Cannabidiol-Based Thiosemicarbazones: A Preliminary Study Evaluating Their Anti-Tyrosinase Properties

Cannabidiol (CBD), a non-psychoactive cannabinoid, has attracted significant research interest due to its antioxidant, anti-inflammatory, and neuroprotective properties. As a versatile scaffold in drug discovery, CBD has been widely explored for developing novel therapeutics. In this study, we synth...

Full description

Saved in:
Bibliographic Details
Main Authors: Eliav Peretz, Noa Ashkenazi, Sanaa Musa
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Molecules
Subjects:
Online Access:https://www.mdpi.com/1420-3049/30/6/1291
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849341240016896000
author Eliav Peretz
Noa Ashkenazi
Sanaa Musa
author_facet Eliav Peretz
Noa Ashkenazi
Sanaa Musa
author_sort Eliav Peretz
collection DOAJ
description Cannabidiol (CBD), a non-psychoactive cannabinoid, has attracted significant research interest due to its antioxidant, anti-inflammatory, and neuroprotective properties. As a versatile scaffold in drug discovery, CBD has been widely explored for developing novel therapeutics. In this study, we synthesized and evaluated the anti-tyrosinase activity of CBD-based thiosemicarbazones. Structure–activity relationship (SAR) analyses were conducted to assess the impact of various functional groups on tyrosinase inhibition, including an evaluation of inhibitory kinetics for selected compounds. The synthesized derivatives demonstrated potent tyrosinase inhibition, with activity comparable to kojic acid, a standard tyrosinase inhibitor. Given the crucial role of tyrosinase in melanin biosynthesis, these findings suggest that CBD-based thiosemicarbazones could serve as promising candidates for managing tyrosinase-related disorders, including hyperpigmentation and melanogenesis-related conditions. Moreover, the presence of thiosemicarbazone moieties may contribute to the observed inhibitory effects, potentially through metal chelation at the enzyme’s active site. This study provides valuable insights into the design of CBD-derived inhibitors targeting tyrosinase. Further optimization and in-depth biological evaluation are warranted to explore their full therapeutic potential.
format Article
id doaj-art-e433e35b2fe14387a7d58b3761379315
institution Kabale University
issn 1420-3049
language English
publishDate 2025-03-01
publisher MDPI AG
record_format Article
series Molecules
spelling doaj-art-e433e35b2fe14387a7d58b37613793152025-08-20T03:43:40ZengMDPI AGMolecules1420-30492025-03-01306129110.3390/molecules30061291Cannabidiol-Based Thiosemicarbazones: A Preliminary Study Evaluating Their Anti-Tyrosinase PropertiesEliav Peretz0Noa Ashkenazi1Sanaa Musa2Department of Biotechnology, Tel-Hai Academic College, Kiryat Shmona 11016, IsraelDepartment of Biotechnology, Tel-Hai Academic College, Kiryat Shmona 11016, IsraelDepartment of Biotechnology, Tel-Hai Academic College, Kiryat Shmona 11016, IsraelCannabidiol (CBD), a non-psychoactive cannabinoid, has attracted significant research interest due to its antioxidant, anti-inflammatory, and neuroprotective properties. As a versatile scaffold in drug discovery, CBD has been widely explored for developing novel therapeutics. In this study, we synthesized and evaluated the anti-tyrosinase activity of CBD-based thiosemicarbazones. Structure–activity relationship (SAR) analyses were conducted to assess the impact of various functional groups on tyrosinase inhibition, including an evaluation of inhibitory kinetics for selected compounds. The synthesized derivatives demonstrated potent tyrosinase inhibition, with activity comparable to kojic acid, a standard tyrosinase inhibitor. Given the crucial role of tyrosinase in melanin biosynthesis, these findings suggest that CBD-based thiosemicarbazones could serve as promising candidates for managing tyrosinase-related disorders, including hyperpigmentation and melanogenesis-related conditions. Moreover, the presence of thiosemicarbazone moieties may contribute to the observed inhibitory effects, potentially through metal chelation at the enzyme’s active site. This study provides valuable insights into the design of CBD-derived inhibitors targeting tyrosinase. Further optimization and in-depth biological evaluation are warranted to explore their full therapeutic potential.https://www.mdpi.com/1420-3049/30/6/1291cannabidiolCBD derivativesthiosemicarbazonesanti-tyrosinaseantioxidant activities
spellingShingle Eliav Peretz
Noa Ashkenazi
Sanaa Musa
Cannabidiol-Based Thiosemicarbazones: A Preliminary Study Evaluating Their Anti-Tyrosinase Properties
Molecules
cannabidiol
CBD derivatives
thiosemicarbazones
anti-tyrosinase
antioxidant activities
title Cannabidiol-Based Thiosemicarbazones: A Preliminary Study Evaluating Their Anti-Tyrosinase Properties
title_full Cannabidiol-Based Thiosemicarbazones: A Preliminary Study Evaluating Their Anti-Tyrosinase Properties
title_fullStr Cannabidiol-Based Thiosemicarbazones: A Preliminary Study Evaluating Their Anti-Tyrosinase Properties
title_full_unstemmed Cannabidiol-Based Thiosemicarbazones: A Preliminary Study Evaluating Their Anti-Tyrosinase Properties
title_short Cannabidiol-Based Thiosemicarbazones: A Preliminary Study Evaluating Their Anti-Tyrosinase Properties
title_sort cannabidiol based thiosemicarbazones a preliminary study evaluating their anti tyrosinase properties
topic cannabidiol
CBD derivatives
thiosemicarbazones
anti-tyrosinase
antioxidant activities
url https://www.mdpi.com/1420-3049/30/6/1291
work_keys_str_mv AT eliavperetz cannabidiolbasedthiosemicarbazonesapreliminarystudyevaluatingtheirantityrosinaseproperties
AT noaashkenazi cannabidiolbasedthiosemicarbazonesapreliminarystudyevaluatingtheirantityrosinaseproperties
AT sanaamusa cannabidiolbasedthiosemicarbazonesapreliminarystudyevaluatingtheirantityrosinaseproperties