Extract of Yellow Root (Arcangelisia Flava (L.) Merr.) from Several Regions in Kalimantan: Alkaloid Content and Cytotoxicity towards WiDr Colorectal Cancer Cells
Yellow root (Arcangelisia flava (L.) Merr.) has been scientifically known to have potential as an antimalarial, antibacterial, antioxidant, and anticancer. The purpose of this study was to determine the profile of alkaloid content and cytotoxicity of yellow root extract from several regions in Kalim...
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Indonesian Society for Cancer Chemoprevention
2020-06-01
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| Series: | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| Online Access: | https://ijcc.chemoprev.org/index.php/ijcc/article/view/293 |
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| author | Roihatul Mutiah Farenza Okta Kirana Rahmi Annisa Ana Rahmawati Ferry Sandra |
| author_facet | Roihatul Mutiah Farenza Okta Kirana Rahmi Annisa Ana Rahmawati Ferry Sandra |
| author_sort | Roihatul Mutiah |
| collection | DOAJ |
| description | Yellow root (Arcangelisia flava (L.) Merr.) has been scientifically known to have potential as an antimalarial, antibacterial, antioxidant, and anticancer. The purpose of this study was to determine the profile of alkaloid content and cytotoxicity of yellow root extract from several regions in Kalimantan. The alkaloid content was tested using the thin layer chromatography (TLC) method with dragendorf reagent. Cytotoxic in vitro test was conducted against WiDr colorectal cancer cells using the 3-(4,5-dimethylthiazol-2-il)-2,5-diphenyltetrazolium bromide (MTT) assay. Yellow roots were collected from Samarinda city, Banjarmasin city, Barito Timur regency, Malinau district, and Balikpapan City. The MTT inhibitory concentration 50 (IC50) of yellow root extracts were 573.308 μg/mL; 582.857 μg/mL; 296.326 μg/mL; 114.119 μg/mL; and 320.162 μg/mL respectively. Results of the compound identification indicated that alkaloid was found in A. flava from all regions. Alkaloids of A. flava extract should be investigated further in order to find possible active agent that could decrease the viability of WiDr colorectal cancer cells.
Keywords: Arcangelisia flava, Borneo, colorectal cancer, Kalimantan, WiDr cells. |
| format | Article |
| id | doaj-art-e451b337fb704e60b579ce0407ccfc9d |
| institution | Kabale University |
| issn | 2088-0197 2355-8989 |
| language | English |
| publishDate | 2020-06-01 |
| publisher | Indonesian Society for Cancer Chemoprevention |
| record_format | Article |
| series | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| spelling | doaj-art-e451b337fb704e60b579ce0407ccfc9d2025-08-20T03:52:11ZengIndonesian Society for Cancer ChemopreventionISCC (Indonesian Journal of Cancer Chemoprevention)2088-01972355-89892020-06-01112848910.14499/indonesianjcanchemoprev11iss2pp84-89206Extract of Yellow Root (Arcangelisia Flava (L.) Merr.) from Several Regions in Kalimantan: Alkaloid Content and Cytotoxicity towards WiDr Colorectal Cancer CellsRoihatul Mutiah0Farenza Okta Kirana1Rahmi Annisa2Ana Rahmawati3Ferry Sandra4Department of Pharmacy, Faculty of Medical and Health Sciences, Maulana Malik Ibrahim State Islamic University of MalangDepartment of Pharmacy, Faculty of Medical and Health Sciences, Maulana Malik Ibrahim State Islamic University of MalangDepartment of Pharmacy, Faculty of Medical and Health Sciences, Maulana Malik Ibrahim State Islamic University of MalangDepartment of Medical Education, Faculty of Medical and Health Sciences, Maulana Malik Ibrahim State Islamic University of MalangDepartment of Biochemistry and Molecular Biology, Division of Oral Biology, Faculty of Dentistry, Universitas TrisaktiYellow root (Arcangelisia flava (L.) Merr.) has been scientifically known to have potential as an antimalarial, antibacterial, antioxidant, and anticancer. The purpose of this study was to determine the profile of alkaloid content and cytotoxicity of yellow root extract from several regions in Kalimantan. The alkaloid content was tested using the thin layer chromatography (TLC) method with dragendorf reagent. Cytotoxic in vitro test was conducted against WiDr colorectal cancer cells using the 3-(4,5-dimethylthiazol-2-il)-2,5-diphenyltetrazolium bromide (MTT) assay. Yellow roots were collected from Samarinda city, Banjarmasin city, Barito Timur regency, Malinau district, and Balikpapan City. The MTT inhibitory concentration 50 (IC50) of yellow root extracts were 573.308 μg/mL; 582.857 μg/mL; 296.326 μg/mL; 114.119 μg/mL; and 320.162 μg/mL respectively. Results of the compound identification indicated that alkaloid was found in A. flava from all regions. Alkaloids of A. flava extract should be investigated further in order to find possible active agent that could decrease the viability of WiDr colorectal cancer cells. Keywords: Arcangelisia flava, Borneo, colorectal cancer, Kalimantan, WiDr cells.https://ijcc.chemoprev.org/index.php/ijcc/article/view/293 |
| spellingShingle | Roihatul Mutiah Farenza Okta Kirana Rahmi Annisa Ana Rahmawati Ferry Sandra Extract of Yellow Root (Arcangelisia Flava (L.) Merr.) from Several Regions in Kalimantan: Alkaloid Content and Cytotoxicity towards WiDr Colorectal Cancer Cells ISCC (Indonesian Journal of Cancer Chemoprevention) |
| title | Extract of Yellow Root (Arcangelisia Flava (L.) Merr.) from Several Regions in Kalimantan: Alkaloid Content and Cytotoxicity towards WiDr Colorectal Cancer Cells |
| title_full | Extract of Yellow Root (Arcangelisia Flava (L.) Merr.) from Several Regions in Kalimantan: Alkaloid Content and Cytotoxicity towards WiDr Colorectal Cancer Cells |
| title_fullStr | Extract of Yellow Root (Arcangelisia Flava (L.) Merr.) from Several Regions in Kalimantan: Alkaloid Content and Cytotoxicity towards WiDr Colorectal Cancer Cells |
| title_full_unstemmed | Extract of Yellow Root (Arcangelisia Flava (L.) Merr.) from Several Regions in Kalimantan: Alkaloid Content and Cytotoxicity towards WiDr Colorectal Cancer Cells |
| title_short | Extract of Yellow Root (Arcangelisia Flava (L.) Merr.) from Several Regions in Kalimantan: Alkaloid Content and Cytotoxicity towards WiDr Colorectal Cancer Cells |
| title_sort | extract of yellow root arcangelisia flava l merr from several regions in kalimantan alkaloid content and cytotoxicity towards widr colorectal cancer cells |
| url | https://ijcc.chemoprev.org/index.php/ijcc/article/view/293 |
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