Expression of Bax, Bcl-2 and Caspase-3 in spontaneous mammary gland tumors of rats
The investigation on the expression and interaction of Bax, Bcl-2 and Caspase-3 in the spontaneous mammary gland tumors of Sprague-Dawley (SD) rats was of great importance to provide a reference for the prevention and treatment of clinical mammary gland tumors.One hundred and thirty (half male and f...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2016-01-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2015.03.161 |
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| Summary: | The investigation on the expression and interaction of Bax, Bcl-2 and Caspase-3 in the spontaneous mammary gland tumors of Sprague-Dawley (SD) rats was of great importance to provide a reference for the prevention and treatment of clinical mammary gland tumors.One hundred and thirty (half male and female) SD rats were dissected after feeding 60 weeks, then the tumors were collected for pathological observation, and to measure the protein expression of Bax, Bcl-2 and Caspase-3 by immunohistochemistry (IHC), and the mRNA expression of the above factors were detected by real-time fluorescence quantitative PCR (RTFQ-PCR) method.Gross anatomy and pathology showed that 14 female rats had mammary gland tumors, the total incidence of tumors was 10.77% (14/130). However, in male rats, breast tumor had not been observed, meanwhile, the tumor occurrence rate in females was 21.54% (14/65), including 7 cases of breast fibroadenoma, 3 cases of mammary adenocarcinoma, 2 cases of mammary papillary carcinoma, and 2 cases of mammary invasive ductal carcinoma, respectively. IHC results showed that in the control group (n=5), the protein expression of Bax, Bcl-2 and Caspase-3 were 9.75±0.04, 8.13±0.35 and 6.60±0.04, respectively; in the benign group (n=7), the protein expression levels of these three factors were 8.16±0.16, 9.14±0.22 and 4.43±0.1, respectively, while in its malignant lesion group (n=7) were 6.49±0.32, 9.24±0.81 and 3.03±0.10, respectively. In addition, compared with the control group, the expression of Bax and Caspase-3 was significantly reduced (P<0.01) in the diseased groups, and the expression of Bcl-2 in lesion groups was significantly increased (P<0.05). Moreover, in the control group, the Bcl-2/Bax ratio was less than 1, which was more than 1 in benign and malignant groups. Compared with the IHC results, the RTFQ-PCR results showed that the mRNA expression of above factors were consistent with the protein expression, also showed highly significance (P<0.01).It is concluded that female rats have a high incidence of mammary tumors, which was not observed in males; Bcl-2, Bax and Caspase-3 are closely related to spontaneous mammary gland tumor, which may provide a new idea for diagnosis, treatment and prognosis of clinical breast tumor. |
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| ISSN: | 1008-9209 2097-5155 |