Discovery of differentially expressed lncRNAs in porcine ovaries with smaller and bigger litter size
IntroductionThe number of litters is an important reproductive trait, which is one of the main indicators reflecting the production level and economic benefit of the pig farm. As an important reproductive organ of female mammals, the ovary is controlled by a complex transcription network of coding a...
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Frontiers Media S.A.
2025-04-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2025.1498076/full |
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| author | Saixing Duan Falei Li Libing Meng Shimei Cheng Huangqi Shi Yingjie Qu Chaoyue He Shengyu Gao Jian Li Shiduo Sun Yong Liu Gaoxiao Xu Gaoxiao Xu |
| author_facet | Saixing Duan Falei Li Libing Meng Shimei Cheng Huangqi Shi Yingjie Qu Chaoyue He Shengyu Gao Jian Li Shiduo Sun Yong Liu Gaoxiao Xu Gaoxiao Xu |
| author_sort | Saixing Duan |
| collection | DOAJ |
| description | IntroductionThe number of litters is an important reproductive trait, which is one of the main indicators reflecting the production level and economic benefit of the pig farm. As an important reproductive organ of female mammals, the ovary is controlled by a complex transcription network of coding and non-coding genes to undergo a series of biological processes during each estrus cycle, thereby regulating the reproductive capacity of the sow. However, these molecular regulation mechanisms affecting sow litter size are still unclear.MethodsRegarding the non-coding molecular regulatory mechanisms in ovarian function with smaller and larger litter size (SLS and LLS), we investigated the expression profile of lncRNA in pig SLS and LLS samples. Total RNAs from porcine ovaries were used to construct libraries using Ribo-Zero RNA sequencing method.ResultsHere we profiled the expression of lncRNA in porcine ovaries with SLS and LLS, and identified a total of 3,556 lncRNA candidates, of which 96 were upregulated lncRNA and 206 were downregulated lncRNA when comparing LLS to SLS. In addition, a competitive endogenous RNA (ceRNA) network was constructed, and it was found that lncRNAs LOC100513133 and LOC102168075 may serve as ceRNAs containing potential binding sites for miR-26b, let-7g and miR-125b.DiscussionThese results demonstrate that lncRNAs may play roles in modulating porcine litter size. |
| format | Article |
| id | doaj-art-e46d6b3e812e4782841750d7bb0e32b1 |
| institution | DOAJ |
| issn | 1664-8021 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj-art-e46d6b3e812e4782841750d7bb0e32b12025-08-20T03:10:21ZengFrontiers Media S.A.Frontiers in Genetics1664-80212025-04-011610.3389/fgene.2025.14980761498076Discovery of differentially expressed lncRNAs in porcine ovaries with smaller and bigger litter sizeSaixing Duan0Falei Li1Libing Meng2Shimei Cheng3Huangqi Shi4Yingjie Qu5Chaoyue He6Shengyu Gao7Jian Li8Shiduo Sun9Yong Liu10Gaoxiao Xu11Gaoxiao Xu12Anhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Anhui Province Key Laboratory of Environmental Hormone and Reproduction, School of Biological and Food Engineering, Fuyang Normal University, Fuyang, ChinaAnhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Anhui Province Key Laboratory of Environmental Hormone and Reproduction, School of Biological and Food Engineering, Fuyang Normal University, Fuyang, ChinaAnhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Anhui Province Key Laboratory of Environmental Hormone and Reproduction, School of Biological and Food Engineering, Fuyang Normal University, Fuyang, ChinaAnhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Anhui Province Key Laboratory of Environmental Hormone and Reproduction, School of Biological and Food Engineering, Fuyang Normal University, Fuyang, ChinaAnhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Anhui Province Key Laboratory of Environmental Hormone and Reproduction, School of Biological and Food Engineering, Fuyang Normal University, Fuyang, ChinaAnhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Anhui Province Key Laboratory of Environmental Hormone and Reproduction, School of Biological and Food Engineering, Fuyang Normal University, Fuyang, ChinaAnhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Anhui Province Key Laboratory of Environmental Hormone and Reproduction, School of Biological and Food Engineering, Fuyang Normal University, Fuyang, ChinaAnhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Anhui Province Key Laboratory of Environmental Hormone and Reproduction, School of Biological and Food Engineering, Fuyang Normal University, Fuyang, ChinaAnhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Anhui Province Key Laboratory of Environmental Hormone and Reproduction, School of Biological and Food Engineering, Fuyang Normal University, Fuyang, ChinaShaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, ChinaAnhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Anhui Province Key Laboratory of Environmental Hormone and Reproduction, School of Biological and Food Engineering, Fuyang Normal University, Fuyang, ChinaAnhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Anhui Province Key Laboratory of Environmental Hormone and Reproduction, School of Biological and Food Engineering, Fuyang Normal University, Fuyang, ChinaShaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, ChinaIntroductionThe number of litters is an important reproductive trait, which is one of the main indicators reflecting the production level and economic benefit of the pig farm. As an important reproductive organ of female mammals, the ovary is controlled by a complex transcription network of coding and non-coding genes to undergo a series of biological processes during each estrus cycle, thereby regulating the reproductive capacity of the sow. However, these molecular regulation mechanisms affecting sow litter size are still unclear.MethodsRegarding the non-coding molecular regulatory mechanisms in ovarian function with smaller and larger litter size (SLS and LLS), we investigated the expression profile of lncRNA in pig SLS and LLS samples. Total RNAs from porcine ovaries were used to construct libraries using Ribo-Zero RNA sequencing method.ResultsHere we profiled the expression of lncRNA in porcine ovaries with SLS and LLS, and identified a total of 3,556 lncRNA candidates, of which 96 were upregulated lncRNA and 206 were downregulated lncRNA when comparing LLS to SLS. In addition, a competitive endogenous RNA (ceRNA) network was constructed, and it was found that lncRNAs LOC100513133 and LOC102168075 may serve as ceRNAs containing potential binding sites for miR-26b, let-7g and miR-125b.DiscussionThese results demonstrate that lncRNAs may play roles in modulating porcine litter size.https://www.frontiersin.org/articles/10.3389/fgene.2025.1498076/fulllncRNApigovarylitter sizeceRNA |
| spellingShingle | Saixing Duan Falei Li Libing Meng Shimei Cheng Huangqi Shi Yingjie Qu Chaoyue He Shengyu Gao Jian Li Shiduo Sun Yong Liu Gaoxiao Xu Gaoxiao Xu Discovery of differentially expressed lncRNAs in porcine ovaries with smaller and bigger litter size Frontiers in Genetics lncRNA pig ovary litter size ceRNA |
| title | Discovery of differentially expressed lncRNAs in porcine ovaries with smaller and bigger litter size |
| title_full | Discovery of differentially expressed lncRNAs in porcine ovaries with smaller and bigger litter size |
| title_fullStr | Discovery of differentially expressed lncRNAs in porcine ovaries with smaller and bigger litter size |
| title_full_unstemmed | Discovery of differentially expressed lncRNAs in porcine ovaries with smaller and bigger litter size |
| title_short | Discovery of differentially expressed lncRNAs in porcine ovaries with smaller and bigger litter size |
| title_sort | discovery of differentially expressed lncrnas in porcine ovaries with smaller and bigger litter size |
| topic | lncRNA pig ovary litter size ceRNA |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2025.1498076/full |
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