Optimizing DNA extraction methods for enhanced detection of pathogenic Leptospira spp. in cultures using PCR

Optimizing DNA extraction methods for enhanced detection of pathogenic Leptospira spp. in cultures using PCR

Leptospirosis is a worldwide zoonosis caused by Leptospira interrogans. Laboratory diagnosis of leptospirosis can be done through sample culture. However, microscopic observation of positive Leptospira spp. culture need to be supported by molecular detection to confirm the presence of pathogenic Lep...

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Bibliographic Details
Main Authors: Kiki Aprilia, Arif Mulyanto, Kurnia Ritma Dhanti, Korri El Khobar, Farida Dwi Handayani
Format: Article
Language:Indonesian
Published: Poltekkes Kemenkes Yogyakarta 2024-12-01
Series:Jurnal Teknologi Laboratorium
Subjects:
dna concentration
dna extraction
dna purity
leptospira detection
pcr
Online Access:https://teknolabjournal.com/index.php/Jtl/article/view/480
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