Research on the Diffusion of Vs55 Permeability in Rat Kidneys Based on Computer Tomography
Cryopreservation is the usual method for long-term preservation of natural and engineered tissues. However, homogeneous loading of tissues with CPAs constitutes one of the major hurdles in tissue cryopreservation. In this study, computer tomography (CT) was used as a non-invasive imaging method to d...
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| Format: | Article |
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Journal of Refrigeration Magazines Agency Co., Ltd.
2020-01-01
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| Series: | Zhileng xuebao |
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| Online Access: | http://www.zhilengxuebao.com/thesisDetails#10.3969/j.issn.0253-4339.2020.03.160 |
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| author | Liu Ke Xu Yi Feng Haikao Dang Hangyu Yang Guoliang Li Xin |
| author_facet | Liu Ke Xu Yi Feng Haikao Dang Hangyu Yang Guoliang Li Xin |
| author_sort | Liu Ke |
| collection | DOAJ |
| description | Cryopreservation is the usual method for long-term preservation of natural and engineered tissues. However, homogeneous loading of tissues with CPAs constitutes one of the major hurdles in tissue cryopreservation. In this study, computer tomography (CT) was used as a non-invasive imaging method to determine the effective diffusion of Vs55 in tissue-engineered collagen scaffolds, and the effective diffusion constant (Deff) was calculated by the theoretic model. The kidney activation energy was calculated according to the effective diffusion constant obtained by CT. The results demonstrated that the higher the loading concentration, the faster the rate of diffusion. When Vs55 concentration was 8.4 mol/L, the diffusion rates were 1.77××10-6cm2/s at 4 °C,2.94××10-6cm2/s at 22 °C, and 3.93××10-6cm2/s at 37 °C, respectively. The model was demonstrated to predict the spatial and temporal distribution of Vs55 in the kidney. The coefficient of determination (R2) and the mean relative error (MRE) were 0.960–0.993 and 3.07%–6.74%, respectively. This observation was temperature-dependent, with slower permeation at lower temperatures. This study demonstrated the effectiveness of CT to measure Vs55 permeation into rat kidney. |
| format | Article |
| id | doaj-art-e45f6c847231436ca8cdc73b7813260e |
| institution | OA Journals |
| issn | 0253-4339 |
| language | zho |
| publishDate | 2020-01-01 |
| publisher | Journal of Refrigeration Magazines Agency Co., Ltd. |
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| series | Zhileng xuebao |
| spelling | doaj-art-e45f6c847231436ca8cdc73b7813260e2025-08-20T02:03:01ZzhoJournal of Refrigeration Magazines Agency Co., Ltd.Zhileng xuebao0253-43392020-01-014166508452Research on the Diffusion of Vs55 Permeability in Rat Kidneys Based on Computer TomographyLiu KeXu YiFeng HaikaoDang HangyuYang GuoliangLi XinCryopreservation is the usual method for long-term preservation of natural and engineered tissues. However, homogeneous loading of tissues with CPAs constitutes one of the major hurdles in tissue cryopreservation. In this study, computer tomography (CT) was used as a non-invasive imaging method to determine the effective diffusion of Vs55 in tissue-engineered collagen scaffolds, and the effective diffusion constant (Deff) was calculated by the theoretic model. The kidney activation energy was calculated according to the effective diffusion constant obtained by CT. The results demonstrated that the higher the loading concentration, the faster the rate of diffusion. When Vs55 concentration was 8.4 mol/L, the diffusion rates were 1.77××10-6cm2/s at 4 °C,2.94××10-6cm2/s at 22 °C, and 3.93××10-6cm2/s at 37 °C, respectively. The model was demonstrated to predict the spatial and temporal distribution of Vs55 in the kidney. The coefficient of determination (R2) and the mean relative error (MRE) were 0.960–0.993 and 3.07%–6.74%, respectively. This observation was temperature-dependent, with slower permeation at lower temperatures. This study demonstrated the effectiveness of CT to measure Vs55 permeation into rat kidney.http://www.zhilengxuebao.com/thesisDetails#10.3969/j.issn.0253-4339.2020.03.160CTvisualizationVs55effective diffusion coefficient |
| spellingShingle | Liu Ke Xu Yi Feng Haikao Dang Hangyu Yang Guoliang Li Xin Research on the Diffusion of Vs55 Permeability in Rat Kidneys Based on Computer Tomography Zhileng xuebao CT visualization Vs55 effective diffusion coefficient |
| title | Research on the Diffusion of Vs55 Permeability in Rat Kidneys Based on Computer Tomography |
| title_full | Research on the Diffusion of Vs55 Permeability in Rat Kidneys Based on Computer Tomography |
| title_fullStr | Research on the Diffusion of Vs55 Permeability in Rat Kidneys Based on Computer Tomography |
| title_full_unstemmed | Research on the Diffusion of Vs55 Permeability in Rat Kidneys Based on Computer Tomography |
| title_short | Research on the Diffusion of Vs55 Permeability in Rat Kidneys Based on Computer Tomography |
| title_sort | research on the diffusion of vs55 permeability in rat kidneys based on computer tomography |
| topic | CT visualization Vs55 effective diffusion coefficient |
| url | http://www.zhilengxuebao.com/thesisDetails#10.3969/j.issn.0253-4339.2020.03.160 |
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