Differences of Clonogenic Mesenchymal Stem Cells on Immunomodulation of Lymphocyte Subsets
Mesenchymal stem cells (MSC) are a widely used population in cell therapy for their ability to differentiate into distinct tissues and more lately, for their immunomodulatory properties. However, the use of heterogeneous populations could be responsible for the nondesired outcomes reflected in the l...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2018/7232717 |
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| author | Pascual Martínez-Peinado Sandra Pascual-García Enrique Roche José Miguel Sempere-Ortells |
| author_facet | Pascual Martínez-Peinado Sandra Pascual-García Enrique Roche José Miguel Sempere-Ortells |
| author_sort | Pascual Martínez-Peinado |
| collection | DOAJ |
| description | Mesenchymal stem cells (MSC) are a widely used population in cell therapy for their ability to differentiate into distinct tissues and more lately, for their immunomodulatory properties. However, the use of heterogeneous populations could be responsible for the nondesired outcomes reflected in the literature. Here, we analyse the different capacities of five one-cell-derived MSC clones to exert their immunomodulation ex vivo. We assessed proliferation assays in cocultures of MSC clones and purified cluster of differentiation (CD)3+, CD4+, or CD8+ lymphocytes; analysed the regulatory T (Treg) cells fold change rate; determined the effects on viability of peripheral blood mononuclear cells (PBMC); and also measured the coculture cytokine profiles (Th1/Th2). Conditioned media (CM) of different clones were also used to perform both proliferation assays and to analyse Treg fold change. The five clones analysed in this work were able to generate heterogeneous environments. Different clones inhibited proliferation of CD3+ and CD4+ lymphocytes, with different intensities. Surprisingly, all clones promoted proliferation of CD8+ lymphocytes. Different MSC clones and their CM were able to increase the number of Treg with different intensities. Finally, different clones also promoted different effects on the viability of PBMC treated with ultraviolet light. Considering all these data together, it seems that different clones, even from the same donor, can promote a wide spectrum of responses from anti-inflammatory to proinflammatory character. This fact may be important to standardise the design of personalized cell therapy protocols, thus diminishing the aforementioned undesired outcomes existing nowadays in this type of therapies. |
| format | Article |
| id | doaj-art-e3e4e2ab778c4c158372c8333bab419f |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-e3e4e2ab778c4c158372c8333bab419f2025-08-20T03:26:33ZengWileyJournal of Immunology Research2314-88612314-71562018-01-01201810.1155/2018/72327177232717Differences of Clonogenic Mesenchymal Stem Cells on Immunomodulation of Lymphocyte SubsetsPascual Martínez-Peinado0Sandra Pascual-García1Enrique Roche2José Miguel Sempere-Ortells3Immunology Division, Biotechnology Department, University of Alicante, San Vicente del Raspeig, Alicante, SpainImmunology Division, Biotechnology Department, University of Alicante, San Vicente del Raspeig, Alicante, SpainBiochemistry and Cell Therapy Unit, Institute of Bioengineering, University Miguel Hernandez, Elche, Alicante, SpainImmunology Division, Biotechnology Department, University of Alicante, San Vicente del Raspeig, Alicante, SpainMesenchymal stem cells (MSC) are a widely used population in cell therapy for their ability to differentiate into distinct tissues and more lately, for their immunomodulatory properties. However, the use of heterogeneous populations could be responsible for the nondesired outcomes reflected in the literature. Here, we analyse the different capacities of five one-cell-derived MSC clones to exert their immunomodulation ex vivo. We assessed proliferation assays in cocultures of MSC clones and purified cluster of differentiation (CD)3+, CD4+, or CD8+ lymphocytes; analysed the regulatory T (Treg) cells fold change rate; determined the effects on viability of peripheral blood mononuclear cells (PBMC); and also measured the coculture cytokine profiles (Th1/Th2). Conditioned media (CM) of different clones were also used to perform both proliferation assays and to analyse Treg fold change. The five clones analysed in this work were able to generate heterogeneous environments. Different clones inhibited proliferation of CD3+ and CD4+ lymphocytes, with different intensities. Surprisingly, all clones promoted proliferation of CD8+ lymphocytes. Different MSC clones and their CM were able to increase the number of Treg with different intensities. Finally, different clones also promoted different effects on the viability of PBMC treated with ultraviolet light. Considering all these data together, it seems that different clones, even from the same donor, can promote a wide spectrum of responses from anti-inflammatory to proinflammatory character. This fact may be important to standardise the design of personalized cell therapy protocols, thus diminishing the aforementioned undesired outcomes existing nowadays in this type of therapies.http://dx.doi.org/10.1155/2018/7232717 |
| spellingShingle | Pascual Martínez-Peinado Sandra Pascual-García Enrique Roche José Miguel Sempere-Ortells Differences of Clonogenic Mesenchymal Stem Cells on Immunomodulation of Lymphocyte Subsets Journal of Immunology Research |
| title | Differences of Clonogenic Mesenchymal Stem Cells on Immunomodulation of Lymphocyte Subsets |
| title_full | Differences of Clonogenic Mesenchymal Stem Cells on Immunomodulation of Lymphocyte Subsets |
| title_fullStr | Differences of Clonogenic Mesenchymal Stem Cells on Immunomodulation of Lymphocyte Subsets |
| title_full_unstemmed | Differences of Clonogenic Mesenchymal Stem Cells on Immunomodulation of Lymphocyte Subsets |
| title_short | Differences of Clonogenic Mesenchymal Stem Cells on Immunomodulation of Lymphocyte Subsets |
| title_sort | differences of clonogenic mesenchymal stem cells on immunomodulation of lymphocyte subsets |
| url | http://dx.doi.org/10.1155/2018/7232717 |
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