In ovo sericin suppresses hepatic DNA demethylation in broilers at hatch
Over the years, the rearing period of the commercial broilers to attain the slaughter weight has reduced significantly. Hence, it emphasizes the importance of the period of embryonic development. It has been shown that inadequate nutritional supply to the embryo at the later phases can lead to vario...
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Elsevier
2025-05-01
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| Series: | Poultry Science |
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| author | Vaishali Gupta Amitav Bhattacharyya Yun-Ji Hwang Yang-Ho Choi |
| author_facet | Vaishali Gupta Amitav Bhattacharyya Yun-Ji Hwang Yang-Ho Choi |
| author_sort | Vaishali Gupta |
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| description | Over the years, the rearing period of the commercial broilers to attain the slaughter weight has reduced significantly. Hence, it emphasizes the importance of the period of embryonic development. It has been shown that inadequate nutritional supply to the embryo at the later phases can lead to various abnormalities. This adversely affects the hatchability and further the post-hatch performance of the chicks. This study attempted to study the effect of in ovo feeding of sericin on the developing Ross-308 embryos. Fertile eggs (n = 210) at 17.5 days of embryonic development (ED) were equally divided into five treatments based on the concentration of sericin fed. The treatments were: uninjected control (UCON), followed by different concentrations of sericin injected groups as 0SER (0 % sericin), 1.5SER (1.5 % sericin), 3.0SER (3.0 % sericin), and 4.5SER (4.5 % sericin). Hatch parameters across treatments did not differ significantly. Similarly, the organ (liver, yolk sac, gizzard, proventriculus and heart) indices and plasma antioxidant markers such as 2,2-Diphenyl-1-picrylhydrazyl – radical scavenging activity % (DPPH-RSA%) and malondialdehyde (MDA) content did not differ significantly across treatments. The hepatic mRNA expression of superoxide dismutase (SOD) was higher in 3.0SER treatment in comparison to 4.5SER. On the other hand, in ovo sericin downregulated the hepatic gene expression of DNA demethylation-related enzymes such as ten-eleven translocation methylcytosine dioxygenase 3 (TET3, p = 0.028) and methyl-CpG-binding domain protein 4 (MBD4, p = 0.007) compared to 0SER. Pearson's correlation analyses revealed a significant correlation between the hepatic gene expression of NADPH oxidase (NOX) related genes and DNA-demethylation-related genes (p < 0.01). Hence, in ovo sericin might not be potentially beneficial in improving the hatchability of broilers. Also, no notable effects on the antioxidant capacity of plasma was recorded. However, in ovo sericin downregulated the mRNA expression of some DNA demethylation-related genes which were significantly correlated with the expression of NOXs. Therefore, in ovo sericin feeding could suppress DNA demethylation which could in turn be beneficial to alleviate oxidative stress at hatch. |
| format | Article |
| id | doaj-art-e456038474264c9fbe41ece67e5d671f |
| institution | OA Journals |
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| publishDate | 2025-05-01 |
| publisher | Elsevier |
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| series | Poultry Science |
| spelling | doaj-art-e456038474264c9fbe41ece67e5d671f2025-08-20T01:51:41ZengElsevierPoultry Science0032-57912025-05-01104510507810.1016/j.psj.2025.105078In ovo sericin suppresses hepatic DNA demethylation in broilers at hatchVaishali Gupta0Amitav Bhattacharyya1Yun-Ji Hwang2Yang-Ho Choi3Department of Animal Science, Gyeongsang National University, Jinju 52828, Republic of Korea; Division of Applied Life Sciences (BK21 FOUR Program), Gyeongsang National University, Jinju 52828, Republic of KoreaDepartment of Animal Science, Gyeongsang National University, Jinju 52828, Republic of Korea; Department of Poultry Science, College of Veterinary Science and Animal Husbandry, DUVASU, Mathura 281001, IndiaDepartment of Animal Science, Gyeongsang National University, Jinju 52828, Republic of Korea; Division of Applied Life Sciences (BK21 FOUR Program), Gyeongsang National University, Jinju 52828, Republic of KoreaDepartment of Animal Science, Gyeongsang National University, Jinju 52828, Republic of Korea; Division of Applied Life Sciences (BK21 FOUR Program), Gyeongsang National University, Jinju 52828, Republic of Korea; Institute of Agriculture and Life Sciences, Gyeongsang National University, Jinju 52828, Republic of Korea; Corresponding author at: Department of Animal Science, Gyeongsang National University, Jinju 52828, Republic of Korea.Over the years, the rearing period of the commercial broilers to attain the slaughter weight has reduced significantly. Hence, it emphasizes the importance of the period of embryonic development. It has been shown that inadequate nutritional supply to the embryo at the later phases can lead to various abnormalities. This adversely affects the hatchability and further the post-hatch performance of the chicks. This study attempted to study the effect of in ovo feeding of sericin on the developing Ross-308 embryos. Fertile eggs (n = 210) at 17.5 days of embryonic development (ED) were equally divided into five treatments based on the concentration of sericin fed. The treatments were: uninjected control (UCON), followed by different concentrations of sericin injected groups as 0SER (0 % sericin), 1.5SER (1.5 % sericin), 3.0SER (3.0 % sericin), and 4.5SER (4.5 % sericin). Hatch parameters across treatments did not differ significantly. Similarly, the organ (liver, yolk sac, gizzard, proventriculus and heart) indices and plasma antioxidant markers such as 2,2-Diphenyl-1-picrylhydrazyl – radical scavenging activity % (DPPH-RSA%) and malondialdehyde (MDA) content did not differ significantly across treatments. The hepatic mRNA expression of superoxide dismutase (SOD) was higher in 3.0SER treatment in comparison to 4.5SER. On the other hand, in ovo sericin downregulated the hepatic gene expression of DNA demethylation-related enzymes such as ten-eleven translocation methylcytosine dioxygenase 3 (TET3, p = 0.028) and methyl-CpG-binding domain protein 4 (MBD4, p = 0.007) compared to 0SER. Pearson's correlation analyses revealed a significant correlation between the hepatic gene expression of NADPH oxidase (NOX) related genes and DNA-demethylation-related genes (p < 0.01). Hence, in ovo sericin might not be potentially beneficial in improving the hatchability of broilers. Also, no notable effects on the antioxidant capacity of plasma was recorded. However, in ovo sericin downregulated the mRNA expression of some DNA demethylation-related genes which were significantly correlated with the expression of NOXs. Therefore, in ovo sericin feeding could suppress DNA demethylation which could in turn be beneficial to alleviate oxidative stress at hatch.http://www.sciencedirect.com/science/article/pii/S0032579125003177BroilersIn ovo feedingSericinEpigeneticsDNA methylation |
| spellingShingle | Vaishali Gupta Amitav Bhattacharyya Yun-Ji Hwang Yang-Ho Choi In ovo sericin suppresses hepatic DNA demethylation in broilers at hatch Poultry Science Broilers In ovo feeding Sericin Epigenetics DNA methylation |
| title | In ovo sericin suppresses hepatic DNA demethylation in broilers at hatch |
| title_full | In ovo sericin suppresses hepatic DNA demethylation in broilers at hatch |
| title_fullStr | In ovo sericin suppresses hepatic DNA demethylation in broilers at hatch |
| title_full_unstemmed | In ovo sericin suppresses hepatic DNA demethylation in broilers at hatch |
| title_short | In ovo sericin suppresses hepatic DNA demethylation in broilers at hatch |
| title_sort | in ovo sericin suppresses hepatic dna demethylation in broilers at hatch |
| topic | Broilers In ovo feeding Sericin Epigenetics DNA methylation |
| url | http://www.sciencedirect.com/science/article/pii/S0032579125003177 |
| work_keys_str_mv | AT vaishaligupta inovosericinsuppresseshepaticdnademethylationinbroilersathatch AT amitavbhattacharyya inovosericinsuppresseshepaticdnademethylationinbroilersathatch AT yunjihwang inovosericinsuppresseshepaticdnademethylationinbroilersathatch AT yanghochoi inovosericinsuppresseshepaticdnademethylationinbroilersathatch |