The Effectiveness of Bandotan (Ageratum conyzoides L.) Leaf Extract on Mortality of Boophilus microplus In Vitro

The purpose of this research is to find the effectiveness of bandotan's (Ageratum conyzoides L.) leaf extract on mortality of Boophilus microplus in vitro and also to get of lethal concentration and lethal time. This research used 125 Boophilus microplus. This research used five treatments, nam...

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Main Authors: Dwi Retno Widyawati, Iwan Sahrial Hamid, Moh. Sukmanadi, Sri Agus Sudjarwo, Muhammad Hambal
Format: Article
Language:English
Published: Universitas Airlangga 2021-09-01
Series:Journal of Parasite Science
Subjects:
Online Access:https://e-journal.unair.ac.id/JoPS/article/view/29959
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author Dwi Retno Widyawati
Iwan Sahrial Hamid
Moh. Sukmanadi
Sri Agus Sudjarwo
Muhammad Hambal
author_facet Dwi Retno Widyawati
Iwan Sahrial Hamid
Moh. Sukmanadi
Sri Agus Sudjarwo
Muhammad Hambal
author_sort Dwi Retno Widyawati
collection DOAJ
description The purpose of this research is to find the effectiveness of bandotan's (Ageratum conyzoides L.) leaf extract on mortality of Boophilus microplus in vitro and also to get of lethal concentration and lethal time. This research used 125 Boophilus microplus. This research used five treatments, namely neguvon suspensionon (K+), ethanol 1% (K-), 1% concentration (P1), 2% concentration (P2) and 3% concentration (P3). The observation of mortality was done every six hours. Boophilus microplus dies when there is no movement. The data were analyzed using one-way ANOVA and followed by Duncan's Multiple Distance Test. The result is said to be significant if p<0.05. Probit analysis of LC50 is 1.63% at the first hour, 0.85% at the second hour, 0.54% at the third hour, 0.34% at the fourth hour, 0.04% at the fifth hour and 0.04% at the sixth hour, LC90 reached by using 5.6%, 2.9%, 1.8%, 1.2%, 0.16 and 0.16% concentration in 1, 2, 3, 4, 5 and 6 hours, respectively. The LT50 of ethanol 1% (K-) was at 10.142 hours, neguvon suspension on (K+) was at 0.747 hours, 1% concentration (P1) was at 2.184 hours, 2% concentration (P2) was at 1.579 hours, 3% concentration (P3) was at 0.923 hours and LT90 was at 23.734, 1.749, 5.110, 3.695 and 2.159 hours. The conclusion of this study was bandotan's (Ageratum conyzoides L.) leaf extract was effective as an acaricide based on the mortality of Boophilus microplus.
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spelling doaj-art-e435c987d1f74320a3773ec4f6b390ef2025-08-20T02:44:29ZengUniversitas AirlanggaJournal of Parasite Science2599-09932656-53312021-09-0151151810.20473/jops.v5i1.2995924515The Effectiveness of Bandotan (Ageratum conyzoides L.) Leaf Extract on Mortality of Boophilus microplus In VitroDwi Retno Widyawati0Iwan Sahrial Hamid1Moh. Sukmanadi2Sri Agus Sudjarwo3Muhammad Hambal4Student, Faculty of Veterinary Medicine, Universitas AirlanggaDivision of Veterinary Basic, Faculty of Veterinary Medicine, Universitas AirlanggaDivision of Veterinary Basic, Faculty of Veterinary Medicine, Universitas AirlanggaDivision of Veterinary Basic, Faculty of Veterinary Medicine, Universitas AirlanggaDepartment of Veterinary Parasitology, Faculty of Veterinary Medicine, Universitas Syiah KualaThe purpose of this research is to find the effectiveness of bandotan's (Ageratum conyzoides L.) leaf extract on mortality of Boophilus microplus in vitro and also to get of lethal concentration and lethal time. This research used 125 Boophilus microplus. This research used five treatments, namely neguvon suspensionon (K+), ethanol 1% (K-), 1% concentration (P1), 2% concentration (P2) and 3% concentration (P3). The observation of mortality was done every six hours. Boophilus microplus dies when there is no movement. The data were analyzed using one-way ANOVA and followed by Duncan's Multiple Distance Test. The result is said to be significant if p<0.05. Probit analysis of LC50 is 1.63% at the first hour, 0.85% at the second hour, 0.54% at the third hour, 0.34% at the fourth hour, 0.04% at the fifth hour and 0.04% at the sixth hour, LC90 reached by using 5.6%, 2.9%, 1.8%, 1.2%, 0.16 and 0.16% concentration in 1, 2, 3, 4, 5 and 6 hours, respectively. The LT50 of ethanol 1% (K-) was at 10.142 hours, neguvon suspension on (K+) was at 0.747 hours, 1% concentration (P1) was at 2.184 hours, 2% concentration (P2) was at 1.579 hours, 3% concentration (P3) was at 0.923 hours and LT90 was at 23.734, 1.749, 5.110, 3.695 and 2.159 hours. The conclusion of this study was bandotan's (Ageratum conyzoides L.) leaf extract was effective as an acaricide based on the mortality of Boophilus microplus.https://e-journal.unair.ac.id/JoPS/article/view/29959ageratum conyzoidesboophilus microplusleaf extractneguvonprobit analysis
spellingShingle Dwi Retno Widyawati
Iwan Sahrial Hamid
Moh. Sukmanadi
Sri Agus Sudjarwo
Muhammad Hambal
The Effectiveness of Bandotan (Ageratum conyzoides L.) Leaf Extract on Mortality of Boophilus microplus In Vitro
Journal of Parasite Science
ageratum conyzoides
boophilus microplus
leaf extract
neguvon
probit analysis
title The Effectiveness of Bandotan (Ageratum conyzoides L.) Leaf Extract on Mortality of Boophilus microplus In Vitro
title_full The Effectiveness of Bandotan (Ageratum conyzoides L.) Leaf Extract on Mortality of Boophilus microplus In Vitro
title_fullStr The Effectiveness of Bandotan (Ageratum conyzoides L.) Leaf Extract on Mortality of Boophilus microplus In Vitro
title_full_unstemmed The Effectiveness of Bandotan (Ageratum conyzoides L.) Leaf Extract on Mortality of Boophilus microplus In Vitro
title_short The Effectiveness of Bandotan (Ageratum conyzoides L.) Leaf Extract on Mortality of Boophilus microplus In Vitro
title_sort effectiveness of bandotan ageratum conyzoides l leaf extract on mortality of boophilus microplus in vitro
topic ageratum conyzoides
boophilus microplus
leaf extract
neguvon
probit analysis
url https://e-journal.unair.ac.id/JoPS/article/view/29959
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