Proteomic and metabolomic profiling of plasma uncovers immune responses in patients with Long COVID-19
Long COVID is an often-debilitating condition with severe, multisystem symptoms that can persist for weeks or months and increase the risk of various diseases. Currently, there is a lack of diagnostic tools for Long COVID in clinical practice. Therefore, this study utilizes plasma proteomics and met...
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1470193/full |
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| author | Yulin Wei Hongyan Gu Jun Ma Xiaojuan Mao Bing Wang Bing Wang Weiyan Wu Weiyan Wu Shiming Yu Shiming Yu Jinyuan Wang Jinyuan Wang Huan Zhao Yanbin He Yanbin He |
| author_facet | Yulin Wei Hongyan Gu Jun Ma Xiaojuan Mao Bing Wang Bing Wang Weiyan Wu Weiyan Wu Shiming Yu Shiming Yu Jinyuan Wang Jinyuan Wang Huan Zhao Yanbin He Yanbin He |
| author_sort | Yulin Wei |
| collection | DOAJ |
| description | Long COVID is an often-debilitating condition with severe, multisystem symptoms that can persist for weeks or months and increase the risk of various diseases. Currently, there is a lack of diagnostic tools for Long COVID in clinical practice. Therefore, this study utilizes plasma proteomics and metabolomics technologies to understand the molecular profile and pathophysiological mechanisms of Long COVID, providing clinical evidence for the development of potential biomarkers. This study included three age- and gender-matched cohorts: healthy controls (n = 18), COVID-19 recovered patients (n = 17), and Long COVID patients (n = 15). The proteomics results revealed significant differences in proteins between Long COVID-19 patients and COVID-19 recovered patients, with dysregulation mainly focused on pathways such as coagulation, platelets, complement cascade reactions, GPCR cell signal transduction, and substance transport, which can participate in regulating immune responses, inflammation, and tissue vascular repair. Metabolomics results showed that Long COVID patients and COVID-19 recovered patients have similar metabolic disorders, mainly involving dysregulation in lipid metabolites and fatty acid metabolism, such as glycerophospholipids, sphingolipid metabolism, and arachidonic acid metabolism processes. In summary, our study results indicate significant protein dysregulation and metabolic abnormalities in the plasma of Long COVID patients, leading to coagulation dysfunction, impaired energy metabolism, and chronic immune dysregulation, which are more pronounced than in COVID-19 recovered patients. |
| format | Article |
| id | doaj-art-e46ec36c863449a28f7a8f6ff198ec39 |
| institution | DOAJ |
| issn | 1664-302X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-e46ec36c863449a28f7a8f6ff198ec392025-08-20T02:43:28ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-12-011510.3389/fmicb.2024.14701931470193Proteomic and metabolomic profiling of plasma uncovers immune responses in patients with Long COVID-19Yulin Wei0Hongyan Gu1Jun Ma2Xiaojuan Mao3Bing Wang4Bing Wang5Weiyan Wu6Weiyan Wu7Shiming Yu8Shiming Yu9Jinyuan Wang10Jinyuan Wang11Huan Zhao12Yanbin He13Yanbin He14Department of Pulmonary and Critical Care Medicine, Affiliated Nantong Hospital of Shanghai University (The Sixth People’s Hospital of Nantong), Nantong, Jiangsu, ChinaDepartment of Pulmonary and Critical Care Medicine, Affiliated Nantong Hospital of Shanghai University (The Sixth People’s Hospital of Nantong), Nantong, Jiangsu, ChinaDepartment of Pulmonary and Critical Care Medicine, Affiliated Nantong Hospital of Shanghai University (The Sixth People’s Hospital of Nantong), Nantong, Jiangsu, ChinaDepartment of Pulmonary and Critical Care Medicine, Affiliated Nantong Hospital of Shanghai University (The Sixth People’s Hospital of Nantong), Nantong, Jiangsu, ChinaKey Laboratory of Digital Technology in Medical Diagnostics of Zhejiang Province, Hangzhou, ChinaDian Diagnostics Group Co., Ltd., Hangzhou, Zhejiang, ChinaKey Laboratory of Digital Technology in Medical Diagnostics of Zhejiang Province, Hangzhou, ChinaDian Diagnostics Group Co., Ltd., Hangzhou, Zhejiang, ChinaKey Laboratory of Digital Technology in Medical Diagnostics of Zhejiang Province, Hangzhou, ChinaDian Diagnostics Group Co., Ltd., Hangzhou, Zhejiang, ChinaKey Laboratory of Digital Technology in Medical Diagnostics of Zhejiang Province, Hangzhou, ChinaDian Diagnostics Group Co., Ltd., Hangzhou, Zhejiang, ChinaDepartment of Pulmonary and Critical Care Medicine, Affiliated Nantong Hospital of Shanghai University (The Sixth People’s Hospital of Nantong), Nantong, Jiangsu, ChinaKey Laboratory of Digital Technology in Medical Diagnostics of Zhejiang Province, Hangzhou, ChinaDian Diagnostics Group Co., Ltd., Hangzhou, Zhejiang, ChinaLong COVID is an often-debilitating condition with severe, multisystem symptoms that can persist for weeks or months and increase the risk of various diseases. Currently, there is a lack of diagnostic tools for Long COVID in clinical practice. Therefore, this study utilizes plasma proteomics and metabolomics technologies to understand the molecular profile and pathophysiological mechanisms of Long COVID, providing clinical evidence for the development of potential biomarkers. This study included three age- and gender-matched cohorts: healthy controls (n = 18), COVID-19 recovered patients (n = 17), and Long COVID patients (n = 15). The proteomics results revealed significant differences in proteins between Long COVID-19 patients and COVID-19 recovered patients, with dysregulation mainly focused on pathways such as coagulation, platelets, complement cascade reactions, GPCR cell signal transduction, and substance transport, which can participate in regulating immune responses, inflammation, and tissue vascular repair. Metabolomics results showed that Long COVID patients and COVID-19 recovered patients have similar metabolic disorders, mainly involving dysregulation in lipid metabolites and fatty acid metabolism, such as glycerophospholipids, sphingolipid metabolism, and arachidonic acid metabolism processes. In summary, our study results indicate significant protein dysregulation and metabolic abnormalities in the plasma of Long COVID patients, leading to coagulation dysfunction, impaired energy metabolism, and chronic immune dysregulation, which are more pronounced than in COVID-19 recovered patients.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1470193/fullLong COVIDmechanismproteomicsmetabolomicsplasma |
| spellingShingle | Yulin Wei Hongyan Gu Jun Ma Xiaojuan Mao Bing Wang Bing Wang Weiyan Wu Weiyan Wu Shiming Yu Shiming Yu Jinyuan Wang Jinyuan Wang Huan Zhao Yanbin He Yanbin He Proteomic and metabolomic profiling of plasma uncovers immune responses in patients with Long COVID-19 Frontiers in Microbiology Long COVID mechanism proteomics metabolomics plasma |
| title | Proteomic and metabolomic profiling of plasma uncovers immune responses in patients with Long COVID-19 |
| title_full | Proteomic and metabolomic profiling of plasma uncovers immune responses in patients with Long COVID-19 |
| title_fullStr | Proteomic and metabolomic profiling of plasma uncovers immune responses in patients with Long COVID-19 |
| title_full_unstemmed | Proteomic and metabolomic profiling of plasma uncovers immune responses in patients with Long COVID-19 |
| title_short | Proteomic and metabolomic profiling of plasma uncovers immune responses in patients with Long COVID-19 |
| title_sort | proteomic and metabolomic profiling of plasma uncovers immune responses in patients with long covid 19 |
| topic | Long COVID mechanism proteomics metabolomics plasma |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1470193/full |
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