Retrospective screening of acute undifferentiated fever serum samples with universal flavivirus primers
Introduction: Fever is a common symptom of many tropical diseases and in many cases the etiologic agent remains unidentified as a consequence of either the etiologic agent not being part of routine diagnostic screening or as a consequence of false negatives on standard diagnostic tests. Methodology...
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| Format: | Article |
| Language: | English |
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The Journal of Infection in Developing Countries
2015-07-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/5866 |
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| author | Sarawut Khongwichit Sirikwan Libsittikul Sutee Yoksan Prasert Auewarakul Yupin Suputtamongkol Duncan R. Smith |
| author_facet | Sarawut Khongwichit Sirikwan Libsittikul Sutee Yoksan Prasert Auewarakul Yupin Suputtamongkol Duncan R. Smith |
| author_sort | Sarawut Khongwichit |
| collection | DOAJ |
| description | Introduction: Fever is a common symptom of many tropical diseases and in many cases the etiologic agent remains unidentified as a consequence of either the etiologic agent not being part of routine diagnostic screening or as a consequence of false negatives on standard diagnostic tests.
Methodology: This study screened a well characterized panel of 274 serum samples collected on day of admission from adult patients with acute undifferentiated fever admitted to a hospital in Nakhon Ratchasima, Thailand by RT-PCR using pan-flavivirus degenerate primers.
Results: Subsequent clinical diagnosis was achieved for 38 of the patients, and included 19 cases of dengue fever. RT-PCR screening identified seven positive samples (2.5%) which were revealed by sequence analysis to be dengue virus 1 (2 cases), dengue virus 2 (2 cases) and dengue virus 3 (3 cases). Only 5 out of 19 (26%) serum samples from patients subsequently diagnosed with dengue were positive, but 2 samples which clinically remained undiagnosed were shown to be positive for dengue virus. Sequence analysis suggested that the dengue virus 3 cases occurred as a result of importation of a strain of dengue from India or China. No other flaviviruses were identified.
Conclusions: No evidence was found of other flaviviruses besides dengue circulating in this population. Despite improved diagnostic tests, cases of dengue are still evading correct diagnosis.
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| format | Article |
| id | doaj-art-e3fa9407e7ec4363836dcb8bccdcc995 |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2015-07-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-e3fa9407e7ec4363836dcb8bccdcc9952025-08-20T03:52:42ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802015-07-0190710.3855/jidc.5866Retrospective screening of acute undifferentiated fever serum samples with universal flavivirus primersSarawut Khongwichit0Sirikwan Libsittikul1Sutee Yoksan2Prasert Auewarakul3Yupin Suputtamongkol4Duncan R. Smith5Siriraj Hospital, Mahidol University, Bangkok, ThailandSiriraj Hospital, Mahidol University, Bangkok, ThailandSiriraj Hospital, Mahidol University, Bangkok, ThailandCenter for Emerging and Neglected Infectious Diseases, Mahidol University, Bangkok, ThailandSiriraj Hospital, Mahidol University, Bangkok, ThailandSiriraj Hospital, Mahidol University, Bangkok, ThailandIntroduction: Fever is a common symptom of many tropical diseases and in many cases the etiologic agent remains unidentified as a consequence of either the etiologic agent not being part of routine diagnostic screening or as a consequence of false negatives on standard diagnostic tests. Methodology: This study screened a well characterized panel of 274 serum samples collected on day of admission from adult patients with acute undifferentiated fever admitted to a hospital in Nakhon Ratchasima, Thailand by RT-PCR using pan-flavivirus degenerate primers. Results: Subsequent clinical diagnosis was achieved for 38 of the patients, and included 19 cases of dengue fever. RT-PCR screening identified seven positive samples (2.5%) which were revealed by sequence analysis to be dengue virus 1 (2 cases), dengue virus 2 (2 cases) and dengue virus 3 (3 cases). Only 5 out of 19 (26%) serum samples from patients subsequently diagnosed with dengue were positive, but 2 samples which clinically remained undiagnosed were shown to be positive for dengue virus. Sequence analysis suggested that the dengue virus 3 cases occurred as a result of importation of a strain of dengue from India or China. No other flaviviruses were identified. Conclusions: No evidence was found of other flaviviruses besides dengue circulating in this population. Despite improved diagnostic tests, cases of dengue are still evading correct diagnosis. https://jidc.org/index.php/journal/article/view/5866feverdengueserumflavivirusphylogenyRT-PCR |
| spellingShingle | Sarawut Khongwichit Sirikwan Libsittikul Sutee Yoksan Prasert Auewarakul Yupin Suputtamongkol Duncan R. Smith Retrospective screening of acute undifferentiated fever serum samples with universal flavivirus primers Journal of Infection in Developing Countries fever dengue serum flavivirus phylogeny RT-PCR |
| title | Retrospective screening of acute undifferentiated fever serum samples with universal flavivirus primers |
| title_full | Retrospective screening of acute undifferentiated fever serum samples with universal flavivirus primers |
| title_fullStr | Retrospective screening of acute undifferentiated fever serum samples with universal flavivirus primers |
| title_full_unstemmed | Retrospective screening of acute undifferentiated fever serum samples with universal flavivirus primers |
| title_short | Retrospective screening of acute undifferentiated fever serum samples with universal flavivirus primers |
| title_sort | retrospective screening of acute undifferentiated fever serum samples with universal flavivirus primers |
| topic | fever dengue serum flavivirus phylogeny RT-PCR |
| url | https://jidc.org/index.php/journal/article/view/5866 |
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