The potential biofortification role of Actinopolyspora sp. JTT-01 in enhancing the yield and tissue chemical composition of caraway plants

Abstract The need for improving plant production, nutritional value, and medicinal applications has become increasingly important due to the growing global population. The caraway (Carum carvi L) plant has been recognized for its broad range of nutritional and therapeutic uses. Consequently, this st...

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Main Authors: Ahmed M. Mahmoud, Mohamed S. Sheteiwy, Ali El-Keblawy, Zaid Ulhassan, Maha H. Khalaf, Hussein S. Mohamed, Mohammad K. Okla, Amal Mohamed AlGarawi, Ahmed M. El-Sawah, Enas S. Ahmed, Ahmed M. Reyad
Format: Article
Language:English
Published: BMC 2025-04-01
Series:BMC Plant Biology
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Online Access:https://doi.org/10.1186/s12870-025-06137-1
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author Ahmed M. Mahmoud
Mohamed S. Sheteiwy
Ali El-Keblawy
Zaid Ulhassan
Maha H. Khalaf
Hussein S. Mohamed
Mohammad K. Okla
Amal Mohamed AlGarawi
Ahmed M. El-Sawah
Enas S. Ahmed
Ahmed M. Reyad
author_facet Ahmed M. Mahmoud
Mohamed S. Sheteiwy
Ali El-Keblawy
Zaid Ulhassan
Maha H. Khalaf
Hussein S. Mohamed
Mohammad K. Okla
Amal Mohamed AlGarawi
Ahmed M. El-Sawah
Enas S. Ahmed
Ahmed M. Reyad
author_sort Ahmed M. Mahmoud
collection DOAJ
description Abstract The need for improving plant production, nutritional value, and medicinal applications has become increasingly important due to the growing global population. The caraway (Carum carvi L) plant has been recognized for its broad range of nutritional and therapeutic uses. Consequently, this study aimed to increase caraway seeds’ nutritional and biological value. To achieve this, the Actinopolyspora sp. JTT-0 strain, isolated from the medicinal plant Tephrosia purpurea, was investigated for its potential biofortification role to enhance caraway yield and quality. Our results revealed significant improvements (p < 0.05) in various physical parameters, such as seed yield, pod length, and bulk density, in the treated seeds compared to the controls. Along with the yield increase, there were notable elevations in primary metabolites such as total sugars, proteins, and amino acids. Furthermore, secondary metabolites, including essential oils (EOs), alkaloids, steroids, phenols, and vitamins (e.g., tocopherol and ascorbic acid), also showed significant increases. Notably, the EO constituents showed varying levels of enhancements, with the highest increases in β-pinene (186.2%) and carvacrol (49.2%). Moreover, the treated seeds exhibited improved biological activity, as evidenced by their anti-oxidant (anti-lipid peroxidation and DPPH assays) and anti-microbial properties compared to the controls. The study reported a positive biofortification effect of the Actinopolyspora sp. JTT-01 strain on enhancing caraway seed’s quality and yield. However, additional field trials are needed to evaluate the commercial biofertilization capacity of this strain for caraway and other plants.
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spelling doaj-art-e424212b9b004da4819a7096e367109c2025-08-20T02:20:01ZengBMCBMC Plant Biology1471-22292025-04-0125111510.1186/s12870-025-06137-1The potential biofortification role of Actinopolyspora sp. JTT-01 in enhancing the yield and tissue chemical composition of caraway plantsAhmed M. Mahmoud0Mohamed S. Sheteiwy1Ali El-Keblawy2Zaid Ulhassan3Maha H. Khalaf4Hussein S. Mohamed5Mohammad K. Okla6Amal Mohamed AlGarawi7Ahmed M. El-Sawah8Enas S. Ahmed9Ahmed M. Reyad10Department of Botany and Microbiology, Faculty of Science, Beni-Suef UniversityDepartment of Integrative Agriculture, College of Agriculture and Veterinary Medicine, United Arab Emirates UniversityDepartment of Applied Biology, College of Sciences, University of SharjahInstitute of Crop Science, Ministry of Agriculture and Rural Affairs, Key Laboratory of Spectroscopy Sensing, Zhejiang UniversityDepartment of Botany and Microbiology, Faculty of Science, Beni-Suef UniversityChemistry of Medicinal and Aromatic Plants Department, Research Institute of Medicinal and Aromatic Plants (RIMAP), Beni-Suef UniversityDepartment of Botany and Microbiology, College of Science, King Saud UniversityDepartment of Botany and Microbiology, College of Science, King Saud UniversityDepartment of Agricultural Microbiology, Faculty of Agriculture, Mansoura UniversityDepartment of Botany and Microbiology, Faculty of Science, Beni-Suef UniversityDepartment of Botany and Microbiology, Faculty of Science, Beni-Suef UniversityAbstract The need for improving plant production, nutritional value, and medicinal applications has become increasingly important due to the growing global population. The caraway (Carum carvi L) plant has been recognized for its broad range of nutritional and therapeutic uses. Consequently, this study aimed to increase caraway seeds’ nutritional and biological value. To achieve this, the Actinopolyspora sp. JTT-0 strain, isolated from the medicinal plant Tephrosia purpurea, was investigated for its potential biofortification role to enhance caraway yield and quality. Our results revealed significant improvements (p < 0.05) in various physical parameters, such as seed yield, pod length, and bulk density, in the treated seeds compared to the controls. Along with the yield increase, there were notable elevations in primary metabolites such as total sugars, proteins, and amino acids. Furthermore, secondary metabolites, including essential oils (EOs), alkaloids, steroids, phenols, and vitamins (e.g., tocopherol and ascorbic acid), also showed significant increases. Notably, the EO constituents showed varying levels of enhancements, with the highest increases in β-pinene (186.2%) and carvacrol (49.2%). Moreover, the treated seeds exhibited improved biological activity, as evidenced by their anti-oxidant (anti-lipid peroxidation and DPPH assays) and anti-microbial properties compared to the controls. The study reported a positive biofortification effect of the Actinopolyspora sp. JTT-01 strain on enhancing caraway seed’s quality and yield. However, additional field trials are needed to evaluate the commercial biofertilization capacity of this strain for caraway and other plants.https://doi.org/10.1186/s12870-025-06137-1ActinobacteriaCaraway seedsEndophytesEssential oils
spellingShingle Ahmed M. Mahmoud
Mohamed S. Sheteiwy
Ali El-Keblawy
Zaid Ulhassan
Maha H. Khalaf
Hussein S. Mohamed
Mohammad K. Okla
Amal Mohamed AlGarawi
Ahmed M. El-Sawah
Enas S. Ahmed
Ahmed M. Reyad
The potential biofortification role of Actinopolyspora sp. JTT-01 in enhancing the yield and tissue chemical composition of caraway plants
BMC Plant Biology
Actinobacteria
Caraway seeds
Endophytes
Essential oils
title The potential biofortification role of Actinopolyspora sp. JTT-01 in enhancing the yield and tissue chemical composition of caraway plants
title_full The potential biofortification role of Actinopolyspora sp. JTT-01 in enhancing the yield and tissue chemical composition of caraway plants
title_fullStr The potential biofortification role of Actinopolyspora sp. JTT-01 in enhancing the yield and tissue chemical composition of caraway plants
title_full_unstemmed The potential biofortification role of Actinopolyspora sp. JTT-01 in enhancing the yield and tissue chemical composition of caraway plants
title_short The potential biofortification role of Actinopolyspora sp. JTT-01 in enhancing the yield and tissue chemical composition of caraway plants
title_sort potential biofortification role of actinopolyspora sp jtt 01 in enhancing the yield and tissue chemical composition of caraway plants
topic Actinobacteria
Caraway seeds
Endophytes
Essential oils
url https://doi.org/10.1186/s12870-025-06137-1
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