Engineering high-frequency apomixis with normal seed production in hybrid rice
Summary: Clonal reproduction through seeds, also termed apomixis, has the potential to revolutionize agriculture by allowing hybrid crops to be clonally propagated. Although apomixis has been introduced into rice through de novo engineering in recent years, the poor fertility and low-frequency clona...
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| Language: | English |
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Elsevier
2024-12-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004224027068 |
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| author | Minglei Song Fei Li Zhen Chen Haonan Hou Yu Wang Huixia Liu Di Liu Junzhou Li Ting Peng Yafan Zhao Yanxiu Du Hongzheng Sun Changqing Du Jing Zhang Quanzhi Zhao Chunbo Miao |
| author_facet | Minglei Song Fei Li Zhen Chen Haonan Hou Yu Wang Huixia Liu Di Liu Junzhou Li Ting Peng Yafan Zhao Yanxiu Du Hongzheng Sun Changqing Du Jing Zhang Quanzhi Zhao Chunbo Miao |
| author_sort | Minglei Song |
| collection | DOAJ |
| description | Summary: Clonal reproduction through seeds, also termed apomixis, has the potential to revolutionize agriculture by allowing hybrid crops to be clonally propagated. Although apomixis has been introduced into rice through de novo engineering in recent years, the poor fertility and low-frequency clonal reproduction of synthetic apomicts hinder the application of apomixis in crop breeding. Here, in elite hybrid rice, we generated many apomicts, which produced clonal progeny with frequencies of > 95.0% and had high even normal fertility through combining enhanced rice BABYBOOM1 (BBM1)-induced parthenogenesis with the simultaneous inactivation of PAIR1, REC8, and OSD1. These synthetic apomicts maintained the agronomic traits of the elite hybrid rice. Our results indicate that fertility-normal hybrids with high penetrance of apomixis can be generated in rice, thereby laying an important foundation for the application of synthetic apomixis in crop breeding. |
| format | Article |
| id | doaj-art-e3d910d8747a4914a038fb5e202f0258 |
| institution | DOAJ |
| issn | 2589-0042 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-e3d910d8747a4914a038fb5e202f02582025-08-20T02:39:38ZengElsevieriScience2589-00422024-12-01271211147910.1016/j.isci.2024.111479Engineering high-frequency apomixis with normal seed production in hybrid riceMinglei Song0Fei Li1Zhen Chen2Haonan Hou3Yu Wang4Huixia Liu5Di Liu6Junzhou Li7Ting Peng8Yafan Zhao9Yanxiu Du10Hongzheng Sun11Changqing Du12Jing Zhang13Quanzhi Zhao14Chunbo Miao15Henan Key Laboratory of Rice Molecular Breeding and High Efficiency Production, College of Agronomy, Henan Agricultural University, Zhengzhou 450046, China; Institute of Advanced Biotechnology and School of Medicine, Southern University of Science and Technology, Shenzhen 518055, ChinaHenan Key Laboratory of Rice Molecular Breeding and High Efficiency Production, College of Agronomy, Henan Agricultural University, Zhengzhou 450046, ChinaHenan Key Laboratory of Rice Molecular Breeding and High Efficiency Production, College of Agronomy, Henan Agricultural University, Zhengzhou 450046, ChinaHenan Key Laboratory of Rice Molecular Breeding and High Efficiency Production, College of Agronomy, Henan Agricultural University, Zhengzhou 450046, ChinaHenan Key Laboratory of Rice Molecular Breeding and High Efficiency Production, College of Agronomy, Henan Agricultural University, Zhengzhou 450046, ChinaHenan Key Laboratory of Rice Molecular Breeding and High Efficiency Production, College of Agronomy, Henan Agricultural University, Zhengzhou 450046, ChinaHenan Key Laboratory of Rice Molecular Breeding and High Efficiency Production, College of Agronomy, Henan Agricultural University, Zhengzhou 450046, ChinaHenan Key Laboratory of Rice Molecular Breeding and High Efficiency Production, College of Agronomy, Henan Agricultural University, Zhengzhou 450046, ChinaHenan Key Laboratory of Rice Molecular Breeding and High Efficiency Production, College of Agronomy, Henan Agricultural University, Zhengzhou 450046, ChinaHenan Key Laboratory of Rice Molecular Breeding and High Efficiency Production, College of Agronomy, Henan Agricultural University, Zhengzhou 450046, ChinaHenan Key Laboratory of Rice Molecular Breeding and High Efficiency Production, College of Agronomy, Henan Agricultural University, Zhengzhou 450046, ChinaHenan Key Laboratory of Rice Molecular Breeding and High Efficiency Production, College of Agronomy, Henan Agricultural University, Zhengzhou 450046, ChinaHenan Key Laboratory of Rice Molecular Breeding and High Efficiency Production, College of Agronomy, Henan Agricultural University, Zhengzhou 450046, ChinaHenan Key Laboratory of Rice Molecular Breeding and High Efficiency Production, College of Agronomy, Henan Agricultural University, Zhengzhou 450046, ChinaHenan Key Laboratory of Rice Molecular Breeding and High Efficiency Production, College of Agronomy, Henan Agricultural University, Zhengzhou 450046, China; College of Agriculture, Guizhou University, Guiyang 550025, China; Corresponding authorHenan Key Laboratory of Rice Molecular Breeding and High Efficiency Production, College of Agronomy, Henan Agricultural University, Zhengzhou 450046, China; Corresponding authorSummary: Clonal reproduction through seeds, also termed apomixis, has the potential to revolutionize agriculture by allowing hybrid crops to be clonally propagated. Although apomixis has been introduced into rice through de novo engineering in recent years, the poor fertility and low-frequency clonal reproduction of synthetic apomicts hinder the application of apomixis in crop breeding. Here, in elite hybrid rice, we generated many apomicts, which produced clonal progeny with frequencies of > 95.0% and had high even normal fertility through combining enhanced rice BABYBOOM1 (BBM1)-induced parthenogenesis with the simultaneous inactivation of PAIR1, REC8, and OSD1. These synthetic apomicts maintained the agronomic traits of the elite hybrid rice. Our results indicate that fertility-normal hybrids with high penetrance of apomixis can be generated in rice, thereby laying an important foundation for the application of synthetic apomixis in crop breeding.http://www.sciencedirect.com/science/article/pii/S2589004224027068Biological sciencesMolecular biologyPlant biologyPlant genetics |
| spellingShingle | Minglei Song Fei Li Zhen Chen Haonan Hou Yu Wang Huixia Liu Di Liu Junzhou Li Ting Peng Yafan Zhao Yanxiu Du Hongzheng Sun Changqing Du Jing Zhang Quanzhi Zhao Chunbo Miao Engineering high-frequency apomixis with normal seed production in hybrid rice iScience Biological sciences Molecular biology Plant biology Plant genetics |
| title | Engineering high-frequency apomixis with normal seed production in hybrid rice |
| title_full | Engineering high-frequency apomixis with normal seed production in hybrid rice |
| title_fullStr | Engineering high-frequency apomixis with normal seed production in hybrid rice |
| title_full_unstemmed | Engineering high-frequency apomixis with normal seed production in hybrid rice |
| title_short | Engineering high-frequency apomixis with normal seed production in hybrid rice |
| title_sort | engineering high frequency apomixis with normal seed production in hybrid rice |
| topic | Biological sciences Molecular biology Plant biology Plant genetics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004224027068 |
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