Upper airways microbiota in antibiotic-naïve wheezing and healthy infants from the tropics of rural Ecuador.
<h4>Background</h4>Observations that the airway microbiome is disturbed in asthma may be confounded by the widespread use of antibiotics and inhaled steroids. We have therefore examined the oropharyngeal microbiome in early onset wheezing infants from a rural area of tropical Ecuador whe...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0046803&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850137360106258432 |
|---|---|
| author | Paul Andres Cardenas Philip J Cooper Michael J Cox Martha Chico Carlos Arias Miriam F Moffatt William Osmond Cookson |
| author_facet | Paul Andres Cardenas Philip J Cooper Michael J Cox Martha Chico Carlos Arias Miriam F Moffatt William Osmond Cookson |
| author_sort | Paul Andres Cardenas |
| collection | DOAJ |
| description | <h4>Background</h4>Observations that the airway microbiome is disturbed in asthma may be confounded by the widespread use of antibiotics and inhaled steroids. We have therefore examined the oropharyngeal microbiome in early onset wheezing infants from a rural area of tropical Ecuador where antibiotic usage is minimal and glucocorticoid usage is absent.<h4>Materials and methods</h4>We performed pyrosequencing of amplicons of the polymorphic bacterial 16S rRNA gene from oropharyngeal samples from 24 infants with non-infectious early onset wheezing and 24 healthy controls (average age 10.2 months). We analyzed microbial community structure and differences between cases and controls by QIIME software.<h4>Results</h4>We obtained 76,627 high quality sequences classified into 182 operational taxonomic units (OTUs). Firmicutes was the most common and diverse phylum (71.22% of sequences) with Streptococcus being the most common genus (49.72%). Known pathogens were found significantly more often in cases of infantile wheeze compared to controls, exemplified by Haemophilus spp. (OR=2.12, 95% Confidence Interval (CI) 1.82-2.47; P=5.46×10(-23)) and Staphylococcus spp. (OR=124.1, 95%CI 59.0-261.2; P=1.87×10(-241)). Other OTUs were less common in cases than controls, notably Veillonella spp. (OR=0.59, 95%CI=0.56-0.62; P=8.06×10(-86)).<h4>Discussion</h4>The airway microbiota appeared to contain many more Streptococci than found in Western Europe and the USA. Comparisons between healthy and wheezing infants revealed a significant difference in several bacterial phylotypes that were not confounded by antibiotics or use of inhaled steroids. The increased prevalence of pathogens such as Haemophilus and Staphylococcus spp. in cases may contribute to wheezing illnesses in this age group. |
| format | Article |
| id | doaj-art-e44bded46bfb4aac9908921504a0b459 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-e44bded46bfb4aac9908921504a0b4592025-08-20T02:30:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01710e4680310.1371/journal.pone.0046803Upper airways microbiota in antibiotic-naïve wheezing and healthy infants from the tropics of rural Ecuador.Paul Andres CardenasPhilip J CooperMichael J CoxMartha ChicoCarlos AriasMiriam F MoffattWilliam Osmond Cookson<h4>Background</h4>Observations that the airway microbiome is disturbed in asthma may be confounded by the widespread use of antibiotics and inhaled steroids. We have therefore examined the oropharyngeal microbiome in early onset wheezing infants from a rural area of tropical Ecuador where antibiotic usage is minimal and glucocorticoid usage is absent.<h4>Materials and methods</h4>We performed pyrosequencing of amplicons of the polymorphic bacterial 16S rRNA gene from oropharyngeal samples from 24 infants with non-infectious early onset wheezing and 24 healthy controls (average age 10.2 months). We analyzed microbial community structure and differences between cases and controls by QIIME software.<h4>Results</h4>We obtained 76,627 high quality sequences classified into 182 operational taxonomic units (OTUs). Firmicutes was the most common and diverse phylum (71.22% of sequences) with Streptococcus being the most common genus (49.72%). Known pathogens were found significantly more often in cases of infantile wheeze compared to controls, exemplified by Haemophilus spp. (OR=2.12, 95% Confidence Interval (CI) 1.82-2.47; P=5.46×10(-23)) and Staphylococcus spp. (OR=124.1, 95%CI 59.0-261.2; P=1.87×10(-241)). Other OTUs were less common in cases than controls, notably Veillonella spp. (OR=0.59, 95%CI=0.56-0.62; P=8.06×10(-86)).<h4>Discussion</h4>The airway microbiota appeared to contain many more Streptococci than found in Western Europe and the USA. Comparisons between healthy and wheezing infants revealed a significant difference in several bacterial phylotypes that were not confounded by antibiotics or use of inhaled steroids. The increased prevalence of pathogens such as Haemophilus and Staphylococcus spp. in cases may contribute to wheezing illnesses in this age group.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0046803&type=printable |
| spellingShingle | Paul Andres Cardenas Philip J Cooper Michael J Cox Martha Chico Carlos Arias Miriam F Moffatt William Osmond Cookson Upper airways microbiota in antibiotic-naïve wheezing and healthy infants from the tropics of rural Ecuador. PLoS ONE |
| title | Upper airways microbiota in antibiotic-naïve wheezing and healthy infants from the tropics of rural Ecuador. |
| title_full | Upper airways microbiota in antibiotic-naïve wheezing and healthy infants from the tropics of rural Ecuador. |
| title_fullStr | Upper airways microbiota in antibiotic-naïve wheezing and healthy infants from the tropics of rural Ecuador. |
| title_full_unstemmed | Upper airways microbiota in antibiotic-naïve wheezing and healthy infants from the tropics of rural Ecuador. |
| title_short | Upper airways microbiota in antibiotic-naïve wheezing and healthy infants from the tropics of rural Ecuador. |
| title_sort | upper airways microbiota in antibiotic naive wheezing and healthy infants from the tropics of rural ecuador |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0046803&type=printable |
| work_keys_str_mv | AT paulandrescardenas upperairwaysmicrobiotainantibioticnaivewheezingandhealthyinfantsfromthetropicsofruralecuador AT philipjcooper upperairwaysmicrobiotainantibioticnaivewheezingandhealthyinfantsfromthetropicsofruralecuador AT michaeljcox upperairwaysmicrobiotainantibioticnaivewheezingandhealthyinfantsfromthetropicsofruralecuador AT marthachico upperairwaysmicrobiotainantibioticnaivewheezingandhealthyinfantsfromthetropicsofruralecuador AT carlosarias upperairwaysmicrobiotainantibioticnaivewheezingandhealthyinfantsfromthetropicsofruralecuador AT miriamfmoffatt upperairwaysmicrobiotainantibioticnaivewheezingandhealthyinfantsfromthetropicsofruralecuador AT williamosmondcookson upperairwaysmicrobiotainantibioticnaivewheezingandhealthyinfantsfromthetropicsofruralecuador |