Antimicrobial resistance levels amongst staphylococci isolated from clinical cases of bovine mastitis in Kosovo
Introduction: Mastitis is one of the most frequent and costly disease in cattle. We studied milk samples from cattle with mastitis from farms in Kosovo to identify mastitis-causing pathogens and possible effective antibiotics. Our ultimate goal is to help implement adequate antibiotic management and...
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The Journal of Infection in Developing Countries
2016-10-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/7912 |
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| author | Ibrahim Mehmeti Behlul Behluli Mergim Mestani Arsim Ademi Ingolf F Nes Dzung B Diep |
| author_facet | Ibrahim Mehmeti Behlul Behluli Mergim Mestani Arsim Ademi Ingolf F Nes Dzung B Diep |
| author_sort | Ibrahim Mehmeti |
| collection | DOAJ |
| description | Introduction: Mastitis is one of the most frequent and costly disease in cattle. We studied milk samples from cattle with mastitis from farms in Kosovo to identify mastitis-causing pathogens and possible effective antibiotics. Our ultimate goal is to help implement adequate antibiotic management and treatment practices in Kosovo
Methodology: A total of 152 milk samples were collected from cows with clinical mastitis from different farms in Kosovo. After identification of microorganisms, antibiotic susceptibility and the occurrence of enterotoxins was investigated.
Results: Staphylococci were found in 89 samples, of which 58 were coagulase negative and 31 coagulase positive. S. aureus was isolated from 27 samples, S. epidermidis from 25, and S. chromogenes from 15, while other species of staphylococci were isolated from the remaining 22 isolates. Interestingly, the bacterial diversity was different between cows in different periods of lactation and among different breeds. Most of the isolates (76/89) were resistant to two or more antibiotics. The highest resistance was to penicillin and ampicillin (> 65%), followed by tetracycline, oxacillin, streptomycin, chloramphenicol (> 23%), and less than 3% to erythromycin. Of the 89 isolates, 40 produced enterotoxins that were most frequently typed as A and C.
Conclusions: We detected human bacterial pathogens in the cultures of milk samples from cows with mastitis. The isolates demonstrated resistance to two or more antibiotics, some of which are frequently used to treat animal and human infections. We recommend increased control and more stringent use of antibiotics in veterinary as well as human medicine.
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| format | Article |
| id | doaj-art-e40c641e0617471eac766b3def0e653c |
| institution | DOAJ |
| issn | 1972-2680 |
| language | English |
| publishDate | 2016-10-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-e40c641e0617471eac766b3def0e653c2025-08-20T02:57:13ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802016-10-01101010.3855/jidc.7912Antimicrobial resistance levels amongst staphylococci isolated from clinical cases of bovine mastitis in KosovoIbrahim Mehmeti0Behlul Behluli1Mergim Mestani2Arsim Ademi3Ingolf F Nes4Dzung B Diep5Norwegian University of Life Sciences, Ås, NorwayFaculty of Agriculture and Veterinary, University of Prishtina Hasan Prishtina, Prishtina, KosovoFaculty of Agriculture and Veterinary, University of Prishtina Hasan Prishtina, Prishtina, KosovoNorwegian University of Life Sciences, Ås, NorwayNorwegian University of Life Sciences, Ås, NorwayNorwegian University of Life Sciences, Ås, NorwayIntroduction: Mastitis is one of the most frequent and costly disease in cattle. We studied milk samples from cattle with mastitis from farms in Kosovo to identify mastitis-causing pathogens and possible effective antibiotics. Our ultimate goal is to help implement adequate antibiotic management and treatment practices in Kosovo Methodology: A total of 152 milk samples were collected from cows with clinical mastitis from different farms in Kosovo. After identification of microorganisms, antibiotic susceptibility and the occurrence of enterotoxins was investigated. Results: Staphylococci were found in 89 samples, of which 58 were coagulase negative and 31 coagulase positive. S. aureus was isolated from 27 samples, S. epidermidis from 25, and S. chromogenes from 15, while other species of staphylococci were isolated from the remaining 22 isolates. Interestingly, the bacterial diversity was different between cows in different periods of lactation and among different breeds. Most of the isolates (76/89) were resistant to two or more antibiotics. The highest resistance was to penicillin and ampicillin (> 65%), followed by tetracycline, oxacillin, streptomycin, chloramphenicol (> 23%), and less than 3% to erythromycin. Of the 89 isolates, 40 produced enterotoxins that were most frequently typed as A and C. Conclusions: We detected human bacterial pathogens in the cultures of milk samples from cows with mastitis. The isolates demonstrated resistance to two or more antibiotics, some of which are frequently used to treat animal and human infections. We recommend increased control and more stringent use of antibiotics in veterinary as well as human medicine. https://jidc.org/index.php/journal/article/view/7912bovine mastitisstaphylococcimolecular identificationantibiotic resistanceenterotoxins |
| spellingShingle | Ibrahim Mehmeti Behlul Behluli Mergim Mestani Arsim Ademi Ingolf F Nes Dzung B Diep Antimicrobial resistance levels amongst staphylococci isolated from clinical cases of bovine mastitis in Kosovo Journal of Infection in Developing Countries bovine mastitis staphylococci molecular identification antibiotic resistance enterotoxins |
| title | Antimicrobial resistance levels amongst staphylococci isolated from clinical cases of bovine mastitis in Kosovo |
| title_full | Antimicrobial resistance levels amongst staphylococci isolated from clinical cases of bovine mastitis in Kosovo |
| title_fullStr | Antimicrobial resistance levels amongst staphylococci isolated from clinical cases of bovine mastitis in Kosovo |
| title_full_unstemmed | Antimicrobial resistance levels amongst staphylococci isolated from clinical cases of bovine mastitis in Kosovo |
| title_short | Antimicrobial resistance levels amongst staphylococci isolated from clinical cases of bovine mastitis in Kosovo |
| title_sort | antimicrobial resistance levels amongst staphylococci isolated from clinical cases of bovine mastitis in kosovo |
| topic | bovine mastitis staphylococci molecular identification antibiotic resistance enterotoxins |
| url | https://jidc.org/index.php/journal/article/view/7912 |
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