Endophytic Bacterial Community Structure and Function Response of BLB Rice Leaves After Foliar Application of Cu-Ag Nanoparticles

Bacterial leaf blight (BLB) is a destructive disease caused by <i>Xanthomonas oryzae pv. oryzae</i> (<i>Xoo</i>). It has been proven that BLB adversely influences the growth and production of rice, resulting in substantial losses in yield. Nanoparticle–antimicrobial compounds...

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Main Authors: Weimin Ning, Mingxuan Li, Lei Jiang, Mei Yang, Maoyan Liu, Yong Liu
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Nanomaterials
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Online Access:https://www.mdpi.com/2079-4991/15/11/778
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author Weimin Ning
Mingxuan Li
Lei Jiang
Mei Yang
Maoyan Liu
Yong Liu
author_facet Weimin Ning
Mingxuan Li
Lei Jiang
Mei Yang
Maoyan Liu
Yong Liu
author_sort Weimin Ning
collection DOAJ
description Bacterial leaf blight (BLB) is a destructive disease caused by <i>Xanthomonas oryzae pv. oryzae</i> (<i>Xoo</i>). It has been proven that BLB adversely influences the growth and production of rice, resulting in substantial losses in yield. Nanoparticle–antimicrobial compounds possess excellent physicochemical properties, which have generated groundbreaking applications in protecting rice against BLB attacks. However, there is less research focused on the interaction between nanoparticles and the microbiome of BLB rice leaves, particularly the structure and function of endophytic bacteria, which are essential to plant health and pathogenesis. Therefore, the study explored how Cu-Ag nanoparticles influenced the endophytic bacteria’s composition and functions in healthy and BLB rice leaves. The data demonstrated that the relative abundance of beneficial bacteria, <i>Burkholderiales</i>, <i>Micrococcales</i>, and <i>Rhizobiales</i>, increased after the introduction of Cu-Ag nanoparticles on the leaves of BLB rice. The examination of PAL activity demonstrated that nanoparticles limited the spread of <i>Xoo</i> in rice leaves. Furthermore, endophytic community functional prediction demonstrated that nanoparticles may regulate the physiological process associated with potential stress resistance and growth-promoting function in the endophytic communities. This investigation may enhance the understanding of interactions between nanoparticles and the composition of rice endophytic microbiome, which can contribute to the exploration and application of nanomaterials in crop pathogen management.
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spelling doaj-art-e41aa52386c54f3bbc3d675dddc7a74e2025-08-20T02:32:56ZengMDPI AGNanomaterials2079-49912025-05-01151177810.3390/nano15110778Endophytic Bacterial Community Structure and Function Response of BLB Rice Leaves After Foliar Application of Cu-Ag NanoparticlesWeimin Ning0Mingxuan Li1Lei Jiang2Mei Yang3Maoyan Liu4Yong Liu5Agricultural Science College, Xichang University, Xichang 615000, ChinaAgricultural Science College, Xichang University, Xichang 615000, ChinaAgricultural Science College, Xichang University, Xichang 615000, ChinaAgricultural Science College, Xichang University, Xichang 615000, ChinaAgricultural Science College, Xichang University, Xichang 615000, ChinaLongping Branch, College of Biology, Hunan University, Changsha 410125, ChinaBacterial leaf blight (BLB) is a destructive disease caused by <i>Xanthomonas oryzae pv. oryzae</i> (<i>Xoo</i>). It has been proven that BLB adversely influences the growth and production of rice, resulting in substantial losses in yield. Nanoparticle–antimicrobial compounds possess excellent physicochemical properties, which have generated groundbreaking applications in protecting rice against BLB attacks. However, there is less research focused on the interaction between nanoparticles and the microbiome of BLB rice leaves, particularly the structure and function of endophytic bacteria, which are essential to plant health and pathogenesis. Therefore, the study explored how Cu-Ag nanoparticles influenced the endophytic bacteria’s composition and functions in healthy and BLB rice leaves. The data demonstrated that the relative abundance of beneficial bacteria, <i>Burkholderiales</i>, <i>Micrococcales</i>, and <i>Rhizobiales</i>, increased after the introduction of Cu-Ag nanoparticles on the leaves of BLB rice. The examination of PAL activity demonstrated that nanoparticles limited the spread of <i>Xoo</i> in rice leaves. Furthermore, endophytic community functional prediction demonstrated that nanoparticles may regulate the physiological process associated with potential stress resistance and growth-promoting function in the endophytic communities. This investigation may enhance the understanding of interactions between nanoparticles and the composition of rice endophytic microbiome, which can contribute to the exploration and application of nanomaterials in crop pathogen management.https://www.mdpi.com/2079-4991/15/11/778endophytic bacteriaCu-Ag nanoparticlesbacterial communityBLBrice
spellingShingle Weimin Ning
Mingxuan Li
Lei Jiang
Mei Yang
Maoyan Liu
Yong Liu
Endophytic Bacterial Community Structure and Function Response of BLB Rice Leaves After Foliar Application of Cu-Ag Nanoparticles
Nanomaterials
endophytic bacteria
Cu-Ag nanoparticles
bacterial community
BLB
rice
title Endophytic Bacterial Community Structure and Function Response of BLB Rice Leaves After Foliar Application of Cu-Ag Nanoparticles
title_full Endophytic Bacterial Community Structure and Function Response of BLB Rice Leaves After Foliar Application of Cu-Ag Nanoparticles
title_fullStr Endophytic Bacterial Community Structure and Function Response of BLB Rice Leaves After Foliar Application of Cu-Ag Nanoparticles
title_full_unstemmed Endophytic Bacterial Community Structure and Function Response of BLB Rice Leaves After Foliar Application of Cu-Ag Nanoparticles
title_short Endophytic Bacterial Community Structure and Function Response of BLB Rice Leaves After Foliar Application of Cu-Ag Nanoparticles
title_sort endophytic bacterial community structure and function response of blb rice leaves after foliar application of cu ag nanoparticles
topic endophytic bacteria
Cu-Ag nanoparticles
bacterial community
BLB
rice
url https://www.mdpi.com/2079-4991/15/11/778
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