Association of Vitamin E with Rapid Thawing on Goat Semen
The aim of this study was to evaluate the effects of vitamin E associated with rapid thawing on cryopreserved goat semen. Two bucks were used and eight ejaculates per animal were collected using artificial vagina. Semen was diluted with the following treatments: BIOXCELL (control), BIOXCELL + Equex...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/964172 |
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| author | Jurandy Mauro Penitente-Filho Fabrício Albani Oliveira Carolina Rodriguez Jimenez Erly Carrascal Júlio César Oliveira Dias Gisele Dias Oliveira Renata Gomes Silveira Camila Oliveira Silveira Ciro Alexandre Alves Torres |
| author_facet | Jurandy Mauro Penitente-Filho Fabrício Albani Oliveira Carolina Rodriguez Jimenez Erly Carrascal Júlio César Oliveira Dias Gisele Dias Oliveira Renata Gomes Silveira Camila Oliveira Silveira Ciro Alexandre Alves Torres |
| author_sort | Jurandy Mauro Penitente-Filho |
| collection | DOAJ |
| description | The aim of this study was to evaluate the effects of vitamin E associated with rapid thawing on cryopreserved goat semen. Two bucks were used and eight ejaculates per animal were collected using artificial vagina. Semen was diluted with the following treatments: BIOXCELL (control), BIOXCELL + Equex (sodium lauryl sulphate) and BIOXCELL + vitamin E 100 μM. Semen was packaged into 0.25 mL straws and cooled at 5°C for 1 hour. Freezing was performed in liquid nitrogen vapor (−155°C) during 15 minutes. Then, the straws were immersed in liquid nitrogen (−196°C). Straws were thawed at 38°C/60 seconds or at 60°C/7 seconds with immediate sperm analysis. Hypoosmotic swelling test was performed adding a 20 μL aliquot of thawed semen to 1 mL of hypoosmotic solution (100 mOsm·Kg−1) followed by incubation during 60 minutes in water bath (38°C). Vitamin E did not affect any studied parameters (P>0.05). Nevertheless, defrosting rate of 60°C/7 seconds improved sperm membrane functional integrity (P<0.05). Current knowledge about goat semen cryopreservation is not sufficient to ensure high post-thawing recovery rates; thus, this study brings important data about using antioxidants and different thawing rates on cryopreservation process. |
| format | Article |
| id | doaj-art-e3c8f5e14917428f8df593284d858a09 |
| institution | OA Journals |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-e3c8f5e14917428f8df593284d858a092025-08-20T02:21:24ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/964172964172Association of Vitamin E with Rapid Thawing on Goat SemenJurandy Mauro Penitente-Filho0Fabrício Albani Oliveira1Carolina Rodriguez Jimenez2Erly Carrascal3Júlio César Oliveira Dias4Gisele Dias Oliveira5Renata Gomes Silveira6Camila Oliveira Silveira7Ciro Alexandre Alves Torres8Department of Animal Science, Universidade Federal de Viçosa, 36570-900 Viçosa, MG, BrazilDepartment of Animal Science, Universidade Federal de Viçosa, 36570-900 Viçosa, MG, BrazilDepartment of Animal Science, Universidade Federal de Viçosa, 36570-900 Viçosa, MG, BrazilDepartment of Animal Science, Universidade Federal de Viçosa, 36570-900 Viçosa, MG, BrazilDepartment of Animal Science, Universidade Federal de Viçosa, 36570-900 Viçosa, MG, BrazilDepartment of Veterinary, Federal University of Viçosa, P. H. Rolfs Avenue, 36570-900 Viçosa, MG, BrazilFederal University of Espírito Santo, University Campus, 29500-000 Alegre, ES, BrazilDepartment of Veterinary, Federal University of Viçosa, P. H. Rolfs Avenue, 36570-900 Viçosa, MG, BrazilDepartment of Animal Science, Universidade Federal de Viçosa, 36570-900 Viçosa, MG, BrazilThe aim of this study was to evaluate the effects of vitamin E associated with rapid thawing on cryopreserved goat semen. Two bucks were used and eight ejaculates per animal were collected using artificial vagina. Semen was diluted with the following treatments: BIOXCELL (control), BIOXCELL + Equex (sodium lauryl sulphate) and BIOXCELL + vitamin E 100 μM. Semen was packaged into 0.25 mL straws and cooled at 5°C for 1 hour. Freezing was performed in liquid nitrogen vapor (−155°C) during 15 minutes. Then, the straws were immersed in liquid nitrogen (−196°C). Straws were thawed at 38°C/60 seconds or at 60°C/7 seconds with immediate sperm analysis. Hypoosmotic swelling test was performed adding a 20 μL aliquot of thawed semen to 1 mL of hypoosmotic solution (100 mOsm·Kg−1) followed by incubation during 60 minutes in water bath (38°C). Vitamin E did not affect any studied parameters (P>0.05). Nevertheless, defrosting rate of 60°C/7 seconds improved sperm membrane functional integrity (P<0.05). Current knowledge about goat semen cryopreservation is not sufficient to ensure high post-thawing recovery rates; thus, this study brings important data about using antioxidants and different thawing rates on cryopreservation process.http://dx.doi.org/10.1155/2014/964172 |
| spellingShingle | Jurandy Mauro Penitente-Filho Fabrício Albani Oliveira Carolina Rodriguez Jimenez Erly Carrascal Júlio César Oliveira Dias Gisele Dias Oliveira Renata Gomes Silveira Camila Oliveira Silveira Ciro Alexandre Alves Torres Association of Vitamin E with Rapid Thawing on Goat Semen The Scientific World Journal |
| title | Association of Vitamin E with Rapid Thawing on Goat Semen |
| title_full | Association of Vitamin E with Rapid Thawing on Goat Semen |
| title_fullStr | Association of Vitamin E with Rapid Thawing on Goat Semen |
| title_full_unstemmed | Association of Vitamin E with Rapid Thawing on Goat Semen |
| title_short | Association of Vitamin E with Rapid Thawing on Goat Semen |
| title_sort | association of vitamin e with rapid thawing on goat semen |
| url | http://dx.doi.org/10.1155/2014/964172 |
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