Early-life gut microbiome associates with positive vaccine take and shedding in neonatal schedule of the human neonatal rotavirus vaccine RV3-BB
Abstract Rotavirus vaccines are less effective in high mortality regions. A rotavirus vaccine administered at birth may overcome challenges to vaccine uptake posed by a complex gut microbiome. We investigated the association between the microbiome and vaccine responses following RV3-BB vaccine (G3P[...
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Nature Portfolio
2025-04-01
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| Online Access: | https://doi.org/10.1038/s41467-025-58632-6 |
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| author | Josef Wagner Amanda Handley Celeste M. Donato Eleanor A. Lyons Daniel Pavlic Darren Suryawijaya Ong Rhian Bonnici Nada Bogdanovic-Sakran Edward P. K. Parker Christina Bronowski Jarir At Thobari Cahya Dewi Satria Hera Nirwati Desiree Witte Khuzwayo C. Jere Ashley Mpakiza Emma Watts Ann Turner Karen Boniface Jonathan Mandolo Frances Justice Naor Bar-Zeev Miren Iturriza-Gomara Jim P. Buttery Nigel A. Cunliffe Yati Soenarto Julie E. Bines |
| author_facet | Josef Wagner Amanda Handley Celeste M. Donato Eleanor A. Lyons Daniel Pavlic Darren Suryawijaya Ong Rhian Bonnici Nada Bogdanovic-Sakran Edward P. K. Parker Christina Bronowski Jarir At Thobari Cahya Dewi Satria Hera Nirwati Desiree Witte Khuzwayo C. Jere Ashley Mpakiza Emma Watts Ann Turner Karen Boniface Jonathan Mandolo Frances Justice Naor Bar-Zeev Miren Iturriza-Gomara Jim P. Buttery Nigel A. Cunliffe Yati Soenarto Julie E. Bines |
| author_sort | Josef Wagner |
| collection | DOAJ |
| description | Abstract Rotavirus vaccines are less effective in high mortality regions. A rotavirus vaccine administered at birth may overcome challenges to vaccine uptake posed by a complex gut microbiome. We investigated the association between the microbiome and vaccine responses following RV3-BB vaccine (G3P[6]) administered in a neonatal schedule (dose 1: 0-5 days), or infant schedule (dose 1: 6-8 weeks) in Indonesia (Phase 2b efficacy study) (n = 478 samples/193 infants) (ACTRN12612001282875) and in Malawi (Immunigenicity study) (n = 355 samples/186 infants) (NCT03483116). Vaccine responses assessed using anti-rotavirus IgA seroconversion (IgA), stool shedding of vaccine virus and vaccine take (IgA seroconversion and/or shedding). Here we report, high alpha diversity, beta diversity differences and high abundance of Bacteroides is associated with positive vaccine take and shedding following RV3-BB administered in the neonatal schedule, but not with IgA seroconversion, or in the infant schedule. Higher alpha diversity was associated with shedding after three doses of RV3-BB in the neonatal schedule compared to non-shedders, or the placebo group. High abundance of Streptococcus and Staphylococcus is associated with no shedding in the neonatal schedule group. RV3-BB vaccine administered in a neonatal schedule modulates the early microbiome environment and presents a window of opportunity to optimise protection from rotavirus disease. |
| format | Article |
| id | doaj-art-e3fed057b7c6494d9a8900fa472351e2 |
| institution | DOAJ |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Nature Portfolio |
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| series | Nature Communications |
| spelling | doaj-art-e3fed057b7c6494d9a8900fa472351e22025-08-20T03:06:52ZengNature PortfolioNature Communications2041-17232025-04-0116112110.1038/s41467-025-58632-6Early-life gut microbiome associates with positive vaccine take and shedding in neonatal schedule of the human neonatal rotavirus vaccine RV3-BBJosef Wagner0Amanda Handley1Celeste M. Donato2Eleanor A. Lyons3Daniel Pavlic4Darren Suryawijaya Ong5Rhian Bonnici6Nada Bogdanovic-Sakran7Edward P. K. Parker8Christina Bronowski9Jarir At Thobari10Cahya Dewi Satria11Hera Nirwati12Desiree Witte13Khuzwayo C. Jere14Ashley Mpakiza15Emma Watts16Ann Turner17Karen Boniface18Jonathan Mandolo19Frances Justice20Naor Bar-Zeev21Miren Iturriza-Gomara22Jim P. Buttery23Nigel A. Cunliffe24Yati Soenarto25Julie E. Bines26Enteric Diseases, Murdoch Children’s Research InstituteEnteric Diseases, Murdoch Children’s Research InstituteEnteric Diseases, Murdoch Children’s Research InstituteEnteric Diseases, Murdoch Children’s Research InstituteEnteric Diseases, Murdoch Children’s Research InstituteEnteric Diseases, Murdoch Children’s Research InstituteEnteric Diseases, Murdoch Children’s Research InstituteEnteric Diseases, Murdoch Children’s Research InstituteDepartment of Infectious Disease Epidemiology and International Health, London School of Hygiene and Tropical MedicineInstitute of Infection, Veterinary and Ecological Sciences, University of LiverpoolDepartment of Pharmacology and Therapy, Faculty of Medicine, Nursing and Universitas Gadjah MadaPediatric Research Office, Department of Pediatrics, Faculty of Medicine, Nursing and Universitas Gadjah MadaDepartment of Microbiology, Faculty of Medicine, Nursing and Universitas Gadjah Mada, Yogyakarta, Indonesia, Faculty of Medicine, Nursing and Universitas Gadjah MadaInstitute of Infection, Veterinary and Ecological Sciences, University of LiverpoolInstitute of Infection, Veterinary and Ecological Sciences, University of LiverpoolMalawi Liverpool Wellcome Programme, BlantyreEnteric Diseases, Murdoch Children’s Research InstituteMalawi Liverpool Wellcome Programme, BlantyreEnteric Diseases, Murdoch Children’s Research InstituteMalawi Liverpool Wellcome Programme, BlantyreEnteric Diseases, Murdoch Children’s Research InstituteInstitute of Infection, Veterinary and Ecological Sciences, University of LiverpoolInstitute of Infection, Veterinary and Ecological Sciences, University of LiverpoolEnteric Diseases, Murdoch Children’s Research InstituteInstitute of Infection, Veterinary and Ecological Sciences, University of LiverpoolPediatric Research Office, Department of Pediatrics, Faculty of Medicine, Nursing and Universitas Gadjah MadaEnteric Diseases, Murdoch Children’s Research InstituteAbstract Rotavirus vaccines are less effective in high mortality regions. A rotavirus vaccine administered at birth may overcome challenges to vaccine uptake posed by a complex gut microbiome. We investigated the association between the microbiome and vaccine responses following RV3-BB vaccine (G3P[6]) administered in a neonatal schedule (dose 1: 0-5 days), or infant schedule (dose 1: 6-8 weeks) in Indonesia (Phase 2b efficacy study) (n = 478 samples/193 infants) (ACTRN12612001282875) and in Malawi (Immunigenicity study) (n = 355 samples/186 infants) (NCT03483116). Vaccine responses assessed using anti-rotavirus IgA seroconversion (IgA), stool shedding of vaccine virus and vaccine take (IgA seroconversion and/or shedding). Here we report, high alpha diversity, beta diversity differences and high abundance of Bacteroides is associated with positive vaccine take and shedding following RV3-BB administered in the neonatal schedule, but not with IgA seroconversion, or in the infant schedule. Higher alpha diversity was associated with shedding after three doses of RV3-BB in the neonatal schedule compared to non-shedders, or the placebo group. High abundance of Streptococcus and Staphylococcus is associated with no shedding in the neonatal schedule group. RV3-BB vaccine administered in a neonatal schedule modulates the early microbiome environment and presents a window of opportunity to optimise protection from rotavirus disease.https://doi.org/10.1038/s41467-025-58632-6 |
| spellingShingle | Josef Wagner Amanda Handley Celeste M. Donato Eleanor A. Lyons Daniel Pavlic Darren Suryawijaya Ong Rhian Bonnici Nada Bogdanovic-Sakran Edward P. K. Parker Christina Bronowski Jarir At Thobari Cahya Dewi Satria Hera Nirwati Desiree Witte Khuzwayo C. Jere Ashley Mpakiza Emma Watts Ann Turner Karen Boniface Jonathan Mandolo Frances Justice Naor Bar-Zeev Miren Iturriza-Gomara Jim P. Buttery Nigel A. Cunliffe Yati Soenarto Julie E. Bines Early-life gut microbiome associates with positive vaccine take and shedding in neonatal schedule of the human neonatal rotavirus vaccine RV3-BB Nature Communications |
| title | Early-life gut microbiome associates with positive vaccine take and shedding in neonatal schedule of the human neonatal rotavirus vaccine RV3-BB |
| title_full | Early-life gut microbiome associates with positive vaccine take and shedding in neonatal schedule of the human neonatal rotavirus vaccine RV3-BB |
| title_fullStr | Early-life gut microbiome associates with positive vaccine take and shedding in neonatal schedule of the human neonatal rotavirus vaccine RV3-BB |
| title_full_unstemmed | Early-life gut microbiome associates with positive vaccine take and shedding in neonatal schedule of the human neonatal rotavirus vaccine RV3-BB |
| title_short | Early-life gut microbiome associates with positive vaccine take and shedding in neonatal schedule of the human neonatal rotavirus vaccine RV3-BB |
| title_sort | early life gut microbiome associates with positive vaccine take and shedding in neonatal schedule of the human neonatal rotavirus vaccine rv3 bb |
| url | https://doi.org/10.1038/s41467-025-58632-6 |
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