Osteoclast-like activity of U937 cells, peripheral blood mononuclear cells, and periodontal ligament fibroblasts subjected to mechanical stress by centrifugal force
Several in vitro models have investigated the consequences of mechanical stimuli on osteoclasts (OCs). However, the mechanisms whereby mechanical forces trigger osteoclast responses remain poorly understood, and the generation of reactive oxygen species (ROS) and their relationship with bone reso...
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Pontificia Universidad Javeriana
2022-11-01
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| Series: | Universitas Scientiarum |
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| Online Access: | https://revistas.javeriana.edu.co/index.php/scientarium/article/view/35609 |
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| author | Carlos Guerrero Fonseca Pedro Moreno Beltrán Angie Bedoya Rodríguez Bibiana Pazos Valencia Juan Sebastián Bedoya Trujillo Orlando Acosta |
| author_facet | Carlos Guerrero Fonseca Pedro Moreno Beltrán Angie Bedoya Rodríguez Bibiana Pazos Valencia Juan Sebastián Bedoya Trujillo Orlando Acosta |
| author_sort | Carlos Guerrero Fonseca |
| collection | DOAJ |
| description | Several in vitro models have investigated the consequences of mechanical stimuli on osteoclasts (OCs).
However, the mechanisms whereby mechanical forces trigger osteoclast responses remain poorly
understood, and the generation of reactive oxygen species (ROS) and their relationship with bone
resorption in OCs under the influence of mechanical forces require investigation. The present work
examined the role of centrifugal force application on ROS production and its effect on osteoclast activity
and differentiation. Human U937 macrophage cells, peripheral blood mononuclear cells (PBMCs),
and periodontal ligament (PDL) fibroblasts treated with polyethylene glycol (PEG) or N-acetylcysteine
(NAC) were subject to centrifugal force. Osteoclast markers such as tartrate-resistant acid phosphatase
(TRAP) and bone resorption activities were measured. ROS levels and actin ring formation were
determined. Also, U937 cell responses to centrifugal force and PEG-induced fusion were studied.
Individual cells subjected to centrifugal exertion increased their ROS levels, formed actin-like rings,
revealed TRAP expression and bone resorption activities, and expressed typical osteoclast markers.
Control PEG-fused U937 cells also showed these effects, and cell treatment with NAC stopped all
these responses. Centrifugal force, as well as PEG-induced cell fusion, can promote osteoclast-like
features, including oxidative stress. The present experimental model allowed us to understand the
mechanisms underlying the osteoclast differentiation associated with ROS production stimulated by
mechanical compressive force, where NAC can contribute to reducing this oxidative stress condition. |
| format | Article |
| id | doaj-art-e45cd9fe7f24413e8c09a4267245100e |
| institution | OA Journals |
| issn | 0122-7483 2027-1352 |
| language | English |
| publishDate | 2022-11-01 |
| publisher | Pontificia Universidad Javeriana |
| record_format | Article |
| series | Universitas Scientiarum |
| spelling | doaj-art-e45cd9fe7f24413e8c09a4267245100e2025-08-20T02:21:34ZengPontificia Universidad JaverianaUniversitas Scientiarum0122-74832027-13522022-11-0127333133510.11144/Javeriana.SC273.oaouOsteoclast-like activity of U937 cells, peripheral blood mononuclear cells, and periodontal ligament fibroblasts subjected to mechanical stress by centrifugal forceCarlos Guerrero Fonseca0https://orcid.org/0000-0003-2863-6345Pedro Moreno Beltrán1https://orcid.org/0000-0002-8477-9220Angie Bedoya Rodríguez2https://orcid.org/0000-0003-2293-6128Bibiana Pazos Valencia3https://orcid.org/0000-0002-8659-9047Juan Sebastián Bedoya Trujillo4https://orcid.org/0000-0002-4727-1946Orlando Acosta5https://orcid.org/0000-0002-1267-098XDepartment of Physiological Sciences, Faculty of Medicine, Universidad Nacional de ColombiaDepartment of Physiological Sciences, Faculty of Medicine, Universidad Nacional de ColombiaDepartment of Physiological Sciences, Faculty of Medicine, Universidad Nacional de ColombiaDepartment of Orthodontics, Faculty of Dentistry, Universidad Nacional de ColombiaDepartment of Orthodontics, Faculty of Dentistry, Universidad Nacional de ColombiaDepartment of Physiological Sciences, Faculty of Medicine, Universidad Nacional de ColombiaSeveral in vitro models have investigated the consequences of mechanical stimuli on osteoclasts (OCs). However, the mechanisms whereby mechanical forces trigger osteoclast responses remain poorly understood, and the generation of reactive oxygen species (ROS) and their relationship with bone resorption in OCs under the influence of mechanical forces require investigation. The present work examined the role of centrifugal force application on ROS production and its effect on osteoclast activity and differentiation. Human U937 macrophage cells, peripheral blood mononuclear cells (PBMCs), and periodontal ligament (PDL) fibroblasts treated with polyethylene glycol (PEG) or N-acetylcysteine (NAC) were subject to centrifugal force. Osteoclast markers such as tartrate-resistant acid phosphatase (TRAP) and bone resorption activities were measured. ROS levels and actin ring formation were determined. Also, U937 cell responses to centrifugal force and PEG-induced fusion were studied. Individual cells subjected to centrifugal exertion increased their ROS levels, formed actin-like rings, revealed TRAP expression and bone resorption activities, and expressed typical osteoclast markers. Control PEG-fused U937 cells also showed these effects, and cell treatment with NAC stopped all these responses. Centrifugal force, as well as PEG-induced cell fusion, can promote osteoclast-like features, including oxidative stress. The present experimental model allowed us to understand the mechanisms underlying the osteoclast differentiation associated with ROS production stimulated by mechanical compressive force, where NAC can contribute to reducing this oxidative stress condition.https://revistas.javeriana.edu.co/index.php/scientarium/article/view/35609bone resorption;centrifugal force;monocyte fusion;periodontal ligament;osteoclast activity;polyethylene glycol. |
| spellingShingle | Carlos Guerrero Fonseca Pedro Moreno Beltrán Angie Bedoya Rodríguez Bibiana Pazos Valencia Juan Sebastián Bedoya Trujillo Orlando Acosta Osteoclast-like activity of U937 cells, peripheral blood mononuclear cells, and periodontal ligament fibroblasts subjected to mechanical stress by centrifugal force Universitas Scientiarum bone resorption; centrifugal force; monocyte fusion; periodontal ligament; osteoclast activity; polyethylene glycol. |
| title | Osteoclast-like activity of U937 cells, peripheral blood mononuclear cells, and periodontal ligament fibroblasts subjected to mechanical stress by centrifugal force |
| title_full | Osteoclast-like activity of U937 cells, peripheral blood mononuclear cells, and periodontal ligament fibroblasts subjected to mechanical stress by centrifugal force |
| title_fullStr | Osteoclast-like activity of U937 cells, peripheral blood mononuclear cells, and periodontal ligament fibroblasts subjected to mechanical stress by centrifugal force |
| title_full_unstemmed | Osteoclast-like activity of U937 cells, peripheral blood mononuclear cells, and periodontal ligament fibroblasts subjected to mechanical stress by centrifugal force |
| title_short | Osteoclast-like activity of U937 cells, peripheral blood mononuclear cells, and periodontal ligament fibroblasts subjected to mechanical stress by centrifugal force |
| title_sort | osteoclast like activity of u937 cells peripheral blood mononuclear cells and periodontal ligament fibroblasts subjected to mechanical stress by centrifugal force |
| topic | bone resorption; centrifugal force; monocyte fusion; periodontal ligament; osteoclast activity; polyethylene glycol. |
| url | https://revistas.javeriana.edu.co/index.php/scientarium/article/view/35609 |
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