Catechin-Albumin Conjugates: Enhanced Antioxidant Capacity and Anticancer Effects
(+)-Catechin conjugated with human serum albumin (CT–HSA) was prepared and evaluated as a drug carrier bearing anticancer effects. It was found that 2.4 mol of CT was conjugate to 1 mol HSA. The CT–HSA has an antioxidant capacity of about 3.3 times the amount of CT in the conjugate. Intracellular in...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | International Journal of Food Science |
| Online Access: | http://dx.doi.org/10.1155/2022/1596687 |
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| author | Tooru Ooya Izumi Haraguchi |
| author_facet | Tooru Ooya Izumi Haraguchi |
| author_sort | Tooru Ooya |
| collection | DOAJ |
| description | (+)-Catechin conjugated with human serum albumin (CT–HSA) was prepared and evaluated as a drug carrier bearing anticancer effects. It was found that 2.4 mol of CT was conjugate to 1 mol HSA. The CT–HSA has an antioxidant capacity of about 3.3 times the amount of CT in the conjugate. Intracellular incorporation of the CT–HSA was analyzed by fluorescence-activated cell sorting (FACS) and confocal laser scanning microscopy (CLSM) measurements using fluorescein isothiocyanate (FITC)-labelled CT–HSA. The results indicated that the FITC-labelled CT–HSA was incorporated into HeLa cells in a concentration-dependent manner. The CT–HSA enhanced the binding of anticancer drugs (5-fluorouracil (5-Fu) and mitomycin C (MMC)) comparing with HSA, and the CT–HSA mixed with 5-Fu or MMC decreased significantly the HeLa cell viability as compared with the same concentration of each drug. In addition, intracellular reactive oxygen species (ROS) scavenging by the CT–HSA is likely to affect the anticancer effects. Thus, the CT–HSA enhanced anticancer drug efficacy in relation to controlling ROS-scavenging ability. |
| format | Article |
| id | doaj-art-e41090276666480cbf20ff54b9dc629c |
| institution | Kabale University |
| issn | 2314-5765 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Food Science |
| spelling | doaj-art-e41090276666480cbf20ff54b9dc629c2025-02-03T05:57:30ZengWileyInternational Journal of Food Science2314-57652022-01-01202210.1155/2022/1596687Catechin-Albumin Conjugates: Enhanced Antioxidant Capacity and Anticancer EffectsTooru Ooya0Izumi Haraguchi1Department of Chemical Science and EngineeringDepartment of Chemical Science and Engineering(+)-Catechin conjugated with human serum albumin (CT–HSA) was prepared and evaluated as a drug carrier bearing anticancer effects. It was found that 2.4 mol of CT was conjugate to 1 mol HSA. The CT–HSA has an antioxidant capacity of about 3.3 times the amount of CT in the conjugate. Intracellular incorporation of the CT–HSA was analyzed by fluorescence-activated cell sorting (FACS) and confocal laser scanning microscopy (CLSM) measurements using fluorescein isothiocyanate (FITC)-labelled CT–HSA. The results indicated that the FITC-labelled CT–HSA was incorporated into HeLa cells in a concentration-dependent manner. The CT–HSA enhanced the binding of anticancer drugs (5-fluorouracil (5-Fu) and mitomycin C (MMC)) comparing with HSA, and the CT–HSA mixed with 5-Fu or MMC decreased significantly the HeLa cell viability as compared with the same concentration of each drug. In addition, intracellular reactive oxygen species (ROS) scavenging by the CT–HSA is likely to affect the anticancer effects. Thus, the CT–HSA enhanced anticancer drug efficacy in relation to controlling ROS-scavenging ability.http://dx.doi.org/10.1155/2022/1596687 |
| spellingShingle | Tooru Ooya Izumi Haraguchi Catechin-Albumin Conjugates: Enhanced Antioxidant Capacity and Anticancer Effects International Journal of Food Science |
| title | Catechin-Albumin Conjugates: Enhanced Antioxidant Capacity and Anticancer Effects |
| title_full | Catechin-Albumin Conjugates: Enhanced Antioxidant Capacity and Anticancer Effects |
| title_fullStr | Catechin-Albumin Conjugates: Enhanced Antioxidant Capacity and Anticancer Effects |
| title_full_unstemmed | Catechin-Albumin Conjugates: Enhanced Antioxidant Capacity and Anticancer Effects |
| title_short | Catechin-Albumin Conjugates: Enhanced Antioxidant Capacity and Anticancer Effects |
| title_sort | catechin albumin conjugates enhanced antioxidant capacity and anticancer effects |
| url | http://dx.doi.org/10.1155/2022/1596687 |
| work_keys_str_mv | AT tooruooya catechinalbuminconjugatesenhancedantioxidantcapacityandanticancereffects AT izumiharaguchi catechinalbuminconjugatesenhancedantioxidantcapacityandanticancereffects |