In Situ Growth of The Interpenetrating Structure UiO‐66‐NH2/MWCNTs‐OH for The Detection of Phosphoproteins in Human Sweat

Abstract Phosphorylated proteins in human sweat are found to be closely associated with neurodegenerative diseases, including Alzheimer's disease. Real‐time, non‐invasive detection of changes in the types and levels of phosphorylated proteins in sweat is of significant clinical value for the pr...

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Main Authors: Zhiye Ying, Ce Bian, Lijuan Qiao, Bingxin Liu, Li Gao
Format: Article
Language:English
Published: Wiley-VCH 2025-04-01
Series:Advanced Sensor Research
Subjects:
Online Access:https://doi.org/10.1002/adsr.202400130
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author Zhiye Ying
Ce Bian
Lijuan Qiao
Bingxin Liu
Li Gao
author_facet Zhiye Ying
Ce Bian
Lijuan Qiao
Bingxin Liu
Li Gao
author_sort Zhiye Ying
collection DOAJ
description Abstract Phosphorylated proteins in human sweat are found to be closely associated with neurodegenerative diseases, including Alzheimer's disease. Real‐time, non‐invasive detection of changes in the types and levels of phosphorylated proteins in sweat is of significant clinical value for the prevention and diagnosis of these diseases. This research introduces an electrochemical sensor based on an in situ‐grown interpenetrating network structure of UiO‐66‐NH2/MWCNTs‐OH, specifically designed for detecting phosphorylated proteins in sweat. The sensor is tested with common phosphorylated and non‐phosphorylated proteins, demonstrating excellent selectivity, repeatability, reproducibility, and stability, with a limit of detection as low as 0.28 µmol L−1. Additionally, biotoxicity experiments conducted on SD rats and volunteers show that UiO‐66‐NH2/MWCNTs‐OH is biologically safe, making it suitable for use as a biosensor. Finally, the fabrication of the electrochemical sensing module provides a foundation for the development of subsequent wearable sensors.
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institution OA Journals
issn 2751-1219
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series Advanced Sensor Research
spelling doaj-art-e3d63667e6354415aa8b2f25326d4c592025-08-20T02:09:29ZengWiley-VCHAdvanced Sensor Research2751-12192025-04-0144n/an/a10.1002/adsr.202400130In Situ Growth of The Interpenetrating Structure UiO‐66‐NH2/MWCNTs‐OH for The Detection of Phosphoproteins in Human SweatZhiye Ying0Ce Bian1Lijuan Qiao2Bingxin Liu3Li Gao4School of Mechanical Engineering Qinghai University Xining 810016 P. R. ChinaSchool of Mechanical Engineering Qinghai University Xining 810016 P. R. ChinaResearch Center of Basic Medical Science Medical College Qinghai University Xining 810016 P. R. ChinaSchool of Mechanical Engineering Qinghai University Xining 810016 P. R. ChinaSchool of Mechanical Engineering Qinghai University Xining 810016 P. R. ChinaAbstract Phosphorylated proteins in human sweat are found to be closely associated with neurodegenerative diseases, including Alzheimer's disease. Real‐time, non‐invasive detection of changes in the types and levels of phosphorylated proteins in sweat is of significant clinical value for the prevention and diagnosis of these diseases. This research introduces an electrochemical sensor based on an in situ‐grown interpenetrating network structure of UiO‐66‐NH2/MWCNTs‐OH, specifically designed for detecting phosphorylated proteins in sweat. The sensor is tested with common phosphorylated and non‐phosphorylated proteins, demonstrating excellent selectivity, repeatability, reproducibility, and stability, with a limit of detection as low as 0.28 µmol L−1. Additionally, biotoxicity experiments conducted on SD rats and volunteers show that UiO‐66‐NH2/MWCNTs‐OH is biologically safe, making it suitable for use as a biosensor. Finally, the fabrication of the electrochemical sensing module provides a foundation for the development of subsequent wearable sensors.https://doi.org/10.1002/adsr.202400130MWCNTs‐OHphosphoproteinsweat sensorsUiO‐66‐NH2
spellingShingle Zhiye Ying
Ce Bian
Lijuan Qiao
Bingxin Liu
Li Gao
In Situ Growth of The Interpenetrating Structure UiO‐66‐NH2/MWCNTs‐OH for The Detection of Phosphoproteins in Human Sweat
Advanced Sensor Research
MWCNTs‐OH
phosphoprotein
sweat sensors
UiO‐66‐NH2
title In Situ Growth of The Interpenetrating Structure UiO‐66‐NH2/MWCNTs‐OH for The Detection of Phosphoproteins in Human Sweat
title_full In Situ Growth of The Interpenetrating Structure UiO‐66‐NH2/MWCNTs‐OH for The Detection of Phosphoproteins in Human Sweat
title_fullStr In Situ Growth of The Interpenetrating Structure UiO‐66‐NH2/MWCNTs‐OH for The Detection of Phosphoproteins in Human Sweat
title_full_unstemmed In Situ Growth of The Interpenetrating Structure UiO‐66‐NH2/MWCNTs‐OH for The Detection of Phosphoproteins in Human Sweat
title_short In Situ Growth of The Interpenetrating Structure UiO‐66‐NH2/MWCNTs‐OH for The Detection of Phosphoproteins in Human Sweat
title_sort in situ growth of the interpenetrating structure uio 66 nh2 mwcnts oh for the detection of phosphoproteins in human sweat
topic MWCNTs‐OH
phosphoprotein
sweat sensors
UiO‐66‐NH2
url https://doi.org/10.1002/adsr.202400130
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