Remarkable increase in Interleukin-5 receptor expression beyond tissue eosinophils in inflammatory bowel disease
IntroductionThe role of tissue eosinophils and their relationship with interleukin-5 in gastrointestinal disorders have not been elucidated. This study aimed to analyze tissue eosinophils and interleukin-5 receptor-α subunit (IL-5RA) expression on eosinophils in inflammatory bowel disease (IBD).Meth...
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Frontiers Media S.A.
2025-05-01
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| author | You Ie Kim You Ie Kim Hyeon Jeong Oh Hye Ran Yang Hye Ran Yang |
| author_facet | You Ie Kim You Ie Kim Hyeon Jeong Oh Hye Ran Yang Hye Ran Yang |
| author_sort | You Ie Kim |
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| description | IntroductionThe role of tissue eosinophils and their relationship with interleukin-5 in gastrointestinal disorders have not been elucidated. This study aimed to analyze tissue eosinophils and interleukin-5 receptor-α subunit (IL-5RA) expression on eosinophils in inflammatory bowel disease (IBD).MethodsBlood and stool test results and radiologic and endoscopic findings in pediatric patients, who were newly diagnosed with IBD, eosinophilic gastrointestinal diseases (EGIDs), or disorders of gut–brain interactions were retrospectively reviewed. Tissue eosinophil counts were analyzed, and immunohistochemistry was performed using an antibody against IL-5RA expressed on eosinophils in the colonic mucosa.ResultsThe tissue eosinophil count and IL-5RA-expressing eosinophil density were significantly different among the three groups (P<0.005). IL-5RA expression was significantly higher in the IBD group than in the EGID group (P<0.001). In IBD, tissue IL-5RA expression was positively correlated with platelet count, erythrocyte sedimentation rate, and highly sensitive C-reactive protein, and negatively correlated with hemoglobin and albumin. In Crohn’s disease (CD), higher IL-5RA expressions were observed with higher pediatric CD activity index (P<0.005). The optimal cut-off value for IL-5RA-expressing eosinophil density was 69.4/high-power field.DiscussionThe expression of IL-5RA in tissue eosinophils is elevated in treatment naïve IBD. IL-5RA expression may reflect disease severity and its extent of involvement in early active CD. |
| format | Article |
| id | doaj-art-e46bfbe4075841aa85808a5881588a9d |
| institution | OA Journals |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
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| spelling | doaj-art-e46bfbe4075841aa85808a5881588a9d2025-08-20T02:25:40ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-05-011610.3389/fimmu.2025.15894211589421Remarkable increase in Interleukin-5 receptor expression beyond tissue eosinophils in inflammatory bowel diseaseYou Ie Kim0You Ie Kim1Hyeon Jeong Oh2Hye Ran Yang3Hye Ran Yang4Department of Pediatrics, Seoul National University College of Medicine, Seoul, Republic of KoreaDepartment of Pediatrics, Incheon St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of KoreaDepartment of Pathology, Seoul National University Bundang Hospital, Seongnam, Republic of KoreaDepartment of Pediatrics, Seoul National University College of Medicine, Seoul, Republic of KoreaDepartment of Pediatrics, Seoul National University Bundang Hospital, Seongnam, Republic of KoreaIntroductionThe role of tissue eosinophils and their relationship with interleukin-5 in gastrointestinal disorders have not been elucidated. This study aimed to analyze tissue eosinophils and interleukin-5 receptor-α subunit (IL-5RA) expression on eosinophils in inflammatory bowel disease (IBD).MethodsBlood and stool test results and radiologic and endoscopic findings in pediatric patients, who were newly diagnosed with IBD, eosinophilic gastrointestinal diseases (EGIDs), or disorders of gut–brain interactions were retrospectively reviewed. Tissue eosinophil counts were analyzed, and immunohistochemistry was performed using an antibody against IL-5RA expressed on eosinophils in the colonic mucosa.ResultsThe tissue eosinophil count and IL-5RA-expressing eosinophil density were significantly different among the three groups (P<0.005). IL-5RA expression was significantly higher in the IBD group than in the EGID group (P<0.001). In IBD, tissue IL-5RA expression was positively correlated with platelet count, erythrocyte sedimentation rate, and highly sensitive C-reactive protein, and negatively correlated with hemoglobin and albumin. In Crohn’s disease (CD), higher IL-5RA expressions were observed with higher pediatric CD activity index (P<0.005). The optimal cut-off value for IL-5RA-expressing eosinophil density was 69.4/high-power field.DiscussionThe expression of IL-5RA in tissue eosinophils is elevated in treatment naïve IBD. IL-5RA expression may reflect disease severity and its extent of involvement in early active CD.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1589421/fulleosinophilinterleukin-5 receptorinflammatory bowel diseasepathogenesisproinflammatory cytokine |
| spellingShingle | You Ie Kim You Ie Kim Hyeon Jeong Oh Hye Ran Yang Hye Ran Yang Remarkable increase in Interleukin-5 receptor expression beyond tissue eosinophils in inflammatory bowel disease Frontiers in Immunology eosinophil interleukin-5 receptor inflammatory bowel disease pathogenesis proinflammatory cytokine |
| title | Remarkable increase in Interleukin-5 receptor expression beyond tissue eosinophils in inflammatory bowel disease |
| title_full | Remarkable increase in Interleukin-5 receptor expression beyond tissue eosinophils in inflammatory bowel disease |
| title_fullStr | Remarkable increase in Interleukin-5 receptor expression beyond tissue eosinophils in inflammatory bowel disease |
| title_full_unstemmed | Remarkable increase in Interleukin-5 receptor expression beyond tissue eosinophils in inflammatory bowel disease |
| title_short | Remarkable increase in Interleukin-5 receptor expression beyond tissue eosinophils in inflammatory bowel disease |
| title_sort | remarkable increase in interleukin 5 receptor expression beyond tissue eosinophils in inflammatory bowel disease |
| topic | eosinophil interleukin-5 receptor inflammatory bowel disease pathogenesis proinflammatory cytokine |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1589421/full |
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