An Assay System for Plate-based Detection of Endogenous Peptide:N-glycanase/NGLY1 Activity Using A Fluorescence-based Probe
Cytosolic peptide: N-glycanase (PNGase/NGLY1 in mammals), an amidase classified under EC:3.5.1.52, is a highly conserved enzyme across eukaryotes that catalyzes the removal of N-glycans from glycoproteins, converting N-glycosylated asparagine residues into aspartic acid. This enzyme also plays a rol...
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Main Authors: | Hiroto Hirayama, Tadashi Suzuki |
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Format: | Article |
Language: | English |
Published: |
Bio-protocol LLC
2025-01-01
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Series: | Bio-Protocol |
Online Access: | https://bio-protocol.org/en/bpdetail?id=5151&type=0 |
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