GHRH-stimulated pituitary small extracellular vesicles inhibit hepatocyte proliferation and IGF-1 expression by its cargo miR-375-3p
Abstract Small extracellular vesicles (sEV) have emerged as a novel mode of intercellular material transport and information transmission. It has been suggested hormones may regulate the production and function of sEV. However, the specific impact of growth hormone-releasing hormone (GHRH) on pituit...
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| Format: | Article |
| Language: | English |
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BMC
2024-10-01
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| Series: | Journal of Nanobiotechnology |
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| Online Access: | https://doi.org/10.1186/s12951-024-02857-y |
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| author | Jiali Xiong Yuxuan Wang Hailong Wang Junyi Luo Ting Chen Jiajie Sun Qianyun Xi Yongliang Zhang |
| author_facet | Jiali Xiong Yuxuan Wang Hailong Wang Junyi Luo Ting Chen Jiajie Sun Qianyun Xi Yongliang Zhang |
| author_sort | Jiali Xiong |
| collection | DOAJ |
| description | Abstract Small extracellular vesicles (sEV) have emerged as a novel mode of intercellular material transport and information transmission. It has been suggested hormones may regulate the production and function of sEV. However, the specific impact of growth hormone-releasing hormone (GHRH) on pituitary sEV production and the role of sEV in the regulation of the GHRH-GH-IGF axis has not been previously reported. The results of the present study demonstrated that GHRH increased the production of pituitary sEV by promoting the expression of Rab27a. More importantly, GHRH induced alterations in protein and miRNA levels within GH3-sEV components. Notably, GH3-sEV with GHRH treatment exhibited the enhanced ability to impede BRL 3A cell proliferation and the expression of IGF-1. Conclusively, for the first time, we corroborate the influence of GHRH on pituitary sEV, thereby presenting novel evidence for how sEV participates in the balance of the GHRH-GH-IGF axis. Importantly, this study provides new insight into a novel balance mechanism mediated by sEV within the endocrine system. Graphical Abstract |
| format | Article |
| id | doaj-art-e46aed6d2b9e40da813e74d51441f3c7 |
| institution | OA Journals |
| issn | 1477-3155 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj-art-e46aed6d2b9e40da813e74d51441f3c72025-08-20T02:11:54ZengBMCJournal of Nanobiotechnology1477-31552024-10-0122111510.1186/s12951-024-02857-yGHRH-stimulated pituitary small extracellular vesicles inhibit hepatocyte proliferation and IGF-1 expression by its cargo miR-375-3pJiali Xiong0Yuxuan Wang1Hailong Wang2Junyi Luo3Ting Chen4Jiajie Sun5Qianyun Xi6Yongliang Zhang7College of Medicine, Jiaxing UniversityCollege of Animal Science, Guangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Swine and Poultry Breeding Industry, South China Agricultural UniversityCollege of Animal Science, Guangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Swine and Poultry Breeding Industry, South China Agricultural UniversityCollege of Animal Science, Guangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Swine and Poultry Breeding Industry, South China Agricultural UniversityCollege of Animal Science, Guangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Swine and Poultry Breeding Industry, South China Agricultural UniversityCollege of Animal Science, Guangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Swine and Poultry Breeding Industry, South China Agricultural UniversityCollege of Animal Science, Guangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Swine and Poultry Breeding Industry, South China Agricultural UniversityCollege of Animal Science, Guangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Swine and Poultry Breeding Industry, South China Agricultural UniversityAbstract Small extracellular vesicles (sEV) have emerged as a novel mode of intercellular material transport and information transmission. It has been suggested hormones may regulate the production and function of sEV. However, the specific impact of growth hormone-releasing hormone (GHRH) on pituitary sEV production and the role of sEV in the regulation of the GHRH-GH-IGF axis has not been previously reported. The results of the present study demonstrated that GHRH increased the production of pituitary sEV by promoting the expression of Rab27a. More importantly, GHRH induced alterations in protein and miRNA levels within GH3-sEV components. Notably, GH3-sEV with GHRH treatment exhibited the enhanced ability to impede BRL 3A cell proliferation and the expression of IGF-1. Conclusively, for the first time, we corroborate the influence of GHRH on pituitary sEV, thereby presenting novel evidence for how sEV participates in the balance of the GHRH-GH-IGF axis. Importantly, this study provides new insight into a novel balance mechanism mediated by sEV within the endocrine system. Graphical Abstracthttps://doi.org/10.1186/s12951-024-02857-yGHRHPituitary small extracellular vesiclesHepatocytemiR-375-3pIGF-1 |
| spellingShingle | Jiali Xiong Yuxuan Wang Hailong Wang Junyi Luo Ting Chen Jiajie Sun Qianyun Xi Yongliang Zhang GHRH-stimulated pituitary small extracellular vesicles inhibit hepatocyte proliferation and IGF-1 expression by its cargo miR-375-3p Journal of Nanobiotechnology GHRH Pituitary small extracellular vesicles Hepatocyte miR-375-3p IGF-1 |
| title | GHRH-stimulated pituitary small extracellular vesicles inhibit hepatocyte proliferation and IGF-1 expression by its cargo miR-375-3p |
| title_full | GHRH-stimulated pituitary small extracellular vesicles inhibit hepatocyte proliferation and IGF-1 expression by its cargo miR-375-3p |
| title_fullStr | GHRH-stimulated pituitary small extracellular vesicles inhibit hepatocyte proliferation and IGF-1 expression by its cargo miR-375-3p |
| title_full_unstemmed | GHRH-stimulated pituitary small extracellular vesicles inhibit hepatocyte proliferation and IGF-1 expression by its cargo miR-375-3p |
| title_short | GHRH-stimulated pituitary small extracellular vesicles inhibit hepatocyte proliferation and IGF-1 expression by its cargo miR-375-3p |
| title_sort | ghrh stimulated pituitary small extracellular vesicles inhibit hepatocyte proliferation and igf 1 expression by its cargo mir 375 3p |
| topic | GHRH Pituitary small extracellular vesicles Hepatocyte miR-375-3p IGF-1 |
| url | https://doi.org/10.1186/s12951-024-02857-y |
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