Diurnal and daily fluctuations in levels of the urinary oxidative stress marker 8-hydroxyguanosine in spot urine samples
Abstract Background Urinary 8-hydroxyguanosine (8-OHGuo) levels serve as a biomarker for oxidative stress and hydroxyl radical–induced RNA damage. Evaluating the diurnal and daily fluctuations in urinary 8-OHGuo excretion levels is essential for understanding its implications. However, research in t...
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2025-01-01
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| Series: | Genes and Environment |
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| Online Access: | https://doi.org/10.1186/s41021-025-00324-0 |
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| author | Yun-Shan Li Koichi Fujisawa Kazuaki Kawai |
| author_facet | Yun-Shan Li Koichi Fujisawa Kazuaki Kawai |
| author_sort | Yun-Shan Li |
| collection | DOAJ |
| description | Abstract Background Urinary 8-hydroxyguanosine (8-OHGuo) levels serve as a biomarker for oxidative stress and hydroxyl radical–induced RNA damage. Evaluating the diurnal and daily fluctuations in urinary 8-OHGuo excretion levels is essential for understanding its implications. However, research in this area remains limited. In this study, we aim to investigate the diurnal and daily fluctuations in 8-OHGuo levels as well as the factors that influence these variations, using spot urine samples. Methods Urine samples were collected from seven healthy participants during each urination from the time of awakening until 24:00 h to evaluate diurnal variations. To assess daily fluctuations, urine samples were collected from 18 healthy participants at the time of awakening for 23 consecutive days. The urinary 8-OHGuo levels were measured using an HPLC-ECD method. Results No significant variations were observed in the diurnal levels of urinary 8-OHGuo among non-smokers. Conversely, the daily variation of 8-OHGuo in the urine of the smoker was significant, with a coefficient of variation of 18.71%. Each individual maintained a characteristic value despite some diurnal fluctuations. Furthermore, the daily levels of 8-OHGuo exhibited a range of variations influenced by lifestyle factors, including mental state, sleep duration, smoking, menstrual cycle, and dietary habits. Conclusion As a specific marker of RNA oxidation, 8-OHGuo provides unique insights distinct from those provided by the widely used DNA oxidation marker 8-hydroxydeoxyguanosine as an indicator of oxidative stress. Urinary 8-OHGuo could serve as a valuable biomarker for managing and preventing oxidative stress–related diseases, provided that the specific range of daily variations is established. The high daily variation in urinary 8-OHGuo levels necessitates the use of multiple samples to accurately determine individual levels. However, further research with large sample sizes will help to validate these findings. |
| format | Article |
| id | doaj-art-e4676fb4f6724eeda769c241a4c77865 |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
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| series | Genes and Environment |
| spelling | doaj-art-e4676fb4f6724eeda769c241a4c778652025-01-26T12:52:45ZengBMCGenes and Environment1880-70622025-01-014711810.1186/s41021-025-00324-0Diurnal and daily fluctuations in levels of the urinary oxidative stress marker 8-hydroxyguanosine in spot urine samplesYun-Shan Li0Koichi Fujisawa1Kazuaki Kawai2Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, JapanDepartment of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, JapanDepartment of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, JapanAbstract Background Urinary 8-hydroxyguanosine (8-OHGuo) levels serve as a biomarker for oxidative stress and hydroxyl radical–induced RNA damage. Evaluating the diurnal and daily fluctuations in urinary 8-OHGuo excretion levels is essential for understanding its implications. However, research in this area remains limited. In this study, we aim to investigate the diurnal and daily fluctuations in 8-OHGuo levels as well as the factors that influence these variations, using spot urine samples. Methods Urine samples were collected from seven healthy participants during each urination from the time of awakening until 24:00 h to evaluate diurnal variations. To assess daily fluctuations, urine samples were collected from 18 healthy participants at the time of awakening for 23 consecutive days. The urinary 8-OHGuo levels were measured using an HPLC-ECD method. Results No significant variations were observed in the diurnal levels of urinary 8-OHGuo among non-smokers. Conversely, the daily variation of 8-OHGuo in the urine of the smoker was significant, with a coefficient of variation of 18.71%. Each individual maintained a characteristic value despite some diurnal fluctuations. Furthermore, the daily levels of 8-OHGuo exhibited a range of variations influenced by lifestyle factors, including mental state, sleep duration, smoking, menstrual cycle, and dietary habits. Conclusion As a specific marker of RNA oxidation, 8-OHGuo provides unique insights distinct from those provided by the widely used DNA oxidation marker 8-hydroxydeoxyguanosine as an indicator of oxidative stress. Urinary 8-OHGuo could serve as a valuable biomarker for managing and preventing oxidative stress–related diseases, provided that the specific range of daily variations is established. The high daily variation in urinary 8-OHGuo levels necessitates the use of multiple samples to accurately determine individual levels. However, further research with large sample sizes will help to validate these findings.https://doi.org/10.1186/s41021-025-00324-0Daily fluctuationDiurnal variationOxidative stressUrine8-Hydroxyguanosine |
| spellingShingle | Yun-Shan Li Koichi Fujisawa Kazuaki Kawai Diurnal and daily fluctuations in levels of the urinary oxidative stress marker 8-hydroxyguanosine in spot urine samples Genes and Environment Daily fluctuation Diurnal variation Oxidative stress Urine 8-Hydroxyguanosine |
| title | Diurnal and daily fluctuations in levels of the urinary oxidative stress marker 8-hydroxyguanosine in spot urine samples |
| title_full | Diurnal and daily fluctuations in levels of the urinary oxidative stress marker 8-hydroxyguanosine in spot urine samples |
| title_fullStr | Diurnal and daily fluctuations in levels of the urinary oxidative stress marker 8-hydroxyguanosine in spot urine samples |
| title_full_unstemmed | Diurnal and daily fluctuations in levels of the urinary oxidative stress marker 8-hydroxyguanosine in spot urine samples |
| title_short | Diurnal and daily fluctuations in levels of the urinary oxidative stress marker 8-hydroxyguanosine in spot urine samples |
| title_sort | diurnal and daily fluctuations in levels of the urinary oxidative stress marker 8 hydroxyguanosine in spot urine samples |
| topic | Daily fluctuation Diurnal variation Oxidative stress Urine 8-Hydroxyguanosine |
| url | https://doi.org/10.1186/s41021-025-00324-0 |
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