Gankyrin promotes the proliferation of gastric cancer and is associated with chemosensitivity

Although gankyrin is involved in the tumorigenicity and metastasis of some malignancies, the role of gankyrin in gastric cancer is not clear. In this study, we evaluated the function and mechanism of gankyrin in gastric cancer. The effects of gankyrin on gastric cancer growth, proliferation, and che...

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Main Authors: Yue-Can Zeng, Dan Sun, Wen-Hui Li, Jing Zhao, Yan Xin
Format: Article
Language:English
Published: SAGE Publishing 2017-06-01
Series:Tumor Biology
Online Access:https://doi.org/10.1177/1010428317704820
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author Yue-Can Zeng
Dan Sun
Wen-Hui Li
Jing Zhao
Yan Xin
author_facet Yue-Can Zeng
Dan Sun
Wen-Hui Li
Jing Zhao
Yan Xin
author_sort Yue-Can Zeng
collection DOAJ
description Although gankyrin is involved in the tumorigenicity and metastasis of some malignancies, the role of gankyrin in gastric cancer is not clear. In this study, we evaluated the function and mechanism of gankyrin in gastric cancer. The effects of gankyrin on gastric cancer growth, proliferation, and chemosensitivity were determined. Gankyrin expression was significantly increased in gastric cancer compared to non-cancerous tissues. This expression significantly enhanced cancer cell proliferation and growth in vitro and in vivo. Suppression of gankyrin downregulated cyclin D1, cyclin E, proliferating cell nuclear antigen, phosphoinositide 3-kinase, AKT, p-PI3K, and p-AKT but upregulated Rb, p53, and p27. However, gankyrin overexpression led to opposite results. Downregulation of gankyrin expression enhanced chemosensitivity to 5-fluorouracil and cisplatin by inducing cell apoptosis. However, upregulation of gankyrin expression led to the opposite outcomes. Gankyrin enhanced gastric cancer cell proliferation by regulating cell cycle–related proteins and by activating PI3K/AKT signaling pathway. Gankyrin played an important role in gastric carcinogenesis and could be a potential effective therapeutic target for enhancing chemosensitivity to 5-fluorouracil and cisplatin.
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spelling doaj-art-e3f40f1d22a5472695f9e2448374f6462025-08-20T03:19:07ZengSAGE PublishingTumor Biology1423-03802017-06-013910.1177/1010428317704820Gankyrin promotes the proliferation of gastric cancer and is associated with chemosensitivityYue-Can ZengDan SunWen-Hui LiJing ZhaoYan XinAlthough gankyrin is involved in the tumorigenicity and metastasis of some malignancies, the role of gankyrin in gastric cancer is not clear. In this study, we evaluated the function and mechanism of gankyrin in gastric cancer. The effects of gankyrin on gastric cancer growth, proliferation, and chemosensitivity were determined. Gankyrin expression was significantly increased in gastric cancer compared to non-cancerous tissues. This expression significantly enhanced cancer cell proliferation and growth in vitro and in vivo. Suppression of gankyrin downregulated cyclin D1, cyclin E, proliferating cell nuclear antigen, phosphoinositide 3-kinase, AKT, p-PI3K, and p-AKT but upregulated Rb, p53, and p27. However, gankyrin overexpression led to opposite results. Downregulation of gankyrin expression enhanced chemosensitivity to 5-fluorouracil and cisplatin by inducing cell apoptosis. However, upregulation of gankyrin expression led to the opposite outcomes. Gankyrin enhanced gastric cancer cell proliferation by regulating cell cycle–related proteins and by activating PI3K/AKT signaling pathway. Gankyrin played an important role in gastric carcinogenesis and could be a potential effective therapeutic target for enhancing chemosensitivity to 5-fluorouracil and cisplatin.https://doi.org/10.1177/1010428317704820
spellingShingle Yue-Can Zeng
Dan Sun
Wen-Hui Li
Jing Zhao
Yan Xin
Gankyrin promotes the proliferation of gastric cancer and is associated with chemosensitivity
Tumor Biology
title Gankyrin promotes the proliferation of gastric cancer and is associated with chemosensitivity
title_full Gankyrin promotes the proliferation of gastric cancer and is associated with chemosensitivity
title_fullStr Gankyrin promotes the proliferation of gastric cancer and is associated with chemosensitivity
title_full_unstemmed Gankyrin promotes the proliferation of gastric cancer and is associated with chemosensitivity
title_short Gankyrin promotes the proliferation of gastric cancer and is associated with chemosensitivity
title_sort gankyrin promotes the proliferation of gastric cancer and is associated with chemosensitivity
url https://doi.org/10.1177/1010428317704820
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AT wenhuili gankyrinpromotestheproliferationofgastriccancerandisassociatedwithchemosensitivity
AT jingzhao gankyrinpromotestheproliferationofgastriccancerandisassociatedwithchemosensitivity
AT yanxin gankyrinpromotestheproliferationofgastriccancerandisassociatedwithchemosensitivity