Susceptibility of swine cells and domestic pigs to SARS-CoV-2
The emergence of SARS-CoV-2 has resulted in an ongoing global pandemic with significant morbidity, mortality, and economic consequences. The susceptibility of different animal species to SARS-CoV-2 is of concern due to the potential for interspecies transmission, and the requirement for pre-clinical...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2020.1831405 |
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| author | David A. Meekins Igor Morozov Jessie D. Trujillo Natasha N. Gaudreault Dashzeveg Bold Mariano Carossino Bianca L. Artiaga Sabarish V. Indran Taeyong Kwon Velmurugan Balaraman Daniel W. Madden Heinz Feldmann Jamie Henningson Wenjun Ma Udeni B. R. Balasuriya Juergen A. Richt |
| author_facet | David A. Meekins Igor Morozov Jessie D. Trujillo Natasha N. Gaudreault Dashzeveg Bold Mariano Carossino Bianca L. Artiaga Sabarish V. Indran Taeyong Kwon Velmurugan Balaraman Daniel W. Madden Heinz Feldmann Jamie Henningson Wenjun Ma Udeni B. R. Balasuriya Juergen A. Richt |
| author_sort | David A. Meekins |
| collection | DOAJ |
| description | The emergence of SARS-CoV-2 has resulted in an ongoing global pandemic with significant morbidity, mortality, and economic consequences. The susceptibility of different animal species to SARS-CoV-2 is of concern due to the potential for interspecies transmission, and the requirement for pre-clinical animal models to develop effective countermeasures. In the current study, we determined the ability of SARS-CoV-2 to (i) replicate in porcine cell lines, (ii) establish infection in domestic pigs via experimental oral/intranasal/intratracheal inoculation, and (iii) transmit to co-housed naïve sentinel pigs. SARS-CoV-2 was able to replicate in two different porcine cell lines with cytopathic effects. Interestingly, none of the SARS-CoV-2-inoculated pigs showed evidence of clinical signs, viral replication or SARS-CoV-2-specific antibody responses. Moreover, none of the sentinel pigs displayed markers of SARS-CoV-2 infection. These data indicate that although different porcine cell lines are permissive to SARS-CoV-2, five-week old pigs are not susceptible to infection via oral/intranasal/intratracheal challenge. Pigs are therefore unlikely to be significant carriers of SARS-CoV-2 and are not a suitable pre-clinical animal model to study SARS-CoV-2 pathogenesis or efficacy of respective vaccines or therapeutics. |
| format | Article |
| id | doaj-art-e4589c3202cf495da942dfffa8ce8369 |
| institution | OA Journals |
| issn | 2222-1751 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-e4589c3202cf495da942dfffa8ce83692025-08-20T02:15:52ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512020-01-01912278228810.1080/22221751.2020.1831405Susceptibility of swine cells and domestic pigs to SARS-CoV-2David A. Meekins0Igor Morozov1Jessie D. Trujillo2Natasha N. Gaudreault3Dashzeveg Bold4Mariano Carossino5Bianca L. Artiaga6Sabarish V. Indran7Taeyong Kwon8Velmurugan Balaraman9Daniel W. Madden10Heinz Feldmann11Jamie Henningson12Wenjun Ma13Udeni B. R. Balasuriya14Juergen A. Richt15Center of Excellence for Emerging and Zoonotic Animal Diseases, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USACenter of Excellence for Emerging and Zoonotic Animal Diseases, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USACenter of Excellence for Emerging and Zoonotic Animal Diseases, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USACenter of Excellence for Emerging and Zoonotic Animal Diseases, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USACenter of Excellence for Emerging and Zoonotic Animal Diseases, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USALouisiana Animal Disease Diagnostic Laboratory and Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, USACenter of Excellence for Emerging and Zoonotic Animal Diseases, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USACenter of Excellence for Emerging and Zoonotic Animal Diseases, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USACenter of Excellence for Emerging and Zoonotic Animal Diseases, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USACenter of Excellence for Emerging and Zoonotic Animal Diseases, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USACenter of Excellence for Emerging and Zoonotic Animal Diseases, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USALaboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USACenter of Excellence for Emerging and Zoonotic Animal Diseases, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USACenter of Excellence for Emerging and Zoonotic Animal Diseases, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USALouisiana Animal Disease Diagnostic Laboratory and Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, USACenter of Excellence for Emerging and Zoonotic Animal Diseases, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USAThe emergence of SARS-CoV-2 has resulted in an ongoing global pandemic with significant morbidity, mortality, and economic consequences. The susceptibility of different animal species to SARS-CoV-2 is of concern due to the potential for interspecies transmission, and the requirement for pre-clinical animal models to develop effective countermeasures. In the current study, we determined the ability of SARS-CoV-2 to (i) replicate in porcine cell lines, (ii) establish infection in domestic pigs via experimental oral/intranasal/intratracheal inoculation, and (iii) transmit to co-housed naïve sentinel pigs. SARS-CoV-2 was able to replicate in two different porcine cell lines with cytopathic effects. Interestingly, none of the SARS-CoV-2-inoculated pigs showed evidence of clinical signs, viral replication or SARS-CoV-2-specific antibody responses. Moreover, none of the sentinel pigs displayed markers of SARS-CoV-2 infection. These data indicate that although different porcine cell lines are permissive to SARS-CoV-2, five-week old pigs are not susceptible to infection via oral/intranasal/intratracheal challenge. Pigs are therefore unlikely to be significant carriers of SARS-CoV-2 and are not a suitable pre-clinical animal model to study SARS-CoV-2 pathogenesis or efficacy of respective vaccines or therapeutics.https://www.tandfonline.com/doi/10.1080/22221751.2020.1831405SARS-CoV-2COVID-19swinecoronaviruspigsinfection models |
| spellingShingle | David A. Meekins Igor Morozov Jessie D. Trujillo Natasha N. Gaudreault Dashzeveg Bold Mariano Carossino Bianca L. Artiaga Sabarish V. Indran Taeyong Kwon Velmurugan Balaraman Daniel W. Madden Heinz Feldmann Jamie Henningson Wenjun Ma Udeni B. R. Balasuriya Juergen A. Richt Susceptibility of swine cells and domestic pigs to SARS-CoV-2 Emerging Microbes and Infections SARS-CoV-2 COVID-19 swine coronavirus pigs infection models |
| title | Susceptibility of swine cells and domestic pigs to SARS-CoV-2 |
| title_full | Susceptibility of swine cells and domestic pigs to SARS-CoV-2 |
| title_fullStr | Susceptibility of swine cells and domestic pigs to SARS-CoV-2 |
| title_full_unstemmed | Susceptibility of swine cells and domestic pigs to SARS-CoV-2 |
| title_short | Susceptibility of swine cells and domestic pigs to SARS-CoV-2 |
| title_sort | susceptibility of swine cells and domestic pigs to sars cov 2 |
| topic | SARS-CoV-2 COVID-19 swine coronavirus pigs infection models |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2020.1831405 |
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