Development and Validation of a Rapid and Sensitive Ultrahigh-Performance Liquid Chromatography with Diode Array Detection Method for Quantification of Sertraline in Pharmaceutical Formulations
Objective: This study aimed to develop and validate a new, sensitive, fast, and simple ultrahighperformance liquid chromatography (UHPLC) method for the quantification of sertraline in pharmaceutical preparations and then apply it to commercial pharmaceutical preparations.Methods: Ultrahigh-performa...
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Atatürk University
2024-01-01
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| Series: | Pharmata |
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| Online Access: | https://dergipark.org.tr/en/download/article-file/3683456 |
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| author | Tuğrul Çağrı Akman Mahmut Can Bebek |
| author_facet | Tuğrul Çağrı Akman Mahmut Can Bebek |
| author_sort | Tuğrul Çağrı Akman |
| collection | DOAJ |
| description | Objective: This study aimed to develop and validate a new, sensitive, fast, and simple ultrahighperformance liquid chromatography (UHPLC) method for the quantification of sertraline in pharmaceutical preparations and then apply it to commercial pharmaceutical preparations.Methods: Ultrahigh-performance liquid chromatography with diode array detection was used in the study. In the method, methanol and deionized water containing 0.05% TFA (70:30, v/v) were used as the mobile phase, and the C18 column was used as the stationary phase. The flow rate was 1.0 mL/min, the injection volume was 20 μL, and the column temperature was 40°C. The detector was set at 254 nm.Results: The retention time of sertraline was 2.1 minutes, and the analysis time of the method was 4 minutes. The equation of the calibration curve was determined as y=0.1096x − 0.0156 (R 0.9997). While the limit of detection and limit of quantitation values were 0.1 and 0.3 µg/mL, respectively, the method showed linearity between concentrations of 0.3 and 100 µg/mL. The analytical recovery of the method from the pharmaceutical preparation was within accepted limits (98%-102%). In addition, the relative error percentage and relative standard deviation values, which express the accuracy and precision parameters, respectively, were found to be less than 8%.Conclusion: In the study, a UHPLC method with high sensitivity and a wide working range was developed for the quantification of sertraline in a short analysis time. As a result, the applicability of the developed method to pharmaceutical preparations has also been proven. |
| format | Article |
| id | doaj-art-e433a2e35c8e4dddacf9a79ceb1c4b4b |
| institution | OA Journals |
| issn | 2980-1966 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Atatürk University |
| record_format | Article |
| series | Pharmata |
| spelling | doaj-art-e433a2e35c8e4dddacf9a79ceb1c4b4b2025-08-20T01:57:15ZengAtatürk UniversityPharmata2980-19662024-01-014171310.5152/Pharmata.2024.2302855Development and Validation of a Rapid and Sensitive Ultrahigh-Performance Liquid Chromatography with Diode Array Detection Method for Quantification of Sertraline in Pharmaceutical FormulationsTuğrul Çağrı Akman0https://orcid.org/0000-0002-5439-950XMahmut Can Bebekhttps://orcid.org/0000-0003-3994-073XErzincan Binali Yıldırım Üniversitesi Eczacılık FakültesiObjective: This study aimed to develop and validate a new, sensitive, fast, and simple ultrahighperformance liquid chromatography (UHPLC) method for the quantification of sertraline in pharmaceutical preparations and then apply it to commercial pharmaceutical preparations.Methods: Ultrahigh-performance liquid chromatography with diode array detection was used in the study. In the method, methanol and deionized water containing 0.05% TFA (70:30, v/v) were used as the mobile phase, and the C18 column was used as the stationary phase. The flow rate was 1.0 mL/min, the injection volume was 20 μL, and the column temperature was 40°C. The detector was set at 254 nm.Results: The retention time of sertraline was 2.1 minutes, and the analysis time of the method was 4 minutes. The equation of the calibration curve was determined as y=0.1096x − 0.0156 (R 0.9997). While the limit of detection and limit of quantitation values were 0.1 and 0.3 µg/mL, respectively, the method showed linearity between concentrations of 0.3 and 100 µg/mL. The analytical recovery of the method from the pharmaceutical preparation was within accepted limits (98%-102%). In addition, the relative error percentage and relative standard deviation values, which express the accuracy and precision parameters, respectively, were found to be less than 8%.Conclusion: In the study, a UHPLC method with high sensitivity and a wide working range was developed for the quantification of sertraline in a short analysis time. As a result, the applicability of the developed method to pharmaceutical preparations has also been proven.https://dergipark.org.tr/en/download/article-file/3683456pharmaceutical dosage formssertralineuhplc-dadvalidationpharmaceutical dosage formssertralineuhplc-dadvalidation |
| spellingShingle | Tuğrul Çağrı Akman Mahmut Can Bebek Development and Validation of a Rapid and Sensitive Ultrahigh-Performance Liquid Chromatography with Diode Array Detection Method for Quantification of Sertraline in Pharmaceutical Formulations Pharmata pharmaceutical dosage forms sertraline uhplc-dad validation pharmaceutical dosage forms sertraline uhplc-dad validation |
| title | Development and Validation of a Rapid and Sensitive Ultrahigh-Performance Liquid Chromatography with Diode Array Detection Method for Quantification of Sertraline in Pharmaceutical Formulations |
| title_full | Development and Validation of a Rapid and Sensitive Ultrahigh-Performance Liquid Chromatography with Diode Array Detection Method for Quantification of Sertraline in Pharmaceutical Formulations |
| title_fullStr | Development and Validation of a Rapid and Sensitive Ultrahigh-Performance Liquid Chromatography with Diode Array Detection Method for Quantification of Sertraline in Pharmaceutical Formulations |
| title_full_unstemmed | Development and Validation of a Rapid and Sensitive Ultrahigh-Performance Liquid Chromatography with Diode Array Detection Method for Quantification of Sertraline in Pharmaceutical Formulations |
| title_short | Development and Validation of a Rapid and Sensitive Ultrahigh-Performance Liquid Chromatography with Diode Array Detection Method for Quantification of Sertraline in Pharmaceutical Formulations |
| title_sort | development and validation of a rapid and sensitive ultrahigh performance liquid chromatography with diode array detection method for quantification of sertraline in pharmaceutical formulations |
| topic | pharmaceutical dosage forms sertraline uhplc-dad validation pharmaceutical dosage forms sertraline uhplc-dad validation |
| url | https://dergipark.org.tr/en/download/article-file/3683456 |
| work_keys_str_mv | AT tugrulcagrıakman developmentandvalidationofarapidandsensitiveultrahighperformanceliquidchromatographywithdiodearraydetectionmethodforquantificationofsertralineinpharmaceuticalformulations AT mahmutcanbebek developmentandvalidationofarapidandsensitiveultrahighperformanceliquidchromatographywithdiodearraydetectionmethodforquantificationofsertralineinpharmaceuticalformulations |