The Utilization of Formalin Fixed-Paraffin-Embedded Specimens in High Throughput Genomic Studies
High throughput genomic assays empower us to study the entire human genome in short time with reasonable cost. Formalin fixed-paraffin-embedded (FFPE) tissue processing remains the most economical approach for longitudinal tissue specimen storage. Therefore, the ability to apply high throughput geno...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2017/1926304 |
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| _version_ | 1849434635142955008 |
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| author | Pan Zhang Brian D. Lehmann Yu Shyr Yan Guo |
| author_facet | Pan Zhang Brian D. Lehmann Yu Shyr Yan Guo |
| author_sort | Pan Zhang |
| collection | DOAJ |
| description | High throughput genomic assays empower us to study the entire human genome in short time with reasonable cost. Formalin fixed-paraffin-embedded (FFPE) tissue processing remains the most economical approach for longitudinal tissue specimen storage. Therefore, the ability to apply high throughput genomic applications to FFPE specimens can expand clinical assays and discovery. Many studies have measured the accuracy and repeatability of data generated from FFPE specimens using high throughput genomic assays. Together, these studies demonstrate feasibility and provide crucial guidance for future studies using FFPE specimens. Here, we summarize the findings of these studies and discuss the limitations of high throughput data generated from FFPE specimens across several platforms that include microarray, high throughput sequencing, and NanoString. |
| format | Article |
| id | doaj-art-e3ee825fa4f74fb48be2e7b7c011f54f |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-e3ee825fa4f74fb48be2e7b7c011f54f2025-08-20T03:26:34ZengWileyInternational Journal of Genomics2314-436X2314-43782017-01-01201710.1155/2017/19263041926304The Utilization of Formalin Fixed-Paraffin-Embedded Specimens in High Throughput Genomic StudiesPan Zhang0Brian D. Lehmann1Yu Shyr2Yan Guo3Department of Cancer Biology, Vanderbilt University, Nashville, TN, USADepartment of Biochemistry, Vanderbilt University, Nashville, TN, USADepartment of Biostatistics, Vanderbilt University, Nashville, TN, USADepartment of Cancer Biology, Vanderbilt University, Nashville, TN, USAHigh throughput genomic assays empower us to study the entire human genome in short time with reasonable cost. Formalin fixed-paraffin-embedded (FFPE) tissue processing remains the most economical approach for longitudinal tissue specimen storage. Therefore, the ability to apply high throughput genomic applications to FFPE specimens can expand clinical assays and discovery. Many studies have measured the accuracy and repeatability of data generated from FFPE specimens using high throughput genomic assays. Together, these studies demonstrate feasibility and provide crucial guidance for future studies using FFPE specimens. Here, we summarize the findings of these studies and discuss the limitations of high throughput data generated from FFPE specimens across several platforms that include microarray, high throughput sequencing, and NanoString.http://dx.doi.org/10.1155/2017/1926304 |
| spellingShingle | Pan Zhang Brian D. Lehmann Yu Shyr Yan Guo The Utilization of Formalin Fixed-Paraffin-Embedded Specimens in High Throughput Genomic Studies International Journal of Genomics |
| title | The Utilization of Formalin Fixed-Paraffin-Embedded Specimens in High Throughput Genomic Studies |
| title_full | The Utilization of Formalin Fixed-Paraffin-Embedded Specimens in High Throughput Genomic Studies |
| title_fullStr | The Utilization of Formalin Fixed-Paraffin-Embedded Specimens in High Throughput Genomic Studies |
| title_full_unstemmed | The Utilization of Formalin Fixed-Paraffin-Embedded Specimens in High Throughput Genomic Studies |
| title_short | The Utilization of Formalin Fixed-Paraffin-Embedded Specimens in High Throughput Genomic Studies |
| title_sort | utilization of formalin fixed paraffin embedded specimens in high throughput genomic studies |
| url | http://dx.doi.org/10.1155/2017/1926304 |
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