Comparison of the Antimicrobial Effect of Hydrogen Water and Chlorhexidine Mouth rinse in Toothbrush Disinfection Among Dental Students

Background: Toothbrushes are essential for eliminating dental biofilm and preventing caries and periodontal disease. Regular disinfecting is necessary to maintain a clean toothbrush. Chlorhexidine is the gold standard, but it may be resistant to periodontal pathogens. Hydrogen-rich water, due to its...

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Main Authors: C. Vaishnavi, Gayathri P. Elangovan, Mohan Thirumal, S. Vaishnavi Prashanth, D. Deepika, T.G. Pragathi
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2025-05-01
Series:Journal of Pharmacy and Bioallied Sciences
Subjects:
Online Access:https://journals.lww.com/10.4103/jpbs.jpbs_1565_24
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author C. Vaishnavi
Gayathri P. Elangovan
Mohan Thirumal
S. Vaishnavi Prashanth
D. Deepika
T.G. Pragathi
author_facet C. Vaishnavi
Gayathri P. Elangovan
Mohan Thirumal
S. Vaishnavi Prashanth
D. Deepika
T.G. Pragathi
author_sort C. Vaishnavi
collection DOAJ
description Background: Toothbrushes are essential for eliminating dental biofilm and preventing caries and periodontal disease. Regular disinfecting is necessary to maintain a clean toothbrush. Chlorhexidine is the gold standard, but it may be resistant to periodontal pathogens. Hydrogen-rich water, due to its antioxidant properties, may be beneficial against periodontal infections. Method: A study comparing hydrogen water and chlorhexidine as toothbrush disinfectants was conducted. Thirty dental students were divided into two groups: A and B. Group A students used hydrogen water and Group B used chlorhexidine mouth rinse as a disinfecting solution. They were instructed to brush twice a day for seven days and disinfect their toothbrushes with the disinfection solution for 10 minutes. Bristles from the collected toothbrush were sectioned and incubated for 24 hrs and colony-forming units (CFU) were counted. Result: The results showed that hydrogen water had significantly less colony-forming units in brushes disinfected with hydrogen water compared to chlorhexidine. Conclusion: Hydrogen water has better antimicrobial properties than chlorhexidine mouthwash. Therefore, hydrogen water is recommended as a regular disinfectant for toothbrushes.
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issn 0976-4879
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language English
publishDate 2025-05-01
publisher Wolters Kluwer Medknow Publications
record_format Article
series Journal of Pharmacy and Bioallied Sciences
spelling doaj-art-e4084b683448481b9bb758e1d5c5dc7a2025-08-20T03:24:40ZengWolters Kluwer Medknow PublicationsJournal of Pharmacy and Bioallied Sciences0976-48790975-74062025-05-0117Suppl 1S528S53010.4103/jpbs.jpbs_1565_24Comparison of the Antimicrobial Effect of Hydrogen Water and Chlorhexidine Mouth rinse in Toothbrush Disinfection Among Dental StudentsC. VaishnaviGayathri P. ElangovanMohan ThirumalS. Vaishnavi PrashanthD. DeepikaT.G. PragathiBackground: Toothbrushes are essential for eliminating dental biofilm and preventing caries and periodontal disease. Regular disinfecting is necessary to maintain a clean toothbrush. Chlorhexidine is the gold standard, but it may be resistant to periodontal pathogens. Hydrogen-rich water, due to its antioxidant properties, may be beneficial against periodontal infections. Method: A study comparing hydrogen water and chlorhexidine as toothbrush disinfectants was conducted. Thirty dental students were divided into two groups: A and B. Group A students used hydrogen water and Group B used chlorhexidine mouth rinse as a disinfecting solution. They were instructed to brush twice a day for seven days and disinfect their toothbrushes with the disinfection solution for 10 minutes. Bristles from the collected toothbrush were sectioned and incubated for 24 hrs and colony-forming units (CFU) were counted. Result: The results showed that hydrogen water had significantly less colony-forming units in brushes disinfected with hydrogen water compared to chlorhexidine. Conclusion: Hydrogen water has better antimicrobial properties than chlorhexidine mouthwash. Therefore, hydrogen water is recommended as a regular disinfectant for toothbrushes.https://journals.lww.com/10.4103/jpbs.jpbs_1565_24chlorhexidinedisinfectionhydrogen watertoothbrush
spellingShingle C. Vaishnavi
Gayathri P. Elangovan
Mohan Thirumal
S. Vaishnavi Prashanth
D. Deepika
T.G. Pragathi
Comparison of the Antimicrobial Effect of Hydrogen Water and Chlorhexidine Mouth rinse in Toothbrush Disinfection Among Dental Students
Journal of Pharmacy and Bioallied Sciences
chlorhexidine
disinfection
hydrogen water
toothbrush
title Comparison of the Antimicrobial Effect of Hydrogen Water and Chlorhexidine Mouth rinse in Toothbrush Disinfection Among Dental Students
title_full Comparison of the Antimicrobial Effect of Hydrogen Water and Chlorhexidine Mouth rinse in Toothbrush Disinfection Among Dental Students
title_fullStr Comparison of the Antimicrobial Effect of Hydrogen Water and Chlorhexidine Mouth rinse in Toothbrush Disinfection Among Dental Students
title_full_unstemmed Comparison of the Antimicrobial Effect of Hydrogen Water and Chlorhexidine Mouth rinse in Toothbrush Disinfection Among Dental Students
title_short Comparison of the Antimicrobial Effect of Hydrogen Water and Chlorhexidine Mouth rinse in Toothbrush Disinfection Among Dental Students
title_sort comparison of the antimicrobial effect of hydrogen water and chlorhexidine mouth rinse in toothbrush disinfection among dental students
topic chlorhexidine
disinfection
hydrogen water
toothbrush
url https://journals.lww.com/10.4103/jpbs.jpbs_1565_24
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AT mohanthirumal comparisonoftheantimicrobialeffectofhydrogenwaterandchlorhexidinemouthrinseintoothbrushdisinfectionamongdentalstudents
AT svaishnaviprashanth comparisonoftheantimicrobialeffectofhydrogenwaterandchlorhexidinemouthrinseintoothbrushdisinfectionamongdentalstudents
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