TaTCP6 is required for efficient and balanced utilization of nitrate and phosphorus in wheat
Abstract High crop yields require adequate nutrients, particularly nitrate (N) and phosphorus (P). Identifying regulators for efficient N-P utilization is critical in wheat. To explore N-P interactions, we analyze root transcriptomes under varying N-P supplies and identify TaTCP6 as a potential regu...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-02-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-57008-0 |
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| author | Bin Liu Weiya Xu Yanxiao Niu Qiuyuan Li Beilu Cao Jingyi Qi Yidi Zhao Yilan Zhou Long Song Dongkai Cui Zhenshan Liu Mingming Xin Yingyin Yao Mingshan You Zhongfu Ni Qixin Sun Jiewen Xing |
| author_facet | Bin Liu Weiya Xu Yanxiao Niu Qiuyuan Li Beilu Cao Jingyi Qi Yidi Zhao Yilan Zhou Long Song Dongkai Cui Zhenshan Liu Mingming Xin Yingyin Yao Mingshan You Zhongfu Ni Qixin Sun Jiewen Xing |
| author_sort | Bin Liu |
| collection | DOAJ |
| description | Abstract High crop yields require adequate nutrients, particularly nitrate (N) and phosphorus (P). Identifying regulators for efficient N-P utilization is critical in wheat. To explore N-P interactions, we analyze root transcriptomes under varying N-P supplies and identify TaTCP6 as a potential regulator. Nitrate-stimulated TaTCP6 directly triggers the expression of genes related to nitrogen utilization. TaTCP6 competes with TaSPX1/4 for the release of TaPHR2, and also interacts with TaPHR2 to enhance the transactivation capacity of downstream genes. Thus, through the dual roles of TaTCP6, the TCP6-SPX-PHR2 module activates the expression of phosphorus starvation response (PSR) genes. Inhibiting TaTCP6 reduces N and P absorption, negatively impacting yield, while overexpressing TaTCP6 increases grain yield. Notably, overexpression of TaSPX1 suppresses nitrogen utilization genes, especially under low phosphorus conditions. In conclusion, our findings highlight the role of TaTCP6 in coordinating N and P utilization and propose a strategy to reduce fertilizer inputs for sustainable agriculture. |
| format | Article |
| id | doaj-art-e4136cd47c8c4c2da4f21f3346af977e |
| institution | OA Journals |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-e4136cd47c8c4c2da4f21f3346af977e2025-08-20T02:12:59ZengNature PortfolioNature Communications2041-17232025-02-0116111910.1038/s41467-025-57008-0TaTCP6 is required for efficient and balanced utilization of nitrate and phosphorus in wheatBin Liu0Weiya Xu1Yanxiao Niu2Qiuyuan Li3Beilu Cao4Jingyi Qi5Yidi Zhao6Yilan Zhou7Long Song8Dongkai Cui9Zhenshan Liu10Mingming Xin11Yingyin Yao12Mingshan You13Zhongfu Ni14Qixin Sun15Jiewen Xing16Frontiers Science Center for Molecular Design Breeding (MOE)，Key Laboratory of Crop Heterosis and Utilization (MOE), China Agricultural UniversityFrontiers Science Center for Molecular Design Breeding (MOE)，Key Laboratory of Crop Heterosis and Utilization (MOE), China Agricultural UniversityFrontiers Science Center for Molecular Design Breeding (MOE)，Key Laboratory of Crop Heterosis and Utilization (MOE), China Agricultural UniversityFrontiers Science Center for Molecular Design Breeding (MOE)，Key Laboratory of Crop Heterosis and Utilization (MOE), China Agricultural UniversityFrontiers Science Center for Molecular Design Breeding (MOE)，Key Laboratory of Crop Heterosis and Utilization (MOE), China Agricultural UniversityFrontiers Science Center for Molecular Design Breeding (MOE)，Key Laboratory of Crop Heterosis and Utilization (MOE), China Agricultural UniversityFrontiers Science Center for Molecular Design Breeding (MOE)，Key Laboratory of Crop Heterosis and Utilization (MOE), China Agricultural UniversityFrontiers Science Center for Molecular Design Breeding (MOE)，Key Laboratory of Crop Heterosis and Utilization (MOE), China Agricultural UniversityFrontiers Science Center for Molecular Design Breeding (MOE)，Key Laboratory of Crop Heterosis and Utilization (MOE), China Agricultural UniversityState Key Laboratory of Crop Stress Biology for Arid Areas, Institute of Future Agriculture, Northwest A&F UniversityState Key Laboratory of Crop Stress Biology for Arid Areas, Institute of Future Agriculture, Northwest A&F UniversityFrontiers Science Center for Molecular Design Breeding (MOE)，Key Laboratory of Crop Heterosis and Utilization (MOE), China Agricultural UniversityFrontiers Science Center for Molecular Design Breeding (MOE)，Key Laboratory of Crop Heterosis and Utilization (MOE), China Agricultural UniversityFrontiers Science Center for Molecular Design Breeding (MOE)，Key Laboratory of Crop Heterosis and Utilization (MOE), China Agricultural UniversityFrontiers Science Center for Molecular Design Breeding (MOE)，Key Laboratory of Crop Heterosis and Utilization (MOE), China Agricultural UniversityFrontiers Science Center for Molecular Design Breeding (MOE)，Key Laboratory of Crop Heterosis and Utilization (MOE), China Agricultural UniversityFrontiers Science Center for Molecular Design Breeding (MOE)，Key Laboratory of Crop Heterosis and Utilization (MOE), China Agricultural UniversityAbstract High crop yields require adequate nutrients, particularly nitrate (N) and phosphorus (P). Identifying regulators for efficient N-P utilization is critical in wheat. To explore N-P interactions, we analyze root transcriptomes under varying N-P supplies and identify TaTCP6 as a potential regulator. Nitrate-stimulated TaTCP6 directly triggers the expression of genes related to nitrogen utilization. TaTCP6 competes with TaSPX1/4 for the release of TaPHR2, and also interacts with TaPHR2 to enhance the transactivation capacity of downstream genes. Thus, through the dual roles of TaTCP6, the TCP6-SPX-PHR2 module activates the expression of phosphorus starvation response (PSR) genes. Inhibiting TaTCP6 reduces N and P absorption, negatively impacting yield, while overexpressing TaTCP6 increases grain yield. Notably, overexpression of TaSPX1 suppresses nitrogen utilization genes, especially under low phosphorus conditions. In conclusion, our findings highlight the role of TaTCP6 in coordinating N and P utilization and propose a strategy to reduce fertilizer inputs for sustainable agriculture.https://doi.org/10.1038/s41467-025-57008-0 |
| spellingShingle | Bin Liu Weiya Xu Yanxiao Niu Qiuyuan Li Beilu Cao Jingyi Qi Yidi Zhao Yilan Zhou Long Song Dongkai Cui Zhenshan Liu Mingming Xin Yingyin Yao Mingshan You Zhongfu Ni Qixin Sun Jiewen Xing TaTCP6 is required for efficient and balanced utilization of nitrate and phosphorus in wheat Nature Communications |
| title | TaTCP6 is required for efficient and balanced utilization of nitrate and phosphorus in wheat |
| title_full | TaTCP6 is required for efficient and balanced utilization of nitrate and phosphorus in wheat |
| title_fullStr | TaTCP6 is required for efficient and balanced utilization of nitrate and phosphorus in wheat |
| title_full_unstemmed | TaTCP6 is required for efficient and balanced utilization of nitrate and phosphorus in wheat |
| title_short | TaTCP6 is required for efficient and balanced utilization of nitrate and phosphorus in wheat |
| title_sort | tatcp6 is required for efficient and balanced utilization of nitrate and phosphorus in wheat |
| url | https://doi.org/10.1038/s41467-025-57008-0 |
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