotropisgigantealeafagainstklebsiellapneumoniaeinventilatorassociatedpneumonia AT annarozaliyani antimicrobialpotentialofcalotropisgigantealeafagainstklebsiellapneumoniaeinventilatorassociatedpneumonia AT amaliasutriana antimicrobialpotentialofcalotropisgigantealeafagainstklebsiellapneumoniaeinventilatorassociatedpneumonia |