Hook1 inhibits malignancy and epithelial–mesenchymal transition in hepatocellular carcinoma
Hook1 is a member of the hook family of coiled-coil proteins, which is recently found to be associated with malignant tumors. However, its biological function in hepatocellular carcinoma is yet unknown. Here, we evaluated the Hook1 levels in human hepatocellular carcinoma samples and matched peritum...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2017-07-01
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| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317711098 |
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| author | Xu Sun Qi Zhang Wei Chen Qida Hu Yu Lou Qi-Han Fu Jing-Ying Zhang Yi-Wen Chen Long-Yun Ye Yi Wang Shang-Zhi Xie Li-Qiang Hu Ting-Bo Liang Xue-Li Bai |
| author_facet | Xu Sun Qi Zhang Wei Chen Qida Hu Yu Lou Qi-Han Fu Jing-Ying Zhang Yi-Wen Chen Long-Yun Ye Yi Wang Shang-Zhi Xie Li-Qiang Hu Ting-Bo Liang Xue-Li Bai |
| author_sort | Xu Sun |
| collection | DOAJ |
| description | Hook1 is a member of the hook family of coiled-coil proteins, which is recently found to be associated with malignant tumors. However, its biological function in hepatocellular carcinoma is yet unknown. Here, we evaluated the Hook1 levels in human hepatocellular carcinoma samples and matched peritumoral tissues by real-time polymerase chain reaction. Small interfering RNA knockdown and a transforming growth factor-β-induced epithelial–mesenchymal transition model were employed to investigate the biological effects of Hook1 in hepatocellular carcinoma. Our results indicated that Hook1 levels were significantly lower in hepatocellular carcinoma tissues than in the peritumoral tissues. In addition, Hook1 expression was significantly associated with hepatocellular carcinoma malignancy. Hook1 was downregulated after transforming growth factor-β-induced epithelial–mesenchymal transition. Moreover, Hook1 knockdown promoted epithelial–mesenchymal transition and attenuated the sensitivity of hepatocellular carcinoma cells to doxorubicin. In summary, our results indicate that downregulation of Hook1 plays a pivotal role in hepatocellular carcinoma progression via epithelial–mesenchymal transition. Hook1 may be used as a novel marker and therapeutic molecular target in hepatocellular carcinoma. |
| format | Article |
| id | doaj-art-e3e84299efd6470fb9286fdcad433f67 |
| institution | DOAJ |
| issn | 1423-0380 |
| language | English |
| publishDate | 2017-07-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj-art-e3e84299efd6470fb9286fdcad433f672025-08-20T03:19:07ZengSAGE PublishingTumor Biology1423-03802017-07-013910.1177/1010428317711098Hook1 inhibits malignancy and epithelial–mesenchymal transition in hepatocellular carcinomaXu Sun0Qi Zhang1Wei Chen2Qida Hu3Yu Lou4Qi-Han Fu5Jing-Ying Zhang6Yi-Wen Chen7Long-Yun Ye8Yi Wang9Shang-Zhi Xie10Li-Qiang Hu11Ting-Bo Liang12Xue-Li Bai13Department of General Surgery, Huzhou Central Hospital, Zhejiang University Huzhou Hospital, Huzhou, ChinaDepartment of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaHook1 is a member of the hook family of coiled-coil proteins, which is recently found to be associated with malignant tumors. However, its biological function in hepatocellular carcinoma is yet unknown. Here, we evaluated the Hook1 levels in human hepatocellular carcinoma samples and matched peritumoral tissues by real-time polymerase chain reaction. Small interfering RNA knockdown and a transforming growth factor-β-induced epithelial–mesenchymal transition model were employed to investigate the biological effects of Hook1 in hepatocellular carcinoma. Our results indicated that Hook1 levels were significantly lower in hepatocellular carcinoma tissues than in the peritumoral tissues. In addition, Hook1 expression was significantly associated with hepatocellular carcinoma malignancy. Hook1 was downregulated after transforming growth factor-β-induced epithelial–mesenchymal transition. Moreover, Hook1 knockdown promoted epithelial–mesenchymal transition and attenuated the sensitivity of hepatocellular carcinoma cells to doxorubicin. In summary, our results indicate that downregulation of Hook1 plays a pivotal role in hepatocellular carcinoma progression via epithelial–mesenchymal transition. Hook1 may be used as a novel marker and therapeutic molecular target in hepatocellular carcinoma.https://doi.org/10.1177/1010428317711098 |
| spellingShingle | Xu Sun Qi Zhang Wei Chen Qida Hu Yu Lou Qi-Han Fu Jing-Ying Zhang Yi-Wen Chen Long-Yun Ye Yi Wang Shang-Zhi Xie Li-Qiang Hu Ting-Bo Liang Xue-Li Bai Hook1 inhibits malignancy and epithelial–mesenchymal transition in hepatocellular carcinoma Tumor Biology |
| title | Hook1 inhibits malignancy and epithelial–mesenchymal transition in hepatocellular carcinoma |
| title_full | Hook1 inhibits malignancy and epithelial–mesenchymal transition in hepatocellular carcinoma |
| title_fullStr | Hook1 inhibits malignancy and epithelial–mesenchymal transition in hepatocellular carcinoma |
| title_full_unstemmed | Hook1 inhibits malignancy and epithelial–mesenchymal transition in hepatocellular carcinoma |
| title_short | Hook1 inhibits malignancy and epithelial–mesenchymal transition in hepatocellular carcinoma |
| title_sort | hook1 inhibits malignancy and epithelial mesenchymal transition in hepatocellular carcinoma |
| url | https://doi.org/10.1177/1010428317711098 |
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