Behavioral and physiological insights into cross-stage interactions in the desert locust

Abstract Gregarious desert locusts produce stage-specific pheromones that facilitate cohesive behavior in juveniles and synchronize maturation and mating in sexually mature adults. During locust outbreaks, merging populations result in cross-stage interactions, yet their impact on locust biology rem...

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Main Authors: Benjamin Fürstenau, Vincent O. Nyasembe, Hosea O. Mokaya, Hillary K. Kirwa, Angel Guerrero, Baldwyn Torto
Format: Article
Language:English
Published: Nature Portfolio 2025-06-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-08853-y
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author Benjamin Fürstenau
Vincent O. Nyasembe
Hosea O. Mokaya
Hillary K. Kirwa
Angel Guerrero
Baldwyn Torto
author_facet Benjamin Fürstenau
Vincent O. Nyasembe
Hosea O. Mokaya
Hillary K. Kirwa
Angel Guerrero
Baldwyn Torto
author_sort Benjamin Fürstenau
collection DOAJ
description Abstract Gregarious desert locusts produce stage-specific pheromones that facilitate cohesive behavior in juveniles and synchronize maturation and mating in sexually mature adults. During locust outbreaks, merging populations result in cross-stage interactions, yet their impact on locust biology remains poorly understood. This study tested the hypothesis that cross-stage interactions influence juvenile cohesion and physiological traits. Using behavioral assays and gas chromatography-mass spectrometry, we examined short- and long-term interactions between juvenile and adult desert locusts. In short-term (24 h) cage assays, the presence of adults did not significantly affect grouping behavior in gregarious 3rd instar nymphs, as measured by the mean distance between individuals. Likewise, overall, juvenile pheromone emissions, based on previously identified nymphal components, showed no significant differences regardless of adult presence. Cross-stage interactions also had no measurable effect on the development time of 3rd instar nymphs. In contrast, long-term assays showed that 1st instar nymphs grouped with adults matured faster and grew heavier than older nymphal instars and fledglings, and, as mature males, released higher levels of phenylacetonitrile (PAN). Additionally, adult females emerging from these interactions oviposited earlier and laid more eggs than those not exposed to adults as juveniles. These findings indicate that cross-stage interactions impact development uniquely across different gregarious locust stages. Additionally, they offer important insights into desert locust behavior and chemical ecology, which could aid in developing more effective management strategies.
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spelling doaj-art-e46aafef72d042f5830361e61476ab932025-08-20T03:27:09ZengNature PortfolioScientific Reports2045-23222025-06-0115111110.1038/s41598-025-08853-yBehavioral and physiological insights into cross-stage interactions in the desert locustBenjamin Fürstenau0Vincent O. Nyasembe1Hosea O. Mokaya2Hillary K. Kirwa3Angel Guerrero4Baldwyn Torto5Julius Kühn Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Ecological Chemistry, Plant Analysis and Stored Product ProtectionInternational Centre of Insect Physiology and Ecology (ICIPE), Behavioural & Chemical Ecology DepartmentInternational Centre of Insect Physiology and Ecology (ICIPE), Behavioural & Chemical Ecology DepartmentInternational Centre of Insect Physiology and Ecology (ICIPE), Behavioural & Chemical Ecology DepartmentDepartment of Biological Chemistry, Institute for Advanced Chemistry of Catalonia (IQAC-CSIC)International Centre of Insect Physiology and Ecology (ICIPE), Behavioural & Chemical Ecology DepartmentAbstract Gregarious desert locusts produce stage-specific pheromones that facilitate cohesive behavior in juveniles and synchronize maturation and mating in sexually mature adults. During locust outbreaks, merging populations result in cross-stage interactions, yet their impact on locust biology remains poorly understood. This study tested the hypothesis that cross-stage interactions influence juvenile cohesion and physiological traits. Using behavioral assays and gas chromatography-mass spectrometry, we examined short- and long-term interactions between juvenile and adult desert locusts. In short-term (24 h) cage assays, the presence of adults did not significantly affect grouping behavior in gregarious 3rd instar nymphs, as measured by the mean distance between individuals. Likewise, overall, juvenile pheromone emissions, based on previously identified nymphal components, showed no significant differences regardless of adult presence. Cross-stage interactions also had no measurable effect on the development time of 3rd instar nymphs. In contrast, long-term assays showed that 1st instar nymphs grouped with adults matured faster and grew heavier than older nymphal instars and fledglings, and, as mature males, released higher levels of phenylacetonitrile (PAN). Additionally, adult females emerging from these interactions oviposited earlier and laid more eggs than those not exposed to adults as juveniles. These findings indicate that cross-stage interactions impact development uniquely across different gregarious locust stages. Additionally, they offer important insights into desert locust behavior and chemical ecology, which could aid in developing more effective management strategies.https://doi.org/10.1038/s41598-025-08853-ySchistocerca gregariaSynchronous maturationPheromonePhenylacetonitrile
spellingShingle Benjamin Fürstenau
Vincent O. Nyasembe
Hosea O. Mokaya
Hillary K. Kirwa
Angel Guerrero
Baldwyn Torto
Behavioral and physiological insights into cross-stage interactions in the desert locust
Scientific Reports
Schistocerca gregaria
Synchronous maturation
Pheromone
Phenylacetonitrile
title Behavioral and physiological insights into cross-stage interactions in the desert locust
title_full Behavioral and physiological insights into cross-stage interactions in the desert locust
title_fullStr Behavioral and physiological insights into cross-stage interactions in the desert locust
title_full_unstemmed Behavioral and physiological insights into cross-stage interactions in the desert locust
title_short Behavioral and physiological insights into cross-stage interactions in the desert locust
title_sort behavioral and physiological insights into cross stage interactions in the desert locust
topic Schistocerca gregaria
Synchronous maturation
Pheromone
Phenylacetonitrile
url https://doi.org/10.1038/s41598-025-08853-y
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