Oxidative stress-generated antibacterial and anticancer activities of piperine incorporated guar gum and psyllium husk derived biopolymeric nanocomposite
The synthesis of guar gum and psyllium husk mucilage-mediated piperine nanocomposite was reported in order to develop a modified phytochemical-based nanomaterial with potent antimicrobial and anticancer properties. Piperine was nanonized by entrapping within guar gum and psyllium husk mucilage-deriv...
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Elsevier
2025-08-01
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| Series: | Chemistry of Inorganic Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2949746925000187 |
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| author | Sanghita Das Debojyoti De Debbethi Bera Parimal Karmakar Sukhen Das Anindita Dey |
| author_facet | Sanghita Das Debojyoti De Debbethi Bera Parimal Karmakar Sukhen Das Anindita Dey |
| author_sort | Sanghita Das |
| collection | DOAJ |
| description | The synthesis of guar gum and psyllium husk mucilage-mediated piperine nanocomposite was reported in order to develop a modified phytochemical-based nanomaterial with potent antimicrobial and anticancer properties. Piperine was nanonized by entrapping within guar gum and psyllium husk mucilage-derived nanocomposite. We have taken guar gum and husk mucilage in three different ratios (1:1, 2:1, 1:2) to formulate nanocomposites for comparative study. Highest loading percentage of piperine in nanocomposite was found to be 83 ± 1.2 % which indicated excellent incorporation efficiency. The release kinetics of piperine from nanocomposites in acidic pH showed sustained but faster release behaviour than physiological pH which confirms the availability of adequate amount of therapeutics at the site of anticancer application. The as-prepared nanocomposite showed strong oxidative stress mediated antimicrobial activities against Gram-positive Staphylococcus aureus and Gram-negative Pseudomonas aeruginosa. Lowest IC50 value of nanocomposite i.e. 3.14 mg/mL against the adenocarcinomic human alveolar basal epithelial cell line were evaluated where the effective concentration of piperine was significantly low (76.3 μg/mL). The biocompatibility study of the nanocomposite was performed against human lung fibroblast cell line where up to 88.3 % cell survivability was observed. Moreover, the nanocomposite had the potential to induce oxidative stress, for which significant mortality of bacterial and cancer cells was detected. |
| format | Article |
| id | doaj-art-e427da561b8a4e208a13d616b5d914dd |
| institution | DOAJ |
| issn | 2949-7469 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
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| series | Chemistry of Inorganic Materials |
| spelling | doaj-art-e427da561b8a4e208a13d616b5d914dd2025-08-20T02:55:19ZengElsevierChemistry of Inorganic Materials2949-74692025-08-01610010410.1016/j.cinorg.2025.100104Oxidative stress-generated antibacterial and anticancer activities of piperine incorporated guar gum and psyllium husk derived biopolymeric nanocompositeSanghita Das0Debojyoti De1Debbethi Bera2Parimal Karmakar3Sukhen Das4Anindita Dey5Department of Physics, Jadavpur University, Kolkata, 700032, India; Department of Botany, Asutosh College, Kolkata, 700026, IndiaDepartment of Life Science and Biotechnology, Jadavpur University, Kolkata, 700032, IndiaDepartment of Physics, Jadavpur University, Kolkata, 700032, India; Centre for Interdisciplinary Research and Education, 404B, Jodhpur Park, Kolkata, 700068, IndiaDepartment of Life Science and Biotechnology, Jadavpur University, Kolkata, 700032, IndiaDepartment of Physics, Jadavpur University, Kolkata, 700032, India; Corresponding author.Department of Botany, Asutosh College, Kolkata, 700026, India; Centre for Interdisciplinary Research and Education, 404B, Jodhpur Park, Kolkata, 700068, India; Corresponding author. Department of Botany, Asutosh College, Kolkata, 700026, India.The synthesis of guar gum and psyllium husk mucilage-mediated piperine nanocomposite was reported in order to develop a modified phytochemical-based nanomaterial with potent antimicrobial and anticancer properties. Piperine was nanonized by entrapping within guar gum and psyllium husk mucilage-derived nanocomposite. We have taken guar gum and husk mucilage in three different ratios (1:1, 2:1, 1:2) to formulate nanocomposites for comparative study. Highest loading percentage of piperine in nanocomposite was found to be 83 ± 1.2 % which indicated excellent incorporation efficiency. The release kinetics of piperine from nanocomposites in acidic pH showed sustained but faster release behaviour than physiological pH which confirms the availability of adequate amount of therapeutics at the site of anticancer application. The as-prepared nanocomposite showed strong oxidative stress mediated antimicrobial activities against Gram-positive Staphylococcus aureus and Gram-negative Pseudomonas aeruginosa. Lowest IC50 value of nanocomposite i.e. 3.14 mg/mL against the adenocarcinomic human alveolar basal epithelial cell line were evaluated where the effective concentration of piperine was significantly low (76.3 μg/mL). The biocompatibility study of the nanocomposite was performed against human lung fibroblast cell line where up to 88.3 % cell survivability was observed. Moreover, the nanocomposite had the potential to induce oxidative stress, for which significant mortality of bacterial and cancer cells was detected.http://www.sciencedirect.com/science/article/pii/S2949746925000187Psyllium husk-guar gum bio-nanocompositePiperineSustained releaseAntibacterial activityOxidative stressAnticancer activity |
| spellingShingle | Sanghita Das Debojyoti De Debbethi Bera Parimal Karmakar Sukhen Das Anindita Dey Oxidative stress-generated antibacterial and anticancer activities of piperine incorporated guar gum and psyllium husk derived biopolymeric nanocomposite Chemistry of Inorganic Materials Psyllium husk-guar gum bio-nanocomposite Piperine Sustained release Antibacterial activity Oxidative stress Anticancer activity |
| title | Oxidative stress-generated antibacterial and anticancer activities of piperine incorporated guar gum and psyllium husk derived biopolymeric nanocomposite |
| title_full | Oxidative stress-generated antibacterial and anticancer activities of piperine incorporated guar gum and psyllium husk derived biopolymeric nanocomposite |
| title_fullStr | Oxidative stress-generated antibacterial and anticancer activities of piperine incorporated guar gum and psyllium husk derived biopolymeric nanocomposite |
| title_full_unstemmed | Oxidative stress-generated antibacterial and anticancer activities of piperine incorporated guar gum and psyllium husk derived biopolymeric nanocomposite |
| title_short | Oxidative stress-generated antibacterial and anticancer activities of piperine incorporated guar gum and psyllium husk derived biopolymeric nanocomposite |
| title_sort | oxidative stress generated antibacterial and anticancer activities of piperine incorporated guar gum and psyllium husk derived biopolymeric nanocomposite |
| topic | Psyllium husk-guar gum bio-nanocomposite Piperine Sustained release Antibacterial activity Oxidative stress Anticancer activity |
| url | http://www.sciencedirect.com/science/article/pii/S2949746925000187 |
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