Optimization of DNase I Removal of Contaminating DNA from RNA for Use in Quantitative RNA-PCR
In competitive RNA-PCR studies, contaminating DNA can produce incorrect results because of its potential to act as a second competitor. Preliminary studies using published methods for DNase I digestion of DNA as a contaminant of RNA, followed by thermal inactivation of the enzyme at 95°C for 5 min b...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1996-06-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/96206st02 |
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