The Research Progress of Single-Molecule Sequencing and Its Significance in Nucleic Acid Metrology
Single-molecule sequencing technology, a novel method for gene sequencing, utilizes nano-sized materials to detect electrical and fluorescent signals. Compared to traditional Sanger sequencing and next-generation sequencing technologies, it offers significant advantages, including ultra-long read le...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-12-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/15/1/4 |
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| author | Yajun Wang Jingjing Liu Zhendong Wang Mei Zhang Yongzhuo Zhang |
| author_facet | Yajun Wang Jingjing Liu Zhendong Wang Mei Zhang Yongzhuo Zhang |
| author_sort | Yajun Wang |
| collection | DOAJ |
| description | Single-molecule sequencing technology, a novel method for gene sequencing, utilizes nano-sized materials to detect electrical and fluorescent signals. Compared to traditional Sanger sequencing and next-generation sequencing technologies, it offers significant advantages, including ultra-long read lengths, rapid sequencing, and the absence of amplification steps, making it widely applicable across various fields. By examining the development and components of single-molecule sequencing technology, it becomes clear that its unique characteristics provide new opportunities for advancing metrological traceability. Notably, its direct detection capabilities offer a novel approach to nucleic acid metrology. This paper provides a detailed overview of library construction, signal generation and detection, and data analysis methods in single-molecule sequencing and discusses its implications for nucleic acid metrology. |
| format | Article |
| id | doaj-art-e402c62d4a694ff495a2448d12b37d8c |
| institution | Kabale University |
| issn | 2079-6374 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj-art-e402c62d4a694ff495a2448d12b37d8c2025-01-24T13:25:24ZengMDPI AGBiosensors2079-63742024-12-01151410.3390/bios15010004The Research Progress of Single-Molecule Sequencing and Its Significance in Nucleic Acid MetrologyYajun Wang0Jingjing Liu1Zhendong Wang2Mei Zhang3Yongzhuo Zhang4College of Information and Control Engineering, Jilin Institute of Chemical Technology, Jilin 132022, ChinaCollege of Information and Control Engineering, China Northeast Electric Power University, Jilin 132011, ChinaCenter for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, ChinaCenter for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, ChinaCenter for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, ChinaSingle-molecule sequencing technology, a novel method for gene sequencing, utilizes nano-sized materials to detect electrical and fluorescent signals. Compared to traditional Sanger sequencing and next-generation sequencing technologies, it offers significant advantages, including ultra-long read lengths, rapid sequencing, and the absence of amplification steps, making it widely applicable across various fields. By examining the development and components of single-molecule sequencing technology, it becomes clear that its unique characteristics provide new opportunities for advancing metrological traceability. Notably, its direct detection capabilities offer a novel approach to nucleic acid metrology. This paper provides a detailed overview of library construction, signal generation and detection, and data analysis methods in single-molecule sequencing and discusses its implications for nucleic acid metrology.https://www.mdpi.com/2079-6374/15/1/4single-molecule sequencingnucleic acid metrological traceabilitynanopore |
| spellingShingle | Yajun Wang Jingjing Liu Zhendong Wang Mei Zhang Yongzhuo Zhang The Research Progress of Single-Molecule Sequencing and Its Significance in Nucleic Acid Metrology Biosensors single-molecule sequencing nucleic acid metrological traceability nanopore |
| title | The Research Progress of Single-Molecule Sequencing and Its Significance in Nucleic Acid Metrology |
| title_full | The Research Progress of Single-Molecule Sequencing and Its Significance in Nucleic Acid Metrology |
| title_fullStr | The Research Progress of Single-Molecule Sequencing and Its Significance in Nucleic Acid Metrology |
| title_full_unstemmed | The Research Progress of Single-Molecule Sequencing and Its Significance in Nucleic Acid Metrology |
| title_short | The Research Progress of Single-Molecule Sequencing and Its Significance in Nucleic Acid Metrology |
| title_sort | research progress of single molecule sequencing and its significance in nucleic acid metrology |
| topic | single-molecule sequencing nucleic acid metrological traceability nanopore |
| url | https://www.mdpi.com/2079-6374/15/1/4 |
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