Genomic Characterization of <i>Bacillus</i> sp. THPS1: A Hot Spring-Derived Species with Functional Features and Biotechnological Potential
<i>Bacillus</i> sp. THPS1 is a novel strain isolated from a high-temperature hot spring in Thailand, exhibiting distinctive genomic features that enable adaptation to an extreme environment. This study aimed to characterize the genomic and functional attributes of <i>Bacillus</i...
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2024-12-01
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| author | Samuel Mwakisha Mwamburi Sk Injamamul Islam Nguyen Dinh-Hung Orathai Dangsawat Rapeewan Sowanpreecha Luu Tang Phuc Khang Napatsorn Montha Phatthanaphong Therdtatha Sefti Heza Dwinanti Patima Permpoonpattana Nguyen Vu Linh |
| author_facet | Samuel Mwakisha Mwamburi Sk Injamamul Islam Nguyen Dinh-Hung Orathai Dangsawat Rapeewan Sowanpreecha Luu Tang Phuc Khang Napatsorn Montha Phatthanaphong Therdtatha Sefti Heza Dwinanti Patima Permpoonpattana Nguyen Vu Linh |
| author_sort | Samuel Mwakisha Mwamburi |
| collection | DOAJ |
| description | <i>Bacillus</i> sp. THPS1 is a novel strain isolated from a high-temperature hot spring in Thailand, exhibiting distinctive genomic features that enable adaptation to an extreme environment. This study aimed to characterize the genomic and functional attributes of <i>Bacillus</i> sp. THPS1 to understand its adaptation strategies and evaluate its potential for biotechnological applications. The draft genome is 5.38 Mbp with a GC content of 35.67%, encoding 5606 genes, including those linked to stress response and sporulation, which are essential for survival in high-temperature conditions. Phylogenetic analysis and average nucleotide identity (ANI) values confirmed its classification as a distinct species within the <i>Bacillus</i> genus. Pangenome analysis involving 19 others closely related thermophilic <i>Bacillus</i> species identified 1888 singleton genes associated with heat resistance, sporulation, and specialized metabolism, suggesting adaptation to nutrient-deficient, high-temperature environments. Genomic analysis revealed 12 biosynthetic gene clusters (BGCs), including those for polyketides and non-ribosomal peptides, highlighting its potential for synthesizing secondary metabolites that may facilitate its adaptation. Additionally, the presence of three <i>Siphoviridae</i> phage regions and 96 mobile genetic elements (MGEs) suggests significant genomic plasticity, whereas the existence of five CRISPR arrays implies an advanced defense mechanism against phage infections, contributing to genomic stability. The distinctive genomic features and functional capacities of <i>Bacillus</i> sp. THPS1 make it a promising candidate for biotechnological applications, particularly in the production of heat-stable enzymes and the development of resilient bioformulations. |
| format | Article |
| id | doaj-art-e41c45889fb64639bd091518142529f5 |
| institution | DOAJ |
| issn | 2076-2607 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-e41c45889fb64639bd091518142529f52025-08-20T02:57:16ZengMDPI AGMicroorganisms2076-26072024-12-011212247610.3390/microorganisms12122476Genomic Characterization of <i>Bacillus</i> sp. THPS1: A Hot Spring-Derived Species with Functional Features and Biotechnological PotentialSamuel Mwakisha Mwamburi0Sk Injamamul Islam1Nguyen Dinh-Hung2Orathai Dangsawat3Rapeewan Sowanpreecha4Luu Tang Phuc Khang5Napatsorn Montha6Phatthanaphong Therdtatha7Sefti Heza Dwinanti8Patima Permpoonpattana9Nguyen Vu Linh10Kenya Marine and Fisheries Research Institute, Mombasa 80100, KenyaDepartment of Fisheries and Marine Bioscience, Faculty of Biological Science and Technology, Jashore University of Science and Technology, Jashore 7408, BangladeshAquaculture Pathology Laboratory, School of Animal & Comparative Biomedical Sciences, The University of Arizona, Tucson, AZ 85721, USAScientific Laboratory and Equipment Center, Office of Surat Thani Campus, Prince of Songkla University, Surat Thani Campus, Surat Thani 84000, ThailandDepartment of Agricultural Science and Technology, Faculty of Innovative Agriculture, Fisheries and Food, Prince of Songkla University, Surat Thani Campus, Surat Thani 84000, ThailandDepartment of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, ThailandDivision of Biotechnology, School of Agro-Industry, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, ThailandDepartment of Aquaculture, Faculty of Agriculture, Sriwijaya University, Inderalaya 30662, IndonesiaDepartment of Agricultural Science and Technology, Faculty of Innovative Agriculture, Fisheries and Food, Prince of Songkla University, Surat Thani Campus, Surat Thani 84000, ThailandDepartment of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand<i>Bacillus</i> sp. THPS1 is a novel strain isolated from a high-temperature hot spring in Thailand, exhibiting distinctive genomic features that enable adaptation to an extreme environment. This study aimed to characterize the genomic and functional attributes of <i>Bacillus</i> sp. THPS1 to understand its adaptation strategies and evaluate its potential for biotechnological applications. The draft genome is 5.38 Mbp with a GC content of 35.67%, encoding 5606 genes, including those linked to stress response and sporulation, which are essential for survival in high-temperature conditions. Phylogenetic analysis and average nucleotide identity (ANI) values confirmed its classification as a distinct species within the <i>Bacillus</i> genus. Pangenome analysis involving 19 others closely related thermophilic <i>Bacillus</i> species identified 1888 singleton genes associated with heat resistance, sporulation, and specialized metabolism, suggesting adaptation to nutrient-deficient, high-temperature environments. Genomic analysis revealed 12 biosynthetic gene clusters (BGCs), including those for polyketides and non-ribosomal peptides, highlighting its potential for synthesizing secondary metabolites that may facilitate its adaptation. Additionally, the presence of three <i>Siphoviridae</i> phage regions and 96 mobile genetic elements (MGEs) suggests significant genomic plasticity, whereas the existence of five CRISPR arrays implies an advanced defense mechanism against phage infections, contributing to genomic stability. The distinctive genomic features and functional capacities of <i>Bacillus</i> sp. THPS1 make it a promising candidate for biotechnological applications, particularly in the production of heat-stable enzymes and the development of resilient bioformulations.https://www.mdpi.com/2076-2607/12/12/2476aquaculture<i>Bacillus</i> sp.genomic adaptationpangenome analysissporulation genes |
| spellingShingle | Samuel Mwakisha Mwamburi Sk Injamamul Islam Nguyen Dinh-Hung Orathai Dangsawat Rapeewan Sowanpreecha Luu Tang Phuc Khang Napatsorn Montha Phatthanaphong Therdtatha Sefti Heza Dwinanti Patima Permpoonpattana Nguyen Vu Linh Genomic Characterization of <i>Bacillus</i> sp. THPS1: A Hot Spring-Derived Species with Functional Features and Biotechnological Potential Microorganisms aquaculture <i>Bacillus</i> sp. genomic adaptation pangenome analysis sporulation genes |
| title | Genomic Characterization of <i>Bacillus</i> sp. THPS1: A Hot Spring-Derived Species with Functional Features and Biotechnological Potential |
| title_full | Genomic Characterization of <i>Bacillus</i> sp. THPS1: A Hot Spring-Derived Species with Functional Features and Biotechnological Potential |
| title_fullStr | Genomic Characterization of <i>Bacillus</i> sp. THPS1: A Hot Spring-Derived Species with Functional Features and Biotechnological Potential |
| title_full_unstemmed | Genomic Characterization of <i>Bacillus</i> sp. THPS1: A Hot Spring-Derived Species with Functional Features and Biotechnological Potential |
| title_short | Genomic Characterization of <i>Bacillus</i> sp. THPS1: A Hot Spring-Derived Species with Functional Features and Biotechnological Potential |
| title_sort | genomic characterization of i bacillus i sp thps1 a hot spring derived species with functional features and biotechnological potential |
| topic | aquaculture <i>Bacillus</i> sp. genomic adaptation pangenome analysis sporulation genes |
| url | https://www.mdpi.com/2076-2607/12/12/2476 |
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