Phenotypic Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing <i>Enterobacteriaceae</i> from Wastewater Treatment Plants in Ouagadougou, Burkina Faso
<b>Background/Objectives:</b> The overuse of antibiotics has led to the emergence of antibiotic-resistant bacteria, posing a global public health challenge. Among these, extended-spectrum β-lactamase and carbapenemase-producing <i>Enterobacteriaceae</i> (ESBL-E and CPE) are o...
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MDPI AG
2025-06-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/14/7/641 |
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| author | Inayatou Hamma Yacouba Yacouba Konaté Bapio Valérie Elvira Jean Télesphore Bazie Boukary Sawadogo |
| author_facet | Inayatou Hamma Yacouba Yacouba Konaté Bapio Valérie Elvira Jean Télesphore Bazie Boukary Sawadogo |
| author_sort | Inayatou Hamma Yacouba |
| collection | DOAJ |
| description | <b>Background/Objectives:</b> The overuse of antibiotics has led to the emergence of antibiotic-resistant bacteria, posing a global public health challenge. Among these, extended-spectrum β-lactamase and carbapenemase-producing <i>Enterobacteriaceae</i> (ESBL-E and CPE) are of particular concern due to their potential to spread resistance in various environments. Understanding the prevalence and spread of these bacteria in the influent and effluent of wastewater treatment plants is essential. <b>Methods:</b> This study examined ESBL-E and CPE in wastewater from three WWTPs in Ouagadougou, Burkina Faso, and was conducted between February and August 2024. Phenotypic detection of ESBL-E was performed on the isolates using the double-disk synergy test and the combination disk test, whereas the CPE detection employed the combination disk test and the modified Hodge test. <b>Results:</b> A total of 250 <i>Enterobacteriaceae</i> isolates were found, with <i>Escherichia coli</i>, <i>Klebsiella</i> spp., <i>Enterobacter</i> spp., and <i>Buttiauxella</i> spp. the most predominant taxa. Phenotypic analysis revealed a high prevalence of ESBL-E, particularly in influent samples, with rates ranging from 55 to 98% across the WWTPs. CPE detection showed varying prevalence, with higher proportions identified in effluent samples, ranging from 37 to 68%, depending on the plant. These findings highlight the critical role of WWTPs in the persistence and potential spread of antibiotic-resistant bacteria. <b>Conclusions:</b> This study underscores the urgent need for improved wastewater treatment technologies and comprehensive monitoring systems to reduce the dissemination of ESBL-E and CPE in the environment. Addressing these challenges is crucial for mitigating the public health risks associated with antimicrobial resistance. |
| format | Article |
| id | doaj-art-e47affd56dad4cb8ac6cb295a98760cc |
| institution | DOAJ |
| issn | 2079-6382 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj-art-e47affd56dad4cb8ac6cb295a98760cc2025-08-20T02:45:52ZengMDPI AGAntibiotics2079-63822025-06-0114764110.3390/antibiotics14070641Phenotypic Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing <i>Enterobacteriaceae</i> from Wastewater Treatment Plants in Ouagadougou, Burkina FasoInayatou Hamma Yacouba0Yacouba Konaté1Bapio Valérie Elvira Jean Télesphore Bazie2Boukary Sawadogo3Laboratoire Eaux, Hydro-Systèmes et Agriculture (LEHSA), Institut International d’Ingénierie de l’Eau et de L’Environnement (2iE), Ouagadougou 01 BP 594, Burkina FasoLaboratoire Eaux, Hydro-Systèmes et Agriculture (LEHSA), Institut International d’Ingénierie de l’Eau et de L’Environnement (2iE), Ouagadougou 01 BP 594, Burkina FasoCentre National de la Recherche Scientifique et Technologique (CNRST), Institut de Recherche en Sciences de la Santé (IRSS), Ouagadougou 03 BP 7047, Burkina FasoLaboratoire Eaux, Hydro-Systèmes et Agriculture (LEHSA), Institut International d’Ingénierie de l’Eau et de L’Environnement (2iE), Ouagadougou 01 BP 594, Burkina Faso<b>Background/Objectives:</b> The overuse of antibiotics has led to the emergence of antibiotic-resistant bacteria, posing a global public health challenge. Among these, extended-spectrum β-lactamase and carbapenemase-producing <i>Enterobacteriaceae</i> (ESBL-E and CPE) are of particular concern due to their potential to spread resistance in various environments. Understanding the prevalence and spread of these bacteria in the influent and effluent of wastewater treatment plants is essential. <b>Methods:</b> This study examined ESBL-E and CPE in wastewater from three WWTPs in Ouagadougou, Burkina Faso, and was conducted between February and August 2024. Phenotypic detection of ESBL-E was performed on the isolates using the double-disk synergy test and the combination disk test, whereas the CPE detection employed the combination disk test and the modified Hodge test. <b>Results:</b> A total of 250 <i>Enterobacteriaceae</i> isolates were found, with <i>Escherichia coli</i>, <i>Klebsiella</i> spp., <i>Enterobacter</i> spp., and <i>Buttiauxella</i> spp. the most predominant taxa. Phenotypic analysis revealed a high prevalence of ESBL-E, particularly in influent samples, with rates ranging from 55 to 98% across the WWTPs. CPE detection showed varying prevalence, with higher proportions identified in effluent samples, ranging from 37 to 68%, depending on the plant. These findings highlight the critical role of WWTPs in the persistence and potential spread of antibiotic-resistant bacteria. <b>Conclusions:</b> This study underscores the urgent need for improved wastewater treatment technologies and comprehensive monitoring systems to reduce the dissemination of ESBL-E and CPE in the environment. Addressing these challenges is crucial for mitigating the public health risks associated with antimicrobial resistance.https://www.mdpi.com/2079-6382/14/7/641antibiotic-resistant bacteriaESBL-ECPEwastewater treatment plantsBurkina Faso |
| spellingShingle | Inayatou Hamma Yacouba Yacouba Konaté Bapio Valérie Elvira Jean Télesphore Bazie Boukary Sawadogo Phenotypic Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing <i>Enterobacteriaceae</i> from Wastewater Treatment Plants in Ouagadougou, Burkina Faso Antibiotics antibiotic-resistant bacteria ESBL-E CPE wastewater treatment plants Burkina Faso |
| title | Phenotypic Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing <i>Enterobacteriaceae</i> from Wastewater Treatment Plants in Ouagadougou, Burkina Faso |
| title_full | Phenotypic Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing <i>Enterobacteriaceae</i> from Wastewater Treatment Plants in Ouagadougou, Burkina Faso |
| title_fullStr | Phenotypic Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing <i>Enterobacteriaceae</i> from Wastewater Treatment Plants in Ouagadougou, Burkina Faso |
| title_full_unstemmed | Phenotypic Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing <i>Enterobacteriaceae</i> from Wastewater Treatment Plants in Ouagadougou, Burkina Faso |
| title_short | Phenotypic Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing <i>Enterobacteriaceae</i> from Wastewater Treatment Plants in Ouagadougou, Burkina Faso |
| title_sort | phenotypic detection of extended spectrum β lactamase and carbapenemase producing i enterobacteriaceae i from wastewater treatment plants in ouagadougou burkina faso |
| topic | antibiotic-resistant bacteria ESBL-E CPE wastewater treatment plants Burkina Faso |
| url | https://www.mdpi.com/2079-6382/14/7/641 |
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