Systematic analysis of A-to-I RNA editing upon release of ADAR from the nucleolus
Adenosine-to-inosine (A-to-I) RNA editing, catalysed by two ADAR isoforms (p110 and p150) and ADARB1, is a critical regulatory step in gene expression. Intriguingly, the nucleolus is conspicuously rich in ADAR p110 and ADARB1, though the biological reason remains unclear. To investigate a putative r...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | RNA Biology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/15476286.2025.2515655 |
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| author | Ruben Lattuca Rümeyza Bascetin Vincent Detours Denis L. J. Lafontaine |
| author_facet | Ruben Lattuca Rümeyza Bascetin Vincent Detours Denis L. J. Lafontaine |
| author_sort | Ruben Lattuca |
| collection | DOAJ |
| description | Adenosine-to-inosine (A-to-I) RNA editing, catalysed by two ADAR isoforms (p110 and p150) and ADARB1, is a critical regulatory step in gene expression. Intriguingly, the nucleolus is conspicuously rich in ADAR p110 and ADARB1, though the biological reason remains unclear. To investigate a putative role of nucleolar enrichment in ADAR, we released it gradually from the nucleolus into the nucleoplasm by treating cells briefly with low doses of actinomycin D, known to disassemble the nucleolus. Deep sequencing of the transcriptome revealed that as ADAR dissociated from the nucleolus, RNA editing increased significantly, with sharp rises in both the number of edited sites and editing frequency. This co-transcriptional editing, predominantly in intronic regions, was associated with disrupted pre-mRNA splicing, causing exon skipping and intron retention which remodelled gene expression. These findings suggest that the nucleolar localization of ADAR serves to restrain its activity, preventing excessive editing that could lead to splicing errors and cellular dysfunction. |
| format | Article |
| id | doaj-art-e43f948ca03445c0b49ab1fb57827675 |
| institution | DOAJ |
| issn | 1547-6286 1555-8584 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | RNA Biology |
| spelling | doaj-art-e43f948ca03445c0b49ab1fb578276752025-08-20T03:16:31ZengTaylor & Francis GroupRNA Biology1547-62861555-85842025-12-0122111810.1080/15476286.2025.2515655Systematic analysis of A-to-I RNA editing upon release of ADAR from the nucleolusRuben Lattuca0Rümeyza Bascetin1Vincent Detours2Denis L. J. Lafontaine3RNA Molecular Biology, Fonds de la Recherche Scientifique (F.R.S./FNRS), Université libre de Bruxelles (ULB), Biopark campus, B-6041 Gosselies, BelgiumRNA Molecular Biology, Fonds de la Recherche Scientifique (F.R.S./FNRS), Université libre de Bruxelles (ULB), Biopark campus, B-6041 Gosselies, BelgiumIRIBHM Jacques E. Dumont, Université libre de Bruxelles, Lennik, BelgiumRNA Molecular Biology, Fonds de la Recherche Scientifique (F.R.S./FNRS), Université libre de Bruxelles (ULB), Biopark campus, B-6041 Gosselies, BelgiumAdenosine-to-inosine (A-to-I) RNA editing, catalysed by two ADAR isoforms (p110 and p150) and ADARB1, is a critical regulatory step in gene expression. Intriguingly, the nucleolus is conspicuously rich in ADAR p110 and ADARB1, though the biological reason remains unclear. To investigate a putative role of nucleolar enrichment in ADAR, we released it gradually from the nucleolus into the nucleoplasm by treating cells briefly with low doses of actinomycin D, known to disassemble the nucleolus. Deep sequencing of the transcriptome revealed that as ADAR dissociated from the nucleolus, RNA editing increased significantly, with sharp rises in both the number of edited sites and editing frequency. This co-transcriptional editing, predominantly in intronic regions, was associated with disrupted pre-mRNA splicing, causing exon skipping and intron retention which remodelled gene expression. These findings suggest that the nucleolar localization of ADAR serves to restrain its activity, preventing excessive editing that could lead to splicing errors and cellular dysfunction.https://www.tandfonline.com/doi/10.1080/15476286.2025.2515655A-to-I RNA editingADARnucleolusRNA modificationepitranscriptomicspre-mRNA splicing regulation |
| spellingShingle | Ruben Lattuca Rümeyza Bascetin Vincent Detours Denis L. J. Lafontaine Systematic analysis of A-to-I RNA editing upon release of ADAR from the nucleolus RNA Biology A-to-I RNA editing ADAR nucleolus RNA modification epitranscriptomics pre-mRNA splicing regulation |
| title | Systematic analysis of A-to-I RNA editing upon release of ADAR from the nucleolus |
| title_full | Systematic analysis of A-to-I RNA editing upon release of ADAR from the nucleolus |
| title_fullStr | Systematic analysis of A-to-I RNA editing upon release of ADAR from the nucleolus |
| title_full_unstemmed | Systematic analysis of A-to-I RNA editing upon release of ADAR from the nucleolus |
| title_short | Systematic analysis of A-to-I RNA editing upon release of ADAR from the nucleolus |
| title_sort | systematic analysis of a to i rna editing upon release of adar from the nucleolus |
| topic | A-to-I RNA editing ADAR nucleolus RNA modification epitranscriptomics pre-mRNA splicing regulation |
| url | https://www.tandfonline.com/doi/10.1080/15476286.2025.2515655 |
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