Polysaccharides from Chinese Herbal Lycium barbarum Induced Systemic and Local Immune Responses in H22 Tumor-Bearing Mice

Lycium barbarum polysaccharide (LBP) is isolated from the fruit of Chinese herbal Lycium barbarum. Previous studies had demonstrated that LBP could inhibit tumor growth and enhance the immunity in mice. However, the effect of LBP on systemic and local immune responses in vivo, especially on phenotyp...

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Main Authors: Xiangliang Deng, Shuang Luo, Xia Luo, Minghua Hu, Fangli Ma, Yuanyuan Wang, Xiaoping Lai, Lian Zhou
Format: Article
Language:English
Published: Wiley 2018-01-01
Series:Journal of Immunology Research
Online Access:http://dx.doi.org/10.1155/2018/3431782
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author Xiangliang Deng
Shuang Luo
Xia Luo
Minghua Hu
Fangli Ma
Yuanyuan Wang
Xiaoping Lai
Lian Zhou
author_facet Xiangliang Deng
Shuang Luo
Xia Luo
Minghua Hu
Fangli Ma
Yuanyuan Wang
Xiaoping Lai
Lian Zhou
author_sort Xiangliang Deng
collection DOAJ
description Lycium barbarum polysaccharide (LBP) is isolated from the fruit of Chinese herbal Lycium barbarum. Previous studies had demonstrated that LBP could inhibit tumor growth and enhance the immunity in mice. However, the effect of LBP on systemic and local immune responses in vivo, especially on phenotypic and functional changes of T cells, is still largely unknown. In the present study, we investigated the effects of LBP on systemic and local T cell-dependent antitumor immune responses in H22 tumor-bearing mice. The results showed that LBP could inhibit the solid tumor growth in mice, but showed little effect on the body weight or spleen index. Furthermore, LBP could maintain high levels of T cells in peripheral blood (PB), tumor draining lymph node (TDLN), and tumor tissue, prevent the increase of Tregs while promote infiltration of CD8+ T cells in tumor tissue, inhibit the production of TGF-β1 and IL-10 in serum, decrease the exhaustion phenotype of T cells, and maintain cytotoxicity of lymphocytes. Taken together, our results demonstrated that LBP simultaneously induced systemic and local immune responses in H22 tumor-bearing mice by alleviating immunosuppression and maintaining antitumor immune responses in mice.
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issn 2314-8861
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language English
publishDate 2018-01-01
publisher Wiley
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series Journal of Immunology Research
spelling doaj-art-e421dcb778244f6b9fa99a8b53e348782025-08-20T02:21:34ZengWileyJournal of Immunology Research2314-88612314-71562018-01-01201810.1155/2018/34317823431782Polysaccharides from Chinese Herbal Lycium barbarum Induced Systemic and Local Immune Responses in H22 Tumor-Bearing MiceXiangliang Deng0Shuang Luo1Xia Luo2Minghua Hu3Fangli Ma4Yuanyuan Wang5Xiaoping Lai6Lian Zhou7School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaInfinitus Chinese Herbal Immunity Research Centre, Guangzhou 510600, ChinaInfinitus Chinese Herbal Immunity Research Centre, Guangzhou 510600, ChinaInfinitus Chinese Herbal Immunity Research Centre, Guangzhou 510600, ChinaDongguan Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Dongguan 523000, ChinaSchool of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaLycium barbarum polysaccharide (LBP) is isolated from the fruit of Chinese herbal Lycium barbarum. Previous studies had demonstrated that LBP could inhibit tumor growth and enhance the immunity in mice. However, the effect of LBP on systemic and local immune responses in vivo, especially on phenotypic and functional changes of T cells, is still largely unknown. In the present study, we investigated the effects of LBP on systemic and local T cell-dependent antitumor immune responses in H22 tumor-bearing mice. The results showed that LBP could inhibit the solid tumor growth in mice, but showed little effect on the body weight or spleen index. Furthermore, LBP could maintain high levels of T cells in peripheral blood (PB), tumor draining lymph node (TDLN), and tumor tissue, prevent the increase of Tregs while promote infiltration of CD8+ T cells in tumor tissue, inhibit the production of TGF-β1 and IL-10 in serum, decrease the exhaustion phenotype of T cells, and maintain cytotoxicity of lymphocytes. Taken together, our results demonstrated that LBP simultaneously induced systemic and local immune responses in H22 tumor-bearing mice by alleviating immunosuppression and maintaining antitumor immune responses in mice.http://dx.doi.org/10.1155/2018/3431782
spellingShingle Xiangliang Deng
Shuang Luo
Xia Luo
Minghua Hu
Fangli Ma
Yuanyuan Wang
Xiaoping Lai
Lian Zhou
Polysaccharides from Chinese Herbal Lycium barbarum Induced Systemic and Local Immune Responses in H22 Tumor-Bearing Mice
Journal of Immunology Research
title Polysaccharides from Chinese Herbal Lycium barbarum Induced Systemic and Local Immune Responses in H22 Tumor-Bearing Mice
title_full Polysaccharides from Chinese Herbal Lycium barbarum Induced Systemic and Local Immune Responses in H22 Tumor-Bearing Mice
title_fullStr Polysaccharides from Chinese Herbal Lycium barbarum Induced Systemic and Local Immune Responses in H22 Tumor-Bearing Mice
title_full_unstemmed Polysaccharides from Chinese Herbal Lycium barbarum Induced Systemic and Local Immune Responses in H22 Tumor-Bearing Mice
title_short Polysaccharides from Chinese Herbal Lycium barbarum Induced Systemic and Local Immune Responses in H22 Tumor-Bearing Mice
title_sort polysaccharides from chinese herbal lycium barbarum induced systemic and local immune responses in h22 tumor bearing mice
url http://dx.doi.org/10.1155/2018/3431782
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