Determination of riboflavin in dragee “Revit” by HPLC
The aim of the work is development of express, selective, reproducible methods for the determination of riboflavin by HPLC in a multivitamin preparation, dragee “Revit”. Materials and methods. Degasser, binary pump, autosampler, thermostated column compartment, diode array detector. Chromatograph...
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Zaporizhzhia State Medical and Pharmaceutical University
2022-08-01
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| Series: | Aktualʹnì Pitannâ Farmacevtičnoï ì Medičnoï Nauki ta Praktiki |
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| Online Access: | http://pharmed.zsmu.edu.ua/article/view/258888/258739 |
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| author | B. О. Varynskyi M. Yu. Zeleniuk A. H. Kaplaushenko |
| author_facet | B. О. Varynskyi M. Yu. Zeleniuk A. H. Kaplaushenko |
| author_sort | B. О. Varynskyi |
| collection | DOAJ |
| description | The aim of the work is development of express, selective, reproducible methods for the determination of riboflavin by HPLC in a multivitamin preparation, dragee “Revit”.
Materials and methods. Degasser, binary pump, autosampler, thermostated column compartment, diode array detector. Chromatographic column Zorbax SB-C18, 30 mm × 4.6 mm, 1.8 µm. Samples were prepared using analytical electronic scales Kern ABT 100-5M, ultrasonic bath Ultrasonic XUBA 3, nylon ultrafilters 0.12 µm.
Results. 0.1 % formic acid was selected as a buffer solution in the eluent. The maximum values of logD were observed at intervals for riboflavin at pH from 2 to 4. Therefore, the maximum retention of the riboflavin was observed when using the eluent, рН which corresponds to this interval. The pH value for 0.1 % formic acid solution was 2.7. Therefore, such a buffer was chosen by us as part of the eluent. Acetonitrile was selected as the organic modifier. The optimal content of the compound was observed at 10 % acetonitrile.
Conclusions. The chromatographic behavior of riboflavin on the basis of chemical-analytical properties was studied. The optimal conditions for chromatographic determination were determined and a specific, express method for the determination of riboflavin in “Revit” dragee was developed. The technique on a real sample of “Revit” dragee was elaborated. The method was recommended for use in control and analytical and research laboratories. |
| format | Article |
| id | doaj-art-e42e22a25d7d42f28b539970ab9ef5a2 |
| institution | DOAJ |
| issn | 2306-8094 2409-2932 |
| language | English |
| publishDate | 2022-08-01 |
| publisher | Zaporizhzhia State Medical and Pharmaceutical University |
| record_format | Article |
| series | Aktualʹnì Pitannâ Farmacevtičnoï ì Medičnoï Nauki ta Praktiki |
| spelling | doaj-art-e42e22a25d7d42f28b539970ab9ef5a22025-08-20T03:19:08ZengZaporizhzhia State Medical and Pharmaceutical UniversityAktualʹnì Pitannâ Farmacevtičnoï ì Medičnoï Nauki ta Praktiki2306-80942409-29322022-08-0115214014410.14739/2409-2932.2022.2.258888Determination of riboflavin in dragee “Revit” by HPLCB. О. Varynskyi0https://orcid.org/0000-0002-1551-8879M. Yu. Zeleniuk1A. H. Kaplaushenko2https://orcid.org/0000-0003-3704-5539Zaporizhzhia State Medical University, UkraineZaporizhzhia State Medical University, UkraineZaporizhzhia State Medical University, UkraineThe aim of the work is development of express, selective, reproducible methods for the determination of riboflavin by HPLC in a multivitamin preparation, dragee “Revit”. Materials and methods. Degasser, binary pump, autosampler, thermostated column compartment, diode array detector. Chromatographic column Zorbax SB-C18, 30 mm × 4.6 mm, 1.8 µm. Samples were prepared using analytical electronic scales Kern ABT 100-5M, ultrasonic bath Ultrasonic XUBA 3, nylon ultrafilters 0.12 µm. Results. 0.1 % formic acid was selected as a buffer solution in the eluent. The maximum values of logD were observed at intervals for riboflavin at pH from 2 to 4. Therefore, the maximum retention of the riboflavin was observed when using the eluent, рН which corresponds to this interval. The pH value for 0.1 % formic acid solution was 2.7. Therefore, such a buffer was chosen by us as part of the eluent. Acetonitrile was selected as the organic modifier. The optimal content of the compound was observed at 10 % acetonitrile. Conclusions. The chromatographic behavior of riboflavin on the basis of chemical-analytical properties was studied. The optimal conditions for chromatographic determination were determined and a specific, express method for the determination of riboflavin in “Revit” dragee was developed. The technique on a real sample of “Revit” dragee was elaborated. The method was recommended for use in control and analytical and research laboratories.http://pharmed.zsmu.edu.ua/article/view/258888/258739riboflavindrageevitaminsanalysisliquid chromatographyhplc |
| spellingShingle | B. О. Varynskyi M. Yu. Zeleniuk A. H. Kaplaushenko Determination of riboflavin in dragee “Revit” by HPLC Aktualʹnì Pitannâ Farmacevtičnoï ì Medičnoï Nauki ta Praktiki riboflavin dragee vitamins analysis liquid chromatography hplc |
| title | Determination of riboflavin in dragee “Revit” by HPLC |
| title_full | Determination of riboflavin in dragee “Revit” by HPLC |
| title_fullStr | Determination of riboflavin in dragee “Revit” by HPLC |
| title_full_unstemmed | Determination of riboflavin in dragee “Revit” by HPLC |
| title_short | Determination of riboflavin in dragee “Revit” by HPLC |
| title_sort | determination of riboflavin in dragee revit by hplc |
| topic | riboflavin dragee vitamins analysis liquid chromatography hplc |
| url | http://pharmed.zsmu.edu.ua/article/view/258888/258739 |
| work_keys_str_mv | AT bovarynskyi determinationofriboflavinindrageerevitbyhplc AT myuzeleniuk determinationofriboflavinindrageerevitbyhplc AT ahkaplaushenko determinationofriboflavinindrageerevitbyhplc |