Molecular characterization of gliotoxin synthesis in a biofilm model of Aspergillus fumigatus
Mycelial growth as biofilm structures and the activation of secondary metabolism leading to the release of low-molecular-weight molecules (known as secondary metabolites), are among the previously described strategies used by the filamentous fungi Aspergillus fumigatus to adapt and survive. Our stud...
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| Format: | Article |
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Elsevier
2024-12-01
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| Series: | Biofilm |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590207524000637 |
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| author | Alicia Gomez-Lopez Candela Fernandez-Fernandez |
| author_facet | Alicia Gomez-Lopez Candela Fernandez-Fernandez |
| author_sort | Alicia Gomez-Lopez |
| collection | DOAJ |
| description | Mycelial growth as biofilm structures and the activation of secondary metabolism leading to the release of low-molecular-weight molecules (known as secondary metabolites), are among the previously described strategies used by the filamentous fungi Aspergillus fumigatus to adapt and survive. Our study unveils that A. fumigatus strains can activate mechanisms linked to the production of gliotoxin, a crucial metabolite for Aspergillus, in the established in vitro biofilm model. Gliotoxin production exhibits strain- and time-dependent patterns and is associated -in a coordinated manner-with the expression levels of several genes involved in its regulation and synthesis. The transcriptional study of some of these genes by qPCR shows temporal inter-strain differences, which correlate with those obtained when evaluating the amounts of metabolites produced. Given that A. fumigatus forms biofilm structures within the site of infection, understanding the regulation of gliotoxin biosynthesis may have a role in the evolution of Aspergillus infection and guide diagnostic and treatment strategies. |
| format | Article |
| id | doaj-art-e3b911fa075e46919de4b57fb34dc7d5 |
| institution | DOAJ |
| issn | 2590-2075 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biofilm |
| spelling | doaj-art-e3b911fa075e46919de4b57fb34dc7d52025-08-20T02:50:26ZengElsevierBiofilm2590-20752024-12-01810023810.1016/j.bioflm.2024.100238Molecular characterization of gliotoxin synthesis in a biofilm model of Aspergillus fumigatusAlicia Gomez-Lopez0Candela Fernandez-Fernandez1Mycology Reference and Research Laboratory, National Center for Microbiology (CNM-ISCIII), Madrid, Spain; Center for Biomedical Research in Network in Infectious Diseases (CIBERINFEC-CB21/13/00105), Carlos III Health Institute (ISCIII), Madrid, Spain; Corresponding author. Mycology Reference and Research Laboratory (National Center for Microbiology CNM-ISCIII, Carretera Majadahonda-Pozuelo Km 2, 28220, Majadahonda, Madrid, Spain.Mycology Reference and Research Laboratory, National Center for Microbiology (CNM-ISCIII), Madrid, SpainMycelial growth as biofilm structures and the activation of secondary metabolism leading to the release of low-molecular-weight molecules (known as secondary metabolites), are among the previously described strategies used by the filamentous fungi Aspergillus fumigatus to adapt and survive. Our study unveils that A. fumigatus strains can activate mechanisms linked to the production of gliotoxin, a crucial metabolite for Aspergillus, in the established in vitro biofilm model. Gliotoxin production exhibits strain- and time-dependent patterns and is associated -in a coordinated manner-with the expression levels of several genes involved in its regulation and synthesis. The transcriptional study of some of these genes by qPCR shows temporal inter-strain differences, which correlate with those obtained when evaluating the amounts of metabolites produced. Given that A. fumigatus forms biofilm structures within the site of infection, understanding the regulation of gliotoxin biosynthesis may have a role in the evolution of Aspergillus infection and guide diagnostic and treatment strategies.http://www.sciencedirect.com/science/article/pii/S2590207524000637Aspergillus fumigatusBiofilmSecondary metabolismGliotoxinLiquid chromatographyTranscriptional analysis |
| spellingShingle | Alicia Gomez-Lopez Candela Fernandez-Fernandez Molecular characterization of gliotoxin synthesis in a biofilm model of Aspergillus fumigatus Biofilm Aspergillus fumigatus Biofilm Secondary metabolism Gliotoxin Liquid chromatography Transcriptional analysis |
| title | Molecular characterization of gliotoxin synthesis in a biofilm model of Aspergillus fumigatus |
| title_full | Molecular characterization of gliotoxin synthesis in a biofilm model of Aspergillus fumigatus |
| title_fullStr | Molecular characterization of gliotoxin synthesis in a biofilm model of Aspergillus fumigatus |
| title_full_unstemmed | Molecular characterization of gliotoxin synthesis in a biofilm model of Aspergillus fumigatus |
| title_short | Molecular characterization of gliotoxin synthesis in a biofilm model of Aspergillus fumigatus |
| title_sort | molecular characterization of gliotoxin synthesis in a biofilm model of aspergillus fumigatus |
| topic | Aspergillus fumigatus Biofilm Secondary metabolism Gliotoxin Liquid chromatography Transcriptional analysis |
| url | http://www.sciencedirect.com/science/article/pii/S2590207524000637 |
| work_keys_str_mv | AT aliciagomezlopez molecularcharacterizationofgliotoxinsynthesisinabiofilmmodelofaspergillusfumigatus AT candelafernandezfernandez molecularcharacterizationofgliotoxinsynthesisinabiofilmmodelofaspergillusfumigatus |