Gellable silk fibroin-polyethylene sponge for hemostasis
Traditional haemostatic materials generally have slow hemostasis rate and poor biocompatibility. This paper reports on the haemostatic properties and mechanism of silk fibroin (SF). SF-PEG sponge that could be solubilised and changed to gel form by blood was fabricated through mixing SF and polyethy...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2019.1699805 |
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| author | Wei Wei Jian Liu ZhiBin Peng Min Liang YanSong Wang XiaoQin Wang |
| author_facet | Wei Wei Jian Liu ZhiBin Peng Min Liang YanSong Wang XiaoQin Wang |
| author_sort | Wei Wei |
| collection | DOAJ |
| description | Traditional haemostatic materials generally have slow hemostasis rate and poor biocompatibility. This paper reports on the haemostatic properties and mechanism of silk fibroin (SF). SF-PEG sponge that could be solubilised and changed to gel form by blood was fabricated through mixing SF and polyethylene glycol (PEG, 1500 Da) followed by lyophilisation of the mixed solution. SF-PEG sponge, together with control samples of SF sponge (no PEG) and a commercially available haemostatic material, gelatine sponge, were subjected to the hemostasis tests using a liver trauma model of rabbit. The results showed that SF was superior to gelatine sponge in hemostasis time (136.17 ± 62.27 s and 249.83 ± 29.18 s) and blood loss (2.16 ± 1.27 g vs. 4.97 ± 1.44 g). Furthermore, in vitro experiments indicated SF-PEG sol-gel transition promoted platelet adhesion and aggregation, as well as platelet-fibrinogen interaction. Therefore, except for the physical blocking of bleeding port due to PEG-induced SF fast gelation, SF might also have an impact on blood coagulation process, a phenomenon that has not been reported before. In conclusion, SF is a new type of haemostatic material that might be able to meet the requirements of speed, efficiency and biosafety in a variety of clinical applications. |
| format | Article |
| id | doaj-art-e467e233c13944d8878851716dbfe5a3 |
| institution | Kabale University |
| issn | 2169-1401 2169-141X |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj-art-e467e233c13944d8878851716dbfe5a32025-08-20T03:41:51ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2020-01-01481283610.1080/21691401.2019.1699805Gellable silk fibroin-polyethylene sponge for hemostasisWei Wei0Jian Liu1ZhiBin Peng2Min Liang3YanSong Wang4XiaoQin Wang5Department of Orthopaedics, The 1st Affiliated Hospital of Harbin Medical University, Harbin, ChinaNational Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, ChinaDepartment of Orthopaedics, The 1st Affiliated Hospital of Harbin Medical University, Harbin, ChinaDepartment of Orthopaedics, The 1st Affiliated Hospital of Harbin Medical University, Harbin, ChinaDepartment of Orthopaedics, The 1st Affiliated Hospital of Harbin Medical University, Harbin, ChinaNational Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, ChinaTraditional haemostatic materials generally have slow hemostasis rate and poor biocompatibility. This paper reports on the haemostatic properties and mechanism of silk fibroin (SF). SF-PEG sponge that could be solubilised and changed to gel form by blood was fabricated through mixing SF and polyethylene glycol (PEG, 1500 Da) followed by lyophilisation of the mixed solution. SF-PEG sponge, together with control samples of SF sponge (no PEG) and a commercially available haemostatic material, gelatine sponge, were subjected to the hemostasis tests using a liver trauma model of rabbit. The results showed that SF was superior to gelatine sponge in hemostasis time (136.17 ± 62.27 s and 249.83 ± 29.18 s) and blood loss (2.16 ± 1.27 g vs. 4.97 ± 1.44 g). Furthermore, in vitro experiments indicated SF-PEG sol-gel transition promoted platelet adhesion and aggregation, as well as platelet-fibrinogen interaction. Therefore, except for the physical blocking of bleeding port due to PEG-induced SF fast gelation, SF might also have an impact on blood coagulation process, a phenomenon that has not been reported before. In conclusion, SF is a new type of haemostatic material that might be able to meet the requirements of speed, efficiency and biosafety in a variety of clinical applications.https://www.tandfonline.com/doi/10.1080/21691401.2019.1699805Silk fibroinphysiological blood coagulationphysical adsorptionnew haemostatic material |
| spellingShingle | Wei Wei Jian Liu ZhiBin Peng Min Liang YanSong Wang XiaoQin Wang Gellable silk fibroin-polyethylene sponge for hemostasis Artificial Cells, Nanomedicine, and Biotechnology Silk fibroin physiological blood coagulation physical adsorption new haemostatic material |
| title | Gellable silk fibroin-polyethylene sponge for hemostasis |
| title_full | Gellable silk fibroin-polyethylene sponge for hemostasis |
| title_fullStr | Gellable silk fibroin-polyethylene sponge for hemostasis |
| title_full_unstemmed | Gellable silk fibroin-polyethylene sponge for hemostasis |
| title_short | Gellable silk fibroin-polyethylene sponge for hemostasis |
| title_sort | gellable silk fibroin polyethylene sponge for hemostasis |
| topic | Silk fibroin physiological blood coagulation physical adsorption new haemostatic material |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2019.1699805 |
| work_keys_str_mv | AT weiwei gellablesilkfibroinpolyethylenespongeforhemostasis AT jianliu gellablesilkfibroinpolyethylenespongeforhemostasis AT zhibinpeng gellablesilkfibroinpolyethylenespongeforhemostasis AT minliang gellablesilkfibroinpolyethylenespongeforhemostasis AT yansongwang gellablesilkfibroinpolyethylenespongeforhemostasis AT xiaoqinwang gellablesilkfibroinpolyethylenespongeforhemostasis |